Abstract: Provided are a chimeric antigen receptor T lymphocyte for treating tumors, a preparation method therefor, and the use thereof. The chimeric antigen receptor successively comprises a single-chain antibody against a tumor cell surface antigen, a human hinge transmembrane region, a human intracellular signal domain, a self-cleaving peptide, and the full length of human CD27. The human intracellular signal domain comprises a human intracellular co-stimulatory signal domain and a human intracellular signaling domain. The method for preparing the chimeric antigen receptor T lymphocyte comprises the following steps: introducing the coding gene of the above-mentioned chimeric antigen receptor into a T cell and expressing the coding gene, thereby obtaining the CAR-T cell.

Abstract: The present application provides an integrated droplet chip and a droplet multi-index detection method therefor and belongs to the technical field of digital PCR analyzers.

Abstract: This application provides a data processing method and a communication apparatus. The method is performed by a radio frequency unit side of a network device. The method includes: determining a first resource feature of first service data; and sending the first service data to a first processing module based on the first resource feature, where the first processing module is any one of at least one processing module, and the at least one processing module is used in a distributed unit side of the network device. In this way, a radio frequency unit of the network device performs resource feature identification of service data, and forwards corresponding service data to a processing module in a distributed unit, so that overall latency of data processing can be reduced.

Abstract: This application provides a data retransmission method and a communication apparatus. The method is performed by a radio frequency unit side of a network device. The method includes: receiving first feedback information, where the first feedback information is feedback for first downlink data of a terminal device; and when determining, based on the first feedback information, that transmission of the first downlink data fails, sending second downlink data, where the second downlink data is retransmitted data of the first downlink data. In this way, a radio frequency unit of the network device performs a processing process of data retransmission, so that overall latency of a data retransmission process can be reduced.

Abstract: The invention discloses an integrated digital PCR instrument and a control method therefor.

Abstract: The present invention provides an integrated droplet chip, including a chip body, the chip body has a reaction chamber, a sample adding chamber, droplet generating structure, oil-liquid interface, gas-liquid interface and fluorescence detection area; when the droplet is generated, a pressure difference is formed between the sampling chamber and the gas-liquid interface and between the oil-liquid interface and the gas-liquid interface, and the pressure difference respectively drives a sample in each sample adding chamber and generation oil of the oil-liquid interface to enter the droplet generating structure, so as to generate droplets, which then enter the reaction chamber; and during droplet detection, an external pressure drives detection pushing oil to enter the reaction chamber, such that the droplets flow from the reaction chamber to the droplet generating structure, the external pressure drives detection separation oil to enter the droplet generating structure, and the detection separation oil separates

Abstract: The present invention discloses an automatic potato-cutting machine, which includes a casing, a base and a push plate. The casing is arranged on the top of the base, a first installation groove is on one side of the casing, and a first buckle is arranged on one side of the first installation buckle. A shielding structure is arranged on the top of the side plate. In the present invention, a blade is movably connected inside the installation housing, and the blade and the installation housing form a snap-fit structure, so that the blade can be snapped into the inside of the installation housing, and then the first installation buckle can slide inside the first installation groove, the sliding of the first installation buckle can drive the first buckle to move, and the movement of the first buckle can be snapped into the slot on the blade.

Abstract: The invention discloses a method for purifying a retrovirus (retroviral vector), comprising the following steps: A), microfiltrating a cell culture supernatant containing a retrovirus to remove cell debris from the cell culture supernatant and obtain a micro-filtered virus solution; B), treating the micro-filtered virus solution with a nuclease to degrade host DNA residues into small DNA fragments, and obtain an enzyme-digested virus solution; C), ultrafiltering the enzyme-digested virus solution such that the retrovirus is retained in a retentate, collecting the retentate, and obtaining an ultrafiltrated virus solution; and D), performing low speed centrifugation on the ultrafiltered virus solution to precipitate the retrovirus, collecting the precipitate to obtain unsterilized virus.

Abstract: A data disaster recovery method performed by a disaster recovery site includes: selecting a first backup copy when taking over a service of a production site; receiving service data through a virtual machine; obtaining changed block tracking CBT) information based on a CBT technology and the received service data, where the CBT information includes incremental information generated after the disaster recovery site receives the first backup copy; and sending, by the disaster recovery site, a first message to the production site, where the first message includes the CBT information.

Abstract: Provided are a chimeric antigen receptor T lymphocyte for treating tumors, a preparation method therefor, and the use thereof. The chimeric antigen receptor successively comprises a single-chain antibody against a tumor cell surface antigen, a human hinge transmembrane region, a human intracellular signal domain, a self-cleaving peptide, and the full length of human CD27. The human intracellular signal domain comprises a human intracellular co-stimulatory signal domain and a human intracellular signaling domain. The method for preparing the chimeric antigen receptor T lymphocyte comprises the following steps: introducing the coding gene of the above-mentioned chimeric antigen receptor into a T cell and expressing the coding gene, thereby obtaining the CAR-T cell.

Abstract: A data disaster recovery method performed by a disaster recovery site includes selecting a first backup copy when taking over a service of a production site; receiving service data through a virtual machine; obtaining changed block tracking CBT information based on a CBT technology and the received service data, where the CBT information includes incremental information generated after the disaster recovery site receives the first backup copy; and sending, by the disaster recovery site, a first message to the production site, where the first message includes the CBT information.

Abstract: A method for issuing coupons to a plurality of target users is provided. For each of the plurality of target users, the method may include determining a predicted value of a group indicator of the target user in a predetermined period. The method may further include grouping the plurality of target users using a plurality of grouping strategies. For each of the grouping strategies, the method may further include determining a candidate coupon issuing strategy. For each of the candidate coupon issuing strategies, the method may further include obtaining user feature information of one or more target users in each group corresponding to the candidate coupon issuing strategy. For each of the candidate coupon issuing strategies, the method may further include predicting an ROI of the candidate coupon issuing strategy based on an RO prediction model and the corresponding user feature information.

Abstract: A method for querying data is provided. The method may include determining a characteristic value of a selected feature dimension among feature values of the selected feature dimension of plurality of entities and establishing a corresponding relationship between the characteristic value and the selected feature dimension. The method may also include caching the corresponding feature value into a cache memory for each entity having a feature value of the selected feature dimension being unequal to the characteristic value, and leaving the corresponding feature value without caching for each entity having a feature value of the selected feature dimension being equal to the characteristic value. The method may further include performing a first search in the cache memory to produce a first search result in response to a query request related to the plurality of entities, and generating a query result based on the corresponding relationship and the first search result.

Abstract: A method for synthesizing a polybutylene adipate terephthalate-polylactic acid (PBAT-PLA) copolyester, the method including: uniformly blending a first prepolymer PBAT and a second prepolymer PLA to yield a mixture, 0putting the mixture in a vacuum reactor with a vacuum degree of between 5 and 100 pascal, heating the vacuum reactor to a temperature of between 100 and 110° C., and allowing to react for between 0.5 and 1 hr; stepwise increasing the temperature to be between 145 and 155° C. in between 1 and 2 hrs, and allowing to react for between 5 and 10 hrs, to yield a PBAT-PLA copolyester with a weight-average molecular weight of between 100000 and 250000. The method for synthesizing the PBAT-PLA copolyester is eco-friendly, non-toxic and cost-efficient.

Abstract: A method for querying data is provided. The method may include determining a characteristic value of a selected feature dimension among feature values of the selected feature dimension of plurality of entities and establishing a corresponding relationship between the characteristic value and the selected feature dimension. The method may also include caching the corresponding feature value into a cache memory for each entity having a feature value of the selected feature dimension being unequal to the characteristic value, and leaving the corresponding feature value without caching for each entity having a feature value of the selected feature dimension being equal to the characteristic value. The method may further include performing a first search in the cache memory to produce a first search result in response to a query request related to the plurality of entities, and generating a query result based on the corresponding relationship and the first search result.

Abstract: A method for issuing coupons to a plurality of target users is provided. For each of the plurality of target users, the method may include determining a predicted value of a group indicator of the target user in a predetermined period. The method may further include grouping the plurality of target users using a plurality of grouping strategies. For each of the grouping strategies, the method may further include determining a candidate coupon issuing strategy. For each of the candidate coupon issuing strategies, the method may further include obtaining user feature information of one or more target users in each group corresponding to the candidate coupon issuing strategy. For each of the candidate coupon issuing strategies, the method may further include predicting an ROI of the candidate coupon issuing strategy based on an RO prediction model and the corresponding user feature information.

Abstract: A micro-droplet fluorescence detection system, comprising a microfluidic chip (1), an optical path device, and a movement control device for controlling the chip (1) and an optical path device to move, so that the chip (1) moves relative to the optical path device during a fluorescence scanning detection process of micro-droplets in the chip (1), and the micro-droplets inside the chip (1) remains stationary relative to the chip (1). The micro-droplet fluorescence detection system has advantages of avoiding cross contamination due to closed detection, high detection rate, high throughput and high sensitivity.

Abstract: A method for querying data is provided. The method may include determining a characteristic value of a selected feature dimension among feature values of the selected feature dimension of plurality of entities and establishing a corresponding relationship between the characteristic value and the selected feature dimension. The method may also include caching the corresponding feature value into a cache memory for each entity having a feature value of the selected feature dimension being unequal to the characteristic value, and leaving the corresponding feature value without caching for each entity having a feature value of the selected feature dimension being equal to the characteristic value. The method may further include performing a first search in the cache memory to produce a first search result in response to a query request related to the plurality of entities, and generating a query result based on the corresponding relationship and the first search result.

Abstract: A method for synthesizing a polybutylene adipate terephthalate-polylactic acid (PBAT-PLA) copolyester, the method including: uniformly blending a first prepolymer PBAT and a second prepolymer PLA to yield a mixture, putting the mixture in a vacuum reactor with a vacuum degree of between 5 and 100 pascal, heating the vacuum reactor to a temperature of between 100 and 110° C., and allowing to react for between 0.5 and 1 hr; stepwise increasing the temperature to be between 145 and 155° C. in between 1 and 2 hrs, and allowing to react for between 5 and 10 hrs, to yield a PBAT-PLA copolyester with a weight-average molecular weight of between 100000 and 250000. The method for synthesizing the PBAT-PLA copolyester is eco-friendly, non-toxic and cost-efficient.