Abstract: The invention discloses a construction method of genetic engineering fungi of filamentous fungi. Through the genetic engineering method, the filamentous fungi overexpress the positive regulation genes of ethanol synthesis, and/or down regulate the negative regulation genes of endogenous ethanol synthesis to obtain genetic engineering strains. Or overexpression of acetaldehyde dehydrogenase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of pyruvate decarboxylase and ethanol dehydrogenase containing mitochondrial localization signal sequence, or overexpression of acetaldehyde dehydrogenase, ethanol dehydrogenase and pyruvate decarboxylase containing mitochondrial localization signal sequence in filamentous fungal cells. Compared with the original strain, the ethanol synthesis ability of the obtained genetically engineered strains are improved.

Abstract: Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved.

Abstract: A MEMS microphone and a manufacturing method thereof. The method comprises: sequentially forming a first isolation layer, a diaphragm, and a second isolation layer on a substrate; sequentially forming a first protective layer, a backplate electrode, and a second protective layer on the second isolation layer; forming a release hole penetrating through the first protective layer, the backplate electrode, and the second protective layer; forming an acoustic cavity penetrating through the substrate; releasing the diaphragm through the acoustic cavity and the release hole; and forming a groove on a surface of the first isolation layer, wherein the diaphragm conformally covers the surface of the first isolation layer, thereby forming a spring structure at a position of the groove.

Abstract: A MEMS microphone and a manufacturing method thereof. The method comprises: sequentially forming a first isolation layer, a diaphragm, and a second isolation layer on a substrate; sequentially forming a first protective layer, a backplate electrode, and a second protective layer on the second isolation layer; forming a release hole penetrating through the first protective layer, the backplate electrode, and the second protective layer; forming an acoustic cavity penetrating through the substrate; releasing the diaphragm through the acoustic cavity and the release hole; and forming a groove on a surface of the first isolation layer, wherein the diaphragm conformally covers the surface of the first isolation layer, thereby forming a spring structure at a position of the groove.

Abstract: Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved.

Abstract: Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved.

Abstract: Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved.

Abstract: A supercritical extraction of characteristic fragrant substances in different fragrant tobacco leaves, which involves the following steps: 1) cutting the tobacco leaves into shreds and placing the shreds into an extraction vessel with supercritical CO2 fluid; 2) setting the extraction pressure and extraction temperature of supercritical CO2 fluid about 100 to 350 bar and 35 to 70° C., extracting statically the tobacco shreds for 10 to 35 minutes in the condition, then setting the dynamic extracting speed of the supercritical CO2 fluid about 1.0 to 3.5 L/min, and extracting dynamically the tobacco shreds for 5 to 15 minutes; 3) collecting the obtained supercritical CO2 fluid extract, and obtaining the key fragrant substances, which act as the determinants for tobacco fragrance, after completely volatilizing CO2.