Abstract: The present invention provides a method of manufacturing a reactive composition: (a) dissolving 2-[(4-aminophenyl)sulfonyl]ethanesulfonic acid in water to form a solution; (b) introducing excess nitrite or excess nitrous acid and the solution into an acid solution to form a diazonium salt; (c) adding 4-amino-5-hydroxy-2,7-naphthalenedisulfonic acid to react with the diazonium salt; (d) adding 7-acetamido-4-hydroxy-2-naphthalenesulfonic acid to react with the diazonium salt; (e) adding a first alkaline substance to adjust a pH value; (f) adding a second alkaline substance to adjust the pH value; (g) adding a third alkaline substance to control the pH value; (h) adding an acidic substance to adjust the pH value; and (i) adding an additive agent to obtain the reactive composition, wherein the additive agent is acetic acid, sodium hydroxide, acetate, sulfonic acid buffering agent, or a combination thereof.

Abstract: The present invention provides highly specific and selective methods for diagnosing partial lipodystrophy (e.g., familial partial lipodystrophy) in a subject. Methods for treating partial lipodystrophy are also provided.

Abstract: The present invention provides a method of manufacturing a reactive composition: (a) dissolving 2-[(4-aminophenyl)sulfonyl]ethanesulfonic acid in water to form a solution; (b) introducing excess nitrite or excess nitrous acid and the solution into an acid solution to form a diazonium salt; (c) adding 4-amino-5-hydroxy-2,7-naphthalenedisulfonic acid to react with the diazonium salt; (d) adding 7-acetamido-4-hydroxy-2-naphthalenesulfonic acid to react with the diazonium salt; (e) adding a first alkaline substance to adjust a pH value; (f) adding a second alkaline substance to adjust the pH value; (g) adding a third alkaline substance to control the pH value; (h) adding an acidic substance to adjust the pH value; and (i) adding an additive agent to obtain the reactive composition, wherein the additive agent is acetic acid, sodium hydroxide, acetate, sulfonic acid buffering agent, or a combination thereof.