Abstract: A substrate cleaning apparatus includes a holder, a brush, a moving element, a driving element and a pressure sensor. The holder is used for disposing a substrate thereon. The moving element is connected to the brush. The driving element is connected to the moving element for proving a lateral pushing power to the moving element such that the moving element and the brush jointly move. The pressure sensor is disposed close to the moving element for sensing the lateral pushing power when the moving element moves to contact with the pressure sensor along a lateral direction, in which the brush moves to laterally push the substrate after the lateral pushing power is sensed to reach a first threshold value by the pressure sensor such that the brush is abutted on the substrate when the brush cleans the substrate.