Patents by Inventor Wenjin WEI

Wenjin WEI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • EV71 virus-like particles and preparation method and application thereof
    Patent number: 9782471
    Abstract: The present invention provides EV71 virus-like particles and a preparation method and application thereof. The method comprises: connecting a P1 protein gene and a 3CD protease gene of an EV71 virus with a PMV plasmid to construct a PMV-P1-3CD recombinant expression plasmid; then transforming a Hansenula polymorpha AU-0501 expression strain with the PMV-P1-3CD recombinant expression plasmid to obtain an AU-PMV-P1-3CD recombinant expression strain; fermenting and culturing the recombinant expression strain, and inducing the recombinant expression strain to express the EV71 virus-like particle protein with methanol; centrifuging and collecting mycelia for homogeneous breakage at a high pressure; and purifying the supernatant through ion-exchange chromatography, hydrophobic chromatography, and molecular sieve chromatography, so as to obtain EV71 virus-like particles.
    Type: Grant
    Filed: April 25, 2014
    Date of Patent: October 10, 2017
    Assignee: BEIJING MINHAI BIOTECHNOLOGY CO., LTD.
    Inventors: Meirong Gu, Wenjin Wei, Jiankai Liu, Linlin Song, Shanshan Xu, Guoshun Li, Lin Guo
  • EV71 VIRUS-LIKE PARTICLES AND PREPARATION METHOD AND APPLICATION THEREOF
    Publication number: 20160166675
    Abstract: The present invention provides EV71 virus-like particles and a preparation method and application thereof. The method comprises: connecting a P1 protein gene and a 3CD protease gene of an EV71 virus with a PMV plasmid to construct a PMV-P1-3CD recombinant expression plasmid; then transforming a Hansenula polymorpha AU-0501 expression strain with the PMV-P1-3CD recombinant expression plasmid to obtain an AU-PMV-P1-3CD recombinant expression strain; fermenting and culturing the recombinant expression strain, and inducing the recombinant expression strain to express the EV71 virus-like particle protein with methanol; centrifuging and collecting mycelia for homogeneous breakage at a high pressure; and purifying the supernatant through ion-exchange chromatography, hydrophobic chromatography, and molecular sieve chromatography, so as to obtain EV71 virus-like particles.
    Type: Application
    Filed: April 25, 2014
    Publication date: June 16, 2016
    Inventors: Meirong GU, Wenjin WEI, Jiankai LIU, Linlin SONG, Shanshan XU, Guoshun LI, Lin GUO