Abstract: The present disclosure provides a method for evaluating prognosis in a patient with breast cancer, including the following steps: (a) detecting the expression level of pyruvate kinase in a sample from the patient; (b) detecting the expression level of O-linked ?-N-acetylglucosamine in the sample; and (c) evaluating a clinical treatment score post-5 years, wherein high expression levels of both the pyruvate kinase and the O-GlcNAc, and high score of the CTS5 indicate that the patient has a poor prognosis. The present disclosure also provides a kit for evaluating prognosis in a patient with breast cancer.