Abstract: This disclosure describes biological pesticides that include biological mannosylerythritol lipids (MELs), and their application. Provided MEL-based pesticides are microbially produced by through microbial (fungal) fermentation of plant-based derivatives or plant-derived materials as a substrate. The biologically active components are obtained from multiple stage bio-processes including transformation, biochemical reaction, extraction, and other processing of raw materials. The synthesis, separation, concentration, purification, preparation of biological pesticides, and their application as a crop pathology treatment, are described. These biological pesticides may be used disease prevention and control for crops and other plants.

Abstract: Unique carbon dioxide or lignocellulosic substrate is prepared and used to produce biosurfactants, based on different types of microorganism fermenting strains, using carbon dioxide or lignocellulose-based raw materials as the primary feedstock, subsequently utilizing a fermentation process to synthesize different structures of biosurfactants. This is a two-phase reaction where phase-one creates the feedstock for the phase-two reactions. The fermentation broth resulting from the phase-two reaction is the crude biosurfactant; it uses glycolipid or lipopeptide biosurfactant as the main component. The broth is then refined by filtration, then concentrated, and further purified to obtain the pure biosurfactant material. The biosurfactant of the present disclosure can be applied to industries such as petroleum, food or agriculture, daily chemicals, industrial chemicals, environmental protection, and medicine.

Abstract: Disclosed is a method for determining three-dimensional in-situ stress based on displacement measurement of borehole wall, including the following steps: pumping the prefluid and the sand-carrying fluid into the target interval, and injecting the biological oil displacement agent into the target interval in sequence; pumping temporary plugging agent into the target layer to implement crack plugging diverting; pumping the sand-carrying liquid into the target interval, and injecting the biological oil displacement agent into the target interval. The beneficial effect of the technical scheme proposed in this disclosure is: by injecting oil displacement agent to fracturing fluid, the temporary plugging diverting fracturing is integrated constructed with enhanced oil recovery, at the same time, it can overcome the incomplete removal of temporary plugging agent and the water lock effect of fracturing fluid caused by diverting fracturing.

Abstract: An enhanced oil recovery method includes: screening out implementation well that meet the requirements of a preset standard; selecting the target interval that can be used for enhanced oil recovery in the implementation well; obtaining the reservoir parameters and perforation parameters of the target interval, so as to divide the target interval into several groups of swallowing and exhaling segments, injecting the infection liquid containing and simmer the well for a period of time; and after the well simmering, the oil-water mixture replaced by the injection fluid is produced from each exhaling segment.

Abstract: Disclosed is a method for determining three-dimensional in-situ stress based on displacement measurement of borehole wall, including the following steps: pumping the prefluid and the sand-carrying fluid into the target interval, and injecting the biological oil displacement agent into the target interval in sequence; pumping temporary plugging agent into the target layer to implement crack plugging diverting; pumping the sand-carrying liquid into the target interval, and injecting the biological oil displacement agent into the target interval. The beneficial effect of the technical scheme proposed in this disclosure is: by injecting oil displacement agent to fracturing fluid, the temporary plugging diverting fracturing is integrated constructed with enhanced oil recovery, at the same time, it can overcome the incomplete removal of temporary plugging agent and the water lock effect of fracturing fluid caused by diverting fracturing.

Abstract: Provided herein are amphoteric glycolipid biosurfactants containing an amphoteric glycolipid biological surface active molecule preparation produced by acid precipitation of culture supernatant produced by sequentially culturing Pseudomonas, Candida, and Neurospora, for instance in a fermentation medium comprising a hydrophilic carbon source and a hydrophobic carbon source. Also described are amphoteric glycolipid biological surface active molecule preparations, and methods of making such preparations. The amphoteric molecules have anionic and cationic groups; example molecules include 17-L-[(2?-O-?-D-glucopyranosyl-?-D-Glucosyl)-O-]-octadecenoic acid amine-6?,6? diacetate, and 17-L-[(2?-O-?-D-glucopyranosamine-?-D-rhamnosyl)-O-]-octadecenoic acid-6?,6? diacetate. The amphoteric glycolipid biosurfactant has good compatibility with other types of surfactants, high temperature resistance and salt resistance, and is suitable for use in a variety of liquid systems.