Abstract: A method for constructing a clinically-relevant large animal model of traumatic optic neuropathy, employing a goat with orbital anatomy and optic nerve structure similar to humans as a model animal, overing the defects existing in other animals as TON model animals. The target model goat is obtained by screening parameters, such as a maximum transverse diameter of a coronal plane of a body of sphenoid bone and a wall width of a sphenoid bone, thereby facilitating the exposure of an optic nerve in a optic-canal segment under a nasal endoscopic surgery and quantitative injury. With the optic nerve injury animal model, the clinical TON pathogenesis can be explored by studying the optic nerve at the injured optic canal. In the prepared TON animal model, the injured optic nerve is closer to the brain, which is beneficial to rebuilding the synaptic connection.

Abstract: A method for screening functional nucleic acid aptamers targeting GPCR proteins by combining cell sorting with functional sorting and an and application thereof, wherein the native conformation of target proteins is maintained through cell sorting for target proteins, and the obtained nucleic acid aptamers have better in vivo specificity and targeting property than the nucleic acid aptamers obtained by conventional protein SELEX to construct Cell-SELEX of model cells. Compared with the conventional Cell-SELEX technology with unclear targets, the present disclosure has a stronger pertinence. The present disclosure can directly obtain functional aptamers that can affect the signaling pathway of target proteins and the cell phenotypes only by performing one round of functional sorting in cells after screening out a series of target aptamers at one time. It has the advantages of strong pertinence, high affinity, high cost performance, low manpower cost and broad-spectrum universality, etc.

Abstract: The present application relates to the field of biotechnology, and in particular to a c-Met targeted aptamer drug conjugate. The drug conjugate c-Met-ApDC of the present application can achieve a cytotoxic IC50 of 100 nM in a cell with high expression of c-Met and a tumor model with high expression of c-Met, effectively inhibit a tumor in an animal model, achieve a tumor inhibition rate of 100% for a triple negative breast cancer PDX model, and gain complete regression of a transplanted tumor. The inhibition rate of the drug conjugate administrated subcutaneously to choroidal melanoma as a subcutaneous tumor by tail vein is up to 98.13%, and the inhibition rate of the drug conjugate injected intravitreally into intraocular choroidal melanoma in situ is 100%, which realizes complete regression of the transplanted tumor.

Abstract: A method for creating an animal model of traumatic optic nerve injury, including fully exposing an internal segment of an optic canal as well as adjacent anterior skull base, posterior ethmoid sinus and lateral sphenoid sinus walls through an ethmoid sinus-sphenoid sinus operation pathway under an endoscope, and impacting different sites of the internal segment of the optic canal with controllable impact force to cause optic nerve injury so as to prepare a controllable and quantifiable TONI bionic elastic injury animal model reflecting contusion to an internal segment of an optic canal in a human TONI clinical injury state. With less intracranial combined injury to the animal, the survival rate is high. Different sites of the optic canal are impacted with quantifiable elastic force for the quantitative and qualitative purposes with respect to the injured parts and the injury degree.

Abstract: A method for creating an animal model of indirect traumatic optic neuropathy, including fully exposing an internal segment of an optic canal as well as adjacent anterior skull base, posterior ethmoid sinus and lateral sphenoid sinus walls through an ethmoid sinus-sphenoid sinus operation pathway under an endoscope, and impacting different sites of the internal segment of the optic canal with controllable impact force to cause optic nerve injury so as to prepare a controllable and quantifiable ITON bionic elastic injury animal model reflecting contusion to an internal segment of an optic canal in a human ITON clinical injury state. With less intracranial combined injury to the animal, the survival rate is high. Different sites of the optic canal are impacted with quantifiable elastic force for the quantitative and qualitative purposes with respect to the injured parts and the injury degree.