Abstract: A catalytic reaction of ethylene oxide (EO) coupling with syngas to produce 1,3-propanediol (1,3-PDO) is disclosed. The catalytic reaction of EO, carbon monoxide and the alcohol uses a N,O-ligand coordinated metal complex catalyst. The reaction is carried out in an organic solvent in the presence of an additive at the temperature of 30-190° C. and the CO pressure of 1-150 atm for 0.1-200 h to prepare 3-hydroxypropinate (3HP). The catalytic reaction of 3HP with dihydrogen uses a copper-containing mixed metal silicon oxide catalyst with a molecular formula of M?uCuvSiyOz. The reaction is carried out at 80-400° C. and 20-150 atm for 0.1-200 h to prepare the 1,3-PDO. The yield of the 1,3-PDO can reach to 73%. The alcohol byproduct generated in the second step catalytic hydrogenation reaction can be recycled to use for the first step catalytic reaction by the ring opening-carbonylation-esterification.

Abstract: Provided is a method for replicating a mirror nucleic acid, comprising: reacting a mirror nucleic acid template, a mirror nucleic acid primer and mirror dNTPs/rNTPs in the presence of a mirror nucleic acid polymerase, so as to obtain the mirror nucleic acid.

Abstract: An electric vehicle for routing inspection of power transmission lines, includes a body; a running mechanism mounted to the body, comprising a front wheel and a rear wheel connected by a cross beam and rolling on the cable; an obstacle sensor and a cam rotatably mounted to the cross beam, the cam rotating to roll onto the cable after an obstacle is detected on the cable such that the front wheel is raised to a position above the cable, and the cam continuing rolling on the cable after the front wheel passes the obstacle such that the front portion of the cross beam falls back downwards and the front wheel then falls back onto the cable, a hanging seat rotatably hanging to the body and a control device at least partially disposed in the hanging seat and comprising a braking device.

Abstract: A method of DNA fragmentation is provided. The method comprising incubating a semi-solid biological sample which comprises the DNA with an auxiliary domain-directed nuclease having a binding affinity and selectivity to pre-defined sites in the DNA so as to yield a DNA fragment-of-interest, to thereby fragment the DNA.

Abstract: An electric vehicle for routing inspection of power transmission lines, includes a body; a running mechanism mounted to the body, comprising a front wheel and a rear wheel connected by a cross beam and rolling on the cable; an obstacle sensor and a cam rotatably mounted to the cross beam, the cam rotating to roll onto the cable after an obstacle is detected on the cable such that the front wheel is raised to a position above the cable, and the cam continuing rolling on the cable after the front wheel passes the obstacle such that the front portion of the cross beam falls back downwards and the front wheel then falls back onto the cable, a hanging seat rotatably hanging to the body and a control device at least partially disposed in the hanging seat and comprising a braking device.

Abstract: A safe disposable injector with a changeable and automatically retractable needle includes a needle cylinder (1) and a plunger (2), and also includes a medicament-dispensing needle unit (3) and an injecting needle unit (4). The medicament-dispensing needle unit (3) and the injecting needle unit (4) are changed according to the requirement in use. The present invention will prevent harmful effects caused by needle deformation and variation of smoothness and sharpness during the injection to the human body. The operation of the present invention is the same as the convention injection operation. The present invention is convenient to use and reliable to operate. After injection, the injector needle can be retracted by an automatic retraction mechanism to avoid the accidental hurt to medical staff and patients by a polluted needle tip. The present invention is suitable for automatic production on the assembly line.

Abstract: A system and method for file transfer via an exclusive line is for use with at least two local area networks (LANs) connected via an exclusive line and each having at least a client and a server. The method includes: when a client sends an upload instruction, storing upload files in the server in the same LAN as the client; when a client sends a download instruction, searching the server in one of the LANs for download files specified by the download instruction, if it is not found, searching the server of the other LAN via the exclusive line and further shadow copying the found download files to the server of the other LAN via the exclusive line to allow the client of the same LAN to download, thereby providing proximate end downloading services and solving the conventional drawbacks of exclusive-line overloading, network congestion, low transmission speed and high cost.