Abstract: The present invention relates to DNA-barcoded recombinant nucleosomes and polynucleosomes that have been engineered for use as spike-in controls for the quantitative mapping of chromatin associated proteins using Chromatin ImmunoPrecipitation (ChIP) assays, tethered enzyme-based mapping assays, and other chromatin mapping assays. The invention further relates to methods of using the engineered DNA-barcoded recombinant nucleosomes in ChIP assays, tethered enzyme-based mapping assays, and other chromatin mapping assays.

Abstract: The present invention relates to the field of optical lenses, and provides a camera optical lens, including, from an object side to an image side, a first lens having a positive refractive power, a second lens a negative refractive power, a third lens having a positive refractive power, a fourth lens having a negative refractive power, and a fifth lens. At least one of the first lens to the fifth lens has a free-form surface, and the camera optical lens satisfies: 0?R7; and R10?0, where R7 denotes a central curvature radius of an object side surface of the fourth lens, and R10 denotes a central curvature radius of an image side surface of the fifth lens. The camera optical lens provided by the present invention satisfies design requirements of a long focal length and ultra-thinness while having excellent optical performance.

Abstract: A camera optical lens includes, from an object side to an image side, a first lens having a positive refractive power, a second lens having a negative refractive power, a third lens having a positive refractive power, a fourth lens having a negative refractive power, and a fifth lens, with at least one of the first to fifth lenses having a free-form surface, and satisfies: ?3.50?f4/f??0.35; ?8.00?R10/f??1.00; and 2.00?d8/d7?8.00, where f and f4 respectively denote focal lengths of the camera optical lens and the fourth lens; R10 denotes a central curvature radius of an image side surface of the fifth lens; d7 denotes an on-axis thickness of the fourth lens; and d8 denotes an on-axis distance from an image side surface of the fourth lens to an object side surface of the fifth lens, thereby having good optical performance while satisfying design requirements of a long focal length and ultra-thinness.

Abstract: This present invention relates to methods for carrying out chromatin mapping assays that use enzymes to incorporate barcoded DNA at targeted genomic regions followed by long-read sequencing (e.g., Third Generation Sequencing (TGS)). This approach enables the mapping of chromatin targets using TGS and can be used for a wide range of elements or features, including histone post-translational modifications, chromatin associated proteins, nucleosome positioning, and chromatin accessibility. The invention further relates to kits and reagents for carrying out the methods on chromatin samples that include one or more cells.

Abstract: The present invention provides a camera optical lens, including, from an object side to an image side, a first lens having a positive refractive power, a second lens a negative refractive power, a third lens having a positive refractive power, a fourth lens having a negative refractive power, and a fifth lens. At least one of the first lens to the fifth lens has a free-form surface, and the camera optical lens satisfies 0.50?f3/f?1.20; and 1.50?R7/R8, where f denotes a focal length of the camera optical lens, f3 denotes a focal length of the third lens, R7 is a central curvature radius of an object side surface of the fourth lens, and R8 is a central curvature radius of an image side surface of the fourth lens. The camera optical lens has excellent optical performance while meeting requirements of a long focal length and ultra-thinness.

Abstract: The present invention relates to DNA-barcoded recombinant nucleosomes and polynucleosomes that have been engineered for use as spike-in controls for chromatin accessibility assays, chromatin mapping assays, e.g., using tethered enzymes, as well as other chromatin assays. The invention further relates to methods of using the engineered DNA-barcoded recombinant nucleosomes in chromatin accessibility assays, chromatin mapping assays, as well as other chromatin assays.

Abstract: The present disclosure relates to the field of optical lenses and provides a camera optical lens. The camera optical lens includes, from an object side to an image side: an aperture; a first lens having a positive refractive power; a second lens having a negative refractive power; a third lens having a positive refractive power; a fourth lens having a positive refractive power; and a fifth lens having a negative refractive power. The camera optical lens satisfies following conditions: 0.80?f1/f?0.90; 70.00?f3/f?120.00; and ?450.00?(R5+R6)/(R5?R6)??430.00, where f denotes a focal length of the camera optical lens; f1 denotes a focal length of the first lens; f3 denotes a focal length of the third lens; R5 denotes a curvature radius of an object side surface of the third lens; and R6 denotes a curvature radius of an image side surface of the third lens.

Abstract: A method, device, and computer program storage product for generating a query to extract clinical features into a set of electronic medical record (EMR) tables based on clinical knowledge. A knowledge tree is constructed according to a set of clinical knowledge data. An EMR graph corresponding to a set of EMR tables is obtained. The EMR graph comprises at set of table nodes and a set of attribute nodes. The set of table nodes and the set of attribute nodes represent a structure of each EMR table in the set of EMR tables and a reference relationship among attributes of set of EMR tables. A plurality of sub-queries is generated based on the knowledge tree and the EMR graph. At least one query is generated by combining the plurality of sub-queries according to the knowledge tree.

Abstract: The present disclosure relates to the field of optical lenses, and discloses a camera optical lens. The camera optical lens includes eight lenses, and the eight lenses includes successively from an object side to an image side: a first lens having negative refractive power, a second lens having positive refractive power, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens, and an eighth lens. An object side surface of the eighth lens is a convex surface at a paraxial position, an image side surface thereof is a concave surface at a paraxial position, and at least one of the first lens to the eighth lens includes a free-form surface. The camera optical lens of the present disclosure has good optical performance while meeting design requirements of a large aperture, ultra-thinness, and a wide angle while.

Abstract: A camera optical lens includes, from an object side to an image side, a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens, and an eighth lens. At least one of the first lens to the eighth lens has a free-form surface, and the camera optical lens satisfies: ?3.50?f2/f1??1.50; and ?2.00?R9/R10??0.80, where f1 is a focal length of the first lens, f2 is a focal length of the second lens, R9 is a central curvature radius of an object side surface of the fifth lens, and R10 is a central curvature radius of an image side surface of the fifth lens. The camera optical lens has a large aperture, a wide angle, and ultra-thinness, as well as excellent optical performance.

Abstract: A camera optical lens includes, from an object side to an image side, a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens, and an eighth lens. At least one of the first lens to the eighth lens has a free-form surface, and the camera optical lens satisfies: ?7.50?f2/f6??1.50; and ?6.00?R1/R2??0.18, where f2 denotes a focal length of the second lens, f6 denotes a focal length of the sixth lens, R1 denotes a curvature radius of an object-side surface of the first lens, and R2 denotes a curvature radius of an image-side surface of the first lens. The camera optical lens has good optical performance, as well as a large aperture, ultra-thinness and a wide angle.

Abstract: A camera optical lens includes, from an object side to an image side, a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens, and an eighth lens. At least one of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens, or the eighth lens has a free-form surface. The first lens has a negative refractive power, and the third lens has a positive refractive power, an object-side surface of the second lens is convex at a paraxial position, and an image-side surface of the eighth lens is concave at the paraxial position. The camera optical lens has a wide angle and ultra-thinness, as well as excellent optical performance.

Abstract: A camera optical lens includes first to eighth lenses that are arranged sequentially from an object side to an image side. At least one of the first lens to the eighth lens has a free-form surface, and the camera optical lens satisfies: 1.30?f4/f?5.00; and 0?(R9+R10)/(R9?R10)?4.50, where f denotes a focal length of the camera optical lens, f4 denotes a focal length of the fourth lens, R9 denotes a curvature radius of an object-side surface of the fifth lens, and R10 denotes a curvature radius of an image-side surface of the fifth lens. The camera optical lens has a wide angle and is ultra-thin, as well as having excellent optical performance, and can effectively correct aberration and improve the performance of the optical system.

Abstract: A camera optical lens includes, from an object side to an image side, a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens, and an eighth lens. At least one of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens, or the eighth lens has a free-form surface, and the camera optical lens satisfies: 0.80?f6/f5?3.00; and ?6.00?R12/R11??2.50, where f5 denotes a focal length of the fifth lens, f6 denotes a focal length of the sixth lens, R11 denotes a central curvature radius of an object-side surface of the sixth lens, and R12 denotes a central curvature radius of an image-side surface of the sixth lens. The camera optical lens has a large aperture, a wide angle, and ultra-thinness, as well as excellent optical performance.

Abstract: A camera optical lens includes, from an object side to an image side: a first lens having a negative refractive power; a second lens having a positive refractive power; a third lens having a positive refractive power; a fourth lens having a positive refractive power; and a fifth lens having a negative refractive power. 2.50?f3/f?6.00, ?2.00?R4/R3??1.00, and 1.00?d1/d2?1.80. f denotes a focal length of the camera optical lens; f3 denotes a focal length of the third lens; R3 denotes a curvature radius of an object-side surface of the second lens; R4 denotes a curvature radius of an image-side surface of the second lens; d1 denotes an on-axis thickness of the first lens; d2 denotes an on-axis distance from an image-side surface of the first lens to an object-side surface of the second lens. The camera optical lens can achieve good optical performance while achieving ultra-thin, wide-angle lenses having large apertures.

Abstract: A camera optical lens is provided. The camera optical lens includes seven lenses, and the seven lenses are sequentially arranged from an object side to an image side, i.e., a first lens having a negative refractive power, a second lens having a positive refractive power, a third lens, a fourth lens having a negative refractive power, a fifth lens having a positive refractive power, a sixth lens having a positive refractive power, and a seventh lens having a negative refractive power. At least one of the first lens to the seventh lens includes a free-form surface. The camera optical lens according to the present disclosure can achieve good optical performance and meet the design requirements of being ultra-thin, and having a wide-angle and a large apertures.

Abstract: A camera optical lens is provided. The camera optical lens includes a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, and a seventh lens that are sequentially arranged from an object side to an image side. The second lens has a positive refractive power. At least one of the first lens to the seventh lens includes a free-form surface. The camera optical lens satisfies: 1.00?f2/f?4.00 and R1?0, where f denotes a focal length of the camera optical lens, f2 denotes a focal length of the second lens, and R1 denotes a central curvature radius of an object-side surface of the first lens. The camera optical lens can achieve good optical performance and meet the requirement of being ultra-thin and having a wide-angle and a large apertures.

Abstract: A method for testing cellular level water content and distribution in fruit and vegetable tissues based on Raman spectroscopy comprises preprocessing of samples, acquisition and preprocessing of imaging spectra, Gaussian peak-separation fitting of imaging spectra, pseudocolor imaging according to the fitting results, and visualization of distribution of water content and water bonding state at the cell level. The distribution of water content and water binding state is visualized at the cellular level in the fruit and vegetable tissues for the first time, and relatively reliable quantitative analysis results of the content of water with different bonding states according to the visualization imaging results is obtained. The new method for testing cellular level water content in fruit and vegetable tissues solves the current problem of not being able to detect cellular level water changes in fruit and vegetable processing, and has a good prospect for the research on fruit and vegetables processing.

Abstract: A camera optical lens includes, from an object side to an image side, a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, and a seventh lens. The camera optical lens satisfies: ?4.50?f4/f??2.00, and 0.65?d5/d6?19.50, where f denotes a focal length of the camera optical lens, f4 denotes a focal length of the fourth lens, d5 denotes an on-axis thickness of the third lens, and d6 denotes an on-axis distance from an image-side surface of the third lens to an object-side surface of the fourth lens. The camera optical lens has a good optical performance and meet the design requirements of being ultra-thin and having a wide angle and a large apertures.

Abstract: Provided is a camera optical lens including, sequentially from an object side to an image side: a first lens having a negative refractive power; a second lens having a refractive power; a third lens having a positive refractive power; a fourth lens having a negative refractive power; a fifth lens having a refractive power; a sixth lens having a positive refractive power; and a seventh lens having a negative refractive power. At least one of the first to seventh lenses comprises a free-form surface. The camera optical lens satisfies following conditions: ?3.00?f4/f3??1.00; 2.90?d9/d10?8.50; and 2.00?d11/d12?15.00. The camera optical lens can achieve high optical performance while satisfying design requirements for ultra-thin, wide-angle lenses.