Patents by Inventor Wenge Wang

Wenge Wang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200230256
    Abstract: An antibody production process in mammalian cells in which engineered unpaired cysteine residues are post-translationally modified and capped with particular chemical entities, which capped antibodies are well suited to further site-specific conjugation steps to form antibody-drug conjugates (ADCs) or protein drug conjugates; ADCs produced using these capped antibodies including in particular ADCs formed by the selective reduction of the capped antibodies' cysteine residues, and ADCs formed using chemical handles such as aldehyde/azide/alkyne biorthogonal groups, which permit additional drug conjugation chemistry; and uncapped antibodies produced by cells in low cysteine, cysteine and glutathione media, and ADCs produced via direct conjugation to these uncapped antibodies.
    Type: Application
    Filed: March 3, 2020
    Publication date: July 23, 2020
    Applicant: PFIZER INC.
    Inventors: Xiaotian Zhong, Amarnauth Shastrie Prashad, Ronald William Kriz, Tao He, Will Somers, Wenge Wang, Leo Joseph Letendre
  • Patent number: 10653794
    Abstract: An antibody production process in mammalian cells in which engineered unpaired cysteine residues are post-translationally modified and capped with particular chemical entities, which capped antibodies are well suited to further site-specific conjugation steps to form antibody-drug conjugates (ADCs) or protein drug conjugates; ADCs produced using these capped antibodies including in particular ADCs formed by the selective reduction of the capped antibodies' cysteine residues, and ADCs formed using chemical handles such as aldehyde/azide/alkyne biorthogonal groups, which permit additional drug conjugation chemistry; and uncapped antibodies produced by cells in low cysteine, cysteine and glutathione media, and ADCs produced via direct conjugation to these uncapped antibodies.
    Type: Grant
    Filed: August 9, 2016
    Date of Patent: May 19, 2020
    Assignee: Pfizer Inc.
    Inventors: Xiaotian Zhong, Amarnauth Shastrie Prashad, Ronald William Kriz, Tao He, Will Somers, Wenge Wang, Leo Joseph Letendre
  • Publication number: 20200107243
    Abstract: During disclosed communication techniques, an electronic device (such as an access point) may receive one or more packets from another electronic device, where the other electronic device has an established connection or association with the electronic device, and the one or more packets are communicated using a first band of frequencies. Then, the electronic device may determine to recommend a basic service set (BSS) transition from the first band of frequencies to at least a second band of frequencies. Moreover, the electronic device may provide a request packet to the other electronic device with a recommendation with one or more candidates for the BSS transition, such as a channel in the second band of frequencies. Next, the electronic device may receive a response packet from the other electronic device, where the response packet indicates a remedial action of the other electronic device in response to the request packet.
    Type: Application
    Filed: October 1, 2018
    Publication date: April 2, 2020
    Inventors: Wen Chen, Wenge Ren, Qingfeng Wang
  • Publication number: 20190112572
    Abstract: A cell culture medium comprising tyrosine at a concentration of at least 3 mM and polyvinylalcohol (PVA).
    Type: Application
    Filed: March 23, 2017
    Publication date: April 18, 2019
    Applicant: Pfizer Inc.
    Inventors: Bruno FIGUEROA, Yen-Tung Luan, Wenge Wang
  • Publication number: 20190030183
    Abstract: An antibody production process in mammalian cells in which engineered unpaired cysteine residues are post-translationally modified and capped with particular chemical entities, which capped antibodies are well suited to further site-specific conjugation steps to form antibody-drug conjugates (ADCs) or protein drug conjugates; ADCs produced using these capped antibodies including in particular ADCs formed by the selective reduction of the capped antibodies' cysteine residues, and ADCs formed using chemical handles such as aldehyde/azide/alkyne biorthogonal groups, which permit additional drug conjugation chemistry; and uncapped antibodies produced by cells in low cysteine, cysteine and glutathione media, and ADCs produced via direct conjugation to these uncapped antibodies.
    Type: Application
    Filed: August 9, 2016
    Publication date: January 31, 2019
    Applicant: PFIZER INC.
    Inventors: Xiaotian Zhong, Amarnauth Shastrie Prashad, Ronald William Kriz, Tao He, Will Somers, Wenge Wang, Leo Joseph Letendre
  • Publication number: 20150260087
    Abstract: The disclosure relates to a rotary piston, a piston engine comprising the rotary piston, and an engine device comprising the piston engine. The rotary piston comprises a piston body having a triangular vertical cross-section, mutually engaged large and small planetary gears fixed at the middle of the piston body, and a crankshaft running through the small planetary gear. The three angles of the triangular cross-section extend outwards to form protruded ends. The two sides of each protruded end form a compression groove and a combustion groove, respectively. The piston engine of the present disclosure comprises a shell, a crankshaft in the shell and two sets of rotary pistons, wherein the crankshaft has two ends and the two sets of rotary pistons are positioned symmetrically at the two ends of the crankshaft and separated by a holder positioned between the two sets of pistons.
    Type: Application
    Filed: August 20, 2014
    Publication date: September 17, 2015
    Inventor: Wenge WANG
  • Publication number: 20140322758
    Abstract: The present invention provides, among other things methods of increasing cell density, viability and/or titer in a cell culture including steps of adding a composition comprising iron to the cell culture.
    Type: Application
    Filed: October 9, 2012
    Publication date: October 30, 2014
    Inventors: Wenge Wang, Yen-Tung Luan, Denis Drapeau
  • Publication number: 20130084593
    Abstract: This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest.
    Type: Application
    Filed: July 30, 2012
    Publication date: April 4, 2013
    Applicant: Wyeth LLC
    Inventors: Yen-Tung Luan, Wenge Wang, Ryan Nolan, Denis Drapeau
  • Patent number: 8232075
    Abstract: This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest.
    Type: Grant
    Filed: November 8, 2007
    Date of Patent: July 31, 2012
    Assignee: Wyeth LLC
    Inventors: Yen-Tung Luan, Wenge Wang, Ryan Nolan, Denis Drapeau
  • Patent number: 8192951
    Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, cells expressing the protein or polypeptide of interest are grown in media that comprise a glycolysis-inhibiting substance. Additionally and/or alternatively, cells expressing the protein or polypeptide of interest are grown in media in which glutamine is limited. The use of such a system allows high levels of protein or polypeptide production and lessens accumulation of undesirable metabolic waste products such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, agricultural or other commercial compositions.
    Type: Grant
    Filed: November 2, 2007
    Date of Patent: June 5, 2012
    Assignee: Wyeth LLC
    Inventors: Wenge Wang, Yen Tung Luan
  • Patent number: 8039231
    Abstract: Methods and processes for improved recombinant protein production are provided. The methods are useful for production of growth factors, particularly those of the TGF-? superfamily, including bone morphogenetic proteins (BMPs), such as BMP-2. Suitable host cells are cultured in media where iron is present at a concentration of at least 2.25 ?M and if pyridoxal is present, it makes up less than 55% of the molar concentration of vitamin B6 in the media.
    Type: Grant
    Filed: April 16, 2009
    Date of Patent: October 18, 2011
    Assignee: Wyeth LLC
    Inventors: Yen-Tung Luan, Wenge Wang, Gregg Nyberg, Jose Manuel Gomes, Denis Drapeau, Terry Cardoza
  • Patent number: 7913483
    Abstract: On each draft element of a ring spinning frame, two pieces of short-staple roving from the roving bobbin enter into the draft mechanism to be drafted though a guide funnel in parallel, two pieces of filament are fed from the back of the front roller, and combine with two pieces of roving at front jaw respectively. The drafted two pieces of roving and filament are output from the front jaw and enter into the twisting triangle area to be twisted, and then are wound onto a yarn bobbin to produce yarn. Based on the relative position of the two pieces of roving and two pieces of filament, different yarns can be produced. Furthermore, multi-component yarn, such as core structure, wrapped structure, strand-like structure, can be produced on a ring spinning frame. The structure of yarn can be precisely determined. Special fiber yarn can be produced on traditional spinning frame.
    Type: Grant
    Filed: February 15, 2009
    Date of Patent: March 29, 2011
    Assignee: Shandong Ruyi Science & Technology Group
    Inventors: Yafu Qiu, Weilin Xu, Dong Qiu, Cailing Ding, Weigang Cui, Wenge Wang, Xiaolin Shen, Aiguo Yang, Baocang Li, Jianping Wang, Guang Qin
  • Publication number: 20100162677
    Abstract: On each draft element of a ring spinning frame, two pieces of short-staple roving from the roving bobbin enter into the draft mechanism to be drafted though a guide funnel in parallel, two pieces of filament are fed from the back of the front roller, and combine with two pieces of roving at front jaw respectively. The drafted two pieces of roving and filament are output from the front jaw and enter into the twisting triangle area to be twisted, and then are wound onto a yarn bobbin to produce yarn. Based on the relative position of the two pieces of roving and two pieces of filament, different yarns can be produced. Furthermore, multi-component yarn, such as core structure, wrapped structure, strand-like structure, can be produced on a ring spinning frame. The structure of yarn can be precisely determined. Special fiber yarn can be produced on traditional spinning frame.
    Type: Application
    Filed: February 15, 2009
    Publication date: July 1, 2010
    Inventors: Yafu Qiu, Weilin Xu, Dong Qiu, Cailing Ding, Weigang Cui, Wenge Wang, Xiaolin Shen, Aiguo Yang, Baocang Li, Jianping Wang, Guang Qin
  • Publication number: 20090317867
    Abstract: Methods and processes for improved recombinant protein production are provided. The methods are useful for production of growth factors, particularly those of the TGF-? superfamily, including bone morphogenetic proteins (BMPs), such as BMP-2. Suitable host cells are cultured in media where iron is present at a concentration of at least 2.25 ?M and if pyridoxal is present, it makes up less than 55% of the molar concentration of vitamin B6 in the media.
    Type: Application
    Filed: April 16, 2009
    Publication date: December 24, 2009
    Applicant: Wyeth, Madison, New Jersey
    Inventors: Yen-Tung Luan, Wenge Wang, Gregg Nyberg, Jose Manuel Gomes, Denis Drapeau, Terry Cardoza
  • Publication number: 20090280029
    Abstract: The present invention discloses a materials-processing system, which comprises an inputting subsystem, a processing apparatus coupled to the inputting subsystem and a collecting subsystem coupled to the processing apparatus. The inputting subsystem comprises three or more sample vessels, which can be connected to the processing apparatus. Since the processing system includes multiple sample vessels, which can be grouped into different groups so that each group contains two or more of the multiple sample vessels. High throughput materials transport can be realized by sequentially connecting different groups of sample vessels to the processing apparatus, thereby overcoming a limitation of the prior art that cannot continuously perform multiple batches of materials processing and improving material processing efficiency.
    Type: Application
    Filed: May 10, 2006
    Publication date: November 12, 2009
    Inventors: Youshu Kang, Guilin Wang, Wenge Wang, Wenhui Wang, Youqi Wang, Guangping Xie, Sibiao Xu, Xiaowen Zhu, Xianzhong Zhao
  • Publication number: 20080274507
    Abstract: Methods of producing a protein in cell culture comprising an anti-senescence compound, such as the antioxidant carnosine, are provided. According to teachings of the present invention, cells grown in a cell culture medium comprising an anti-senescence compound exhibit increased viability and productivity. Furthermore, cell cultures grown in the presence of an anti-senescence compound exhibit decreased levels of high molecular weight aggregates in the cell culture medium.
    Type: Application
    Filed: April 22, 2008
    Publication date: November 6, 2008
    Applicant: WYETH
    Inventors: Jose Manuel Gomes, Yen-Tung Luan, Gregory Walter Hiller, Wenge Wang
  • Publication number: 20080108553
    Abstract: This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest.
    Type: Application
    Filed: November 8, 2007
    Publication date: May 8, 2008
    Inventors: Yen-Tung Luan, Wenge Wang, Ryan Nolan, Denis Drapeau
  • Publication number: 20080108106
    Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, cells expressing the protein or polypeptide of interest are grown in media that comprise a glycolysis-inhibiting substance. Additionally and/or alternatively, cells expressing the protein or polypeptide of interest are grown in media in which glutamine is limited. The use of such a system allows high levels of protein or polypeptide production and lessens accumulation of undesirable metabolic waste products such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, agricultural or other commercial compositions.
    Type: Application
    Filed: November 2, 2007
    Publication date: May 8, 2008
    Applicant: Wyeth
    Inventors: Wenge Wang, Yen Luan
  • Patent number: 7335491
    Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.
    Type: Grant
    Filed: August 25, 2005
    Date of Patent: February 26, 2008
    Assignee: Wyeth Research Ireland Limited
    Inventors: Denis Drapeau, Yen-Tung Luan, James R. Mercer, Wenge Wang, Daniel R. Lasko
  • Patent number: 7300773
    Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.
    Type: Grant
    Filed: August 25, 2005
    Date of Patent: November 27, 2007
    Assignee: Wyeth Research Ireland Limited
    Inventors: Denis Drapeau, Yen-Tung Luan, James R. Mercer, Wenge Wang, Daniel R. Lasko