Abstract: A content lock firewall method based on a white list includes performing semantic parsing on the payload of a data packet received by a website to obtain parsed texts of the received data packet, and matching the parsed texts of the data packet received by the website with a text pattern library to decide whether to forward or intercept the received data packet, the text pattern library comprising a plurality of text patterns, and each text pattern includes a sequence of keywords and a value range of each keyword. For the website with a relatively fixed function, through deployment of the firewall, known and new network attacks may be effectively defended, and the website may run with vulnerability under the condition of ensuring normal functions, without expensive upgrading.

Abstract: Disclosed are a construction method and application of a microorganism capable of realizing high production of lacto-N-neotetraose, belonging to the field of microbial genetic engineering. Coding genes of ß-1,3-acetyl glucosamine transferase, ß-1,4-galactosyl transferase and/or UDP-glucose 4 epimerase are over-expressed on the basis of a strain which is previously constructed by the team and is subjected to related-gene knockout, thus enabling the strain to have a synthesis capability of producing the lacto-N-neotetraose. The present disclosure accurately regulates the carbon flux of a metabolic pathway and relieves the metabolic stress by screening the high-efficiency ß-1,4-galactosyl transferase gene and regulating the expression of IgtA, Aa-?-1,4-GalT and galE in a lacto-N-neotetraose synthesis pathway in a combined manner. In a shake flask experiment, the lacto-N-neotetraose production capacity of Escherichia coli is 0.91 g/L. The lacto-N-neotetraose yield in a 3 L fermentation tank reaches 12.14 g/L.

Abstract: Disclosed are a construction method and application of a microorganism capable of realizing high production of lacto-N-tetrose, belonging to the field of microbial genetic engineering. The present disclosure employs a strain which has been constructed in the early stage for efficiently producing a precursor lacto-N-triose II as an original strain to synthesize a key gene of the lacto-N-tetrose via over-expression, thus enabling the strain to have a synthesis capability of producing the lacto-N-tetrose. The present disclosure improves the synthesis of the lacto-N-tetrose by screening a high-efficiency ?-1,3-galactosyl transferase gene, and reasonably designing the co-expression of the ?-1,3-galactosyl transferase gene and a key UDP-glucose 4 epimerase gene (galE) for strengthening a UDP-galactose pathway on a vector pCDFDuet-1. In a shake flask experiment, the lacto-N-tetrose production capacity of Escherichia coli is 3.04 g/L. The lacto-N-tetrose yield in a 3 L fermentation tank reaches 25.49 g/L.

Abstract: Provided are a biodegradable self-healing rubber elastomer and its preparation method, which belongs to the field of elastomeric materials. The biodegradable self-repairing rubber elastomer is prepared by a melting method in which enzymatic lignin and itaconic acid are added to lipoic acid separately, melted and blended separately, and then cooled to obtain the rubber elastomer.

Abstract: Provided are a biodegradable self-healing rubber elastomer and its preparation method, which belongs to the field of elastomeric materials. The biodegradable self-repairing rubber elastomer is prepared by a melting method in which enzymatic lignin and itaconic acid are added to lipoic acid separately, melted and blended separately, and then cooled to obtain the rubber elastomer.

Abstract: The invention relates to adenoviral vectors, cells for use in generating adenoviral vectors, methods for generating adenoviral vectors, and therapeutic uses of adenoviral vectors in gene therapy, tumour therapy and as vaccines.

Abstract: Disclosed are a construction method and application of a microorganism capable of realizing high production of lacto-N-neotetraose, belonging to the field of microbial genetic engineering. Coding genes of ?-1,3-acetyl glucosamine transferase, ?-1,4-galactosyl transferase and/or UDP-glucose 4 epimerase are over-expressed on the basis of a strain which is previously constructed by the team and is subjected to related-gene knockout, thus enabling the strain to have a synthesis capability of producing the lacto-N-neotetraose. The present disclosure accurately regulates the carbon flux of a metabolic pathway and relieves the metabolic stress by screening the high-efficiency ?-1,4-galactosyl transferase gene and regulating the expression of IgtA, Aa-?-1,4-GalT and galE in a lacto-N-neotetraose synthesis pathway in a combined manner. In a shake flask experiment, the lacto-N-neotetraose production capacity of Escherichia coli is 0.91 g/L. The lacto-N-neotetraose yield in a 3 L fermentation tank reaches 12.14 g/L.

Abstract: Disclosed are a construction method and application of a microorganism capable of realizing high production of lacto-N-tetrose, belonging to the field of microbial genetic engineering. The present disclosure employs a strain which has been constructed in the early stage for efficiently producing a precursor lacto-N-triose II as an original strain to synthesize a key gene of the lacto-N-tetrose via over-expression, thus enabling the strain to have a synthesis capability of producing the lacto-N-tetrose. The present disclosure improves the synthesis of the lacto-N-tetrose by screening a high-efficiency ?-1,3-galactosyl transferase gene, and reasonably designing the co-expression of the ?-1,3-galactosyl transferase gene and a key UDP-glucose 4 epimerase gene (galE) for strengthening a UDP-galactose pathway on a vector pCDFDuet-1. In a shake flask experiment, the lacto-N-tetrose production capacity of Escherichia coli is 3.04 g/L. The lacto-N-tetrose yield in a 3 L fermentation tank reaches 25.49 g/L.

Abstract: The invention relates to adenoviral vectors, cells for use in generating adenoviral vectors, methods for generating adenoviral vectors, and therapeutic uses of adenoviral vectors in gene therapy, tumour therapy and as vaccines.

Abstract: The present disclosure relates to the technical field of tea processing, and discloses a method for processing dried-tangerine-peel Qingzhuan tea, including: weighing tea raw materials accurately according to a weight of a dried-tangerine-peel green tea brick; mixing raw material tea leaves and dried tangerine peels evenly, and steaming a mixture with a steamer; pressing the mixture into a tea-brick mold, covering with an aluminum cover plate and a corner iron fin immediately, and pressing compactly under a pressure machine; cooling to shape a pressed tea brick, and then removing the tea brick that is cooled and shaped; feeding the removed tea bricks to a drying room, arranging the tea bricks orderly on a drying rack at regular intervals for drying treatment, stop heating after the drying treatment is finished, opening a window for cooling, taking the tea bricks out of the dry room, and then packaging.

Abstract: There is a method for forming a three dimensional or porous graphene electrode pattern on a substrate, the method including providing a substrate; coating the substrate with a lignin-polymer composite film; exposing a first part of the coated lignin-polymer composite film to a laser beam for transforming the first part into the graphene pattern; and removing a second part of the coated lignin-polymer composite film, which was not exposed to the laser beam, by placing the second part in water. The lignin-polymer composite film includes (1) a water-soluble alkaline lignin, (2) a polymer having bonding properties, and (3) a solvent, and an amount of the water-soluble alkaline lignin in the lignin-polymer composite film is between 5 and 60% by weight.

Abstract: An array substrate, a testing method and manufacturing method thereof, and a displaying device. According to the disclosure, a plurality of test capacitors connected in parallel, a first connecting wire, a second connecting wire, a first test terminal and a second test terminal are arranged in an array substrate located in a test area, each test capacitor comprises a first electrode, a first insulating layer and a second electrode which are stacked on a base, and each test capacitor has the same film structure as a storage capacitor arranged in an active area. By making the first electrode of each test capacitor in the test area connected to the first test terminal to through the first connecting wire, and the second electrode of each test capacitor connected to the second test terminal through the second connecting wire, the plurality of test capacitors are connected in parallel.

Abstract: There is a method for forming a three dimensional or porous graphene electrode pattern on a substrate, the method including providing a substrate; coating the substrate with a lignin-polymer composite film; exposing a first part of the coated lignin-polymer composite film to a laser beam for transforming the first part into the graphene pattern; and removing a second part of the coated lignin-polymer composite film, which was not exposed to the laser beam, by placing the second part in water. The lignin-polymer composite film includes (1) a water-soluble alkaline lignin, (2) a polymer having bonding properties, and (3) a solvent, and an amount of the water-soluble alkaline lignin in the lignin-polymer composite film is between 5 and 60% by weight.

Abstract: This document provides methods and materials involved in extracting chromatin. For example, methods and materials for obtaining chromatin from formalin-fixed, paraffin-embedded (FFPE) tissues are provided.

Abstract: The invention relates to a sliding member having a first sliding surface, wherein the first sliding surface (29) comprises a particulate support material (6) and a graphene-containing material (7), wherein the particulate support material (6) is at least partially coated with the graphene-containing material (7), and wherein a material bond (14) is present between the particulate support material (6) and the graphene-containing material (7).

Abstract: A packaged electronic device comprises a die attach pad enclosed by a package structure, a semiconductor die mounted to a side of the die attach pad, a conductive plate and a polymer layer having a first side on a side of the conductive plate and a second side on the die attach pad. A method of manufacturing a packaged electronic device comprises attaching a first side of a polymer layer to a first side of a conductive plate, attaching a first side of a die attach pad to a second side of the polymer layer and attaching a first side of a semiconductor die to a second side of the die attach pad.

Abstract: The invention relates to a sliding member having a first sliding surface, wherein the first sliding surface (29) comprises a particulate support material (6) and a graphene-containing material (7), wherein the particulate support material (6) is at least partially coated with the graphene-containing material (7), and wherein a material bond (14) is present between the particulate support material (6) and the graphene-containing material (7).

Abstract: There is a method for forming a three dimensional or porous graphene electrode pattern on a substrate, the method including providing a substrate; coating the substrate with a lignin-polymer composite film; exposing a first part of the coated lignin-polymer composite film to a laser beam for transforming the first part into the graphene pattern; and removing a second part of the coated lignin-polymer composite film, which was not exposed to the laser beam, by placing the second part in water. The lignin-polymer composite film includes (1) a water-soluble alkaline lignin, (2) a polymer having bonding properties, and (3) a solvent, and an amount of the water-soluble alkaline lignin in the lignin-polymer composite film is between 5 and 60% by weight.

Abstract: This document provides methods and materials involved in extracting chromatin. For example, methods and materials for obtaining chromatin from formalin-fixed, paraffin-embedded (FFPE) tissues are provided.

Abstract: A system includes reception of an instruction to determine configuration properties of an instance of a first business object, determination of a first type of the instance of the first business object, determination of whether or not the first type is extendable. In a case it is determined that the first type is extendable, a second reference object is determined, the second reference object conforming to the first type, an instance of the second reference object is determined, configuration properties of the instance of the second reference object are determined, and, in response to the instruction, at least the configuration properties of the instance of the second reference object are returned as the configuration properties of the first instance of the first business object.