Abstract: Aiming at the problem of slow crystallization rate of poly(ethylene furandicarboxylate) (PEF), a preparation method for a bio-based nucleating agent for PEF and an application thereof are disclosed. In a technical solution, glycine having a bio-based source is subjected to an amidation reaction with furoic acid under the catalysis of p-toluenesulfonic acid, followed by an acid-base neutralization reaction with sodium hydroxide to obtain a bio-based nucleating agent for PEF. The bio-based nucleating agent prepared by the present disclosure can significantly accelerate a crystallization rate of PEF. Most importantly, the bio-based nucleating agent has no effect on the bio-based properties of PEF.