Abstract: A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

Abstract: Provided are devices and methods for effecting processing of samples, including essentially isothermal amplification of nucleic acids, at multiple reaction locations in a single device. In some embodiments, the disclosed devices and methods provide for effecting parallel sample processing in several hundred locations on a single device.

Abstract: A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

Abstract: A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

Abstract: Provided herein are methods, compositions, and devices for the parallel handling of samples, such as cells or other biological samples. The methods, compositions, and devices are suited for multiple levels of analysis, including genetic and functional assays, of samples.

Abstract: Provided are devices comprising multivolume analysis regions, the devices being capable of supporting amplification, detection, and other processes. Also provided are related methods of detecting or estimating the presence nucleic acids, viral levels, and other biological markers of interest.

Abstract: Provided are devices comprising multivolume analysis regions, the devices being capable of supporting amplification, detection, and other processes. Also provided are related methods of detecting or estimating the presence nucleic acids, viral levels, and other biological markers of interest.

Abstract: A method for sampling and/or introducing a matter to an environment comprises introducing a first array of plugs through a first microchannel of a device into an exchange region of the device in which mass transport between the environment and the plug fluid of at least one plug in the first array of plugs occurs and a second array of plugs is formed. The exchange region is in fluid communication with the first microchannel. The method further comprises directing the second array of plugs into a second microchannel downstream of and in fluid communication with the exchange region.

Abstract: Provided are devices comprising multivolume analysis regions, the devices being capable of supporting amplification, detection, and other processes. Also provided are related methods of detecting or estimating the presence nucleic acids, viral levels, and other biological markers of interest.

Abstract: Provided are devices and methods for effecting processing of samples, including essentially isothermal amplification of nucleic acids, at multiple reaction locations in a single device. In some embodiments, the disclosed devices and methods provide for effecting parallel sample processing in several hundred locations on a single device.

Abstract: A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

Abstract: A method for sampling and/or introducing a matter to an environment comprises introducing a first array of plugs through a first microchannel of a device into an exchange region of the device in which mass transport between the environment and the plug fluid of at least one plug in the first array of plugs occurs and a second array of plugs is formed. The exchange region is in fluid communication with the first microchannel. The method further comprises directing the second array of plugs into a second microchannel downstream of and in fluid communication with the exchange region.

Abstract: A microfluidic system comprises a microchannel, a carrier fluid in the microchannel, and at least two plugs in the microchannel. Each plug comprises a plug fluid that is substantially immiscible with the carrier fluid. The microfluidic system further comprises at least one spacer in the microchannel between two plugs. Each spacer comprises a spacer fluid that is substantially immiscible with the carrier fluid and the plug fluid, and both of the following conditions are satisfied: (?c-r+?t-r>?c-t) and (?c-t+yt-r>yc-r), where ?c-r is the interfacial force between the carrier fluid and the plug fluid, ?t-r is the interfacial force between the spacer fluid and the plug fluid, and ?c-t is the interfacial force between the carrier fluid and the spacer fluid.