Abstract: The present invention provides a method for single-cell analysis, comprising dividing a cell and a bead attached with multiple barcoded oligonucleotides into a partition. Each of the multiple barcoded oligonucleotides may contain a cell barcode and a unique molecular identifier (UMI), and binds to a poly A sequence of mRNA by means of a polyT sequence at the end of the oligonucleotide to complete the capture of mRNA. First-strand and second-strand synthesis of mRNA are completed by reverse transcription and PCR amplification, respectively. One part of cDNA is used to construct a transcriptome sequencing library, and the other part of cDNA is circularized to form circular double-stranded cDNA, which is used as a template to specifically enrich a target gene.

Abstract: A guide and flattening mechanism includes a mounting seat having a support plane and an elastic guide assembly arranged at the mounting seat and having an inclined surface. The inclined surface is arranged around a central axis perpendicular to the support plane and is located on a side of the elastic guide assembly facing away from the support plane. From a side of the inclined surface close to the central axis to a side of the inclined surface away from the central axis, at least a part of the inclined surface is arranged obliquely in a direction away from the support plane. The inclined surface is configured to be elastically deformed in a direction approaching the support plane when being squeezed, so as to guide the bending of the to-be-flattened member and flatten the to-be-flattened member on the circular end surface with the cooperation of the support plane.

Abstract: Provided are a connecting component, a battery cell, a battery, and an electric apparatus. The connecting component is configured to connect an electrode terminal and electrode assembly of the battery cell. The connecting component is provided with a stress release structure, and the stress release structure is configured to release stress at a weld between the connecting component and the electrode terminal. In the foregoing manner, this application can solve the problems of cracking and detachment in the welding structure between the connecting component and the electrode terminal, improving stability of welding.

Abstract: Provided are a tab coating device. The tab coating device is configured to cover the insulating film on the end face of the tab of the tab coating device. It comprises a sleeve and a shaping piece. The sleeve is configured to be arranged on the outer peripheral surface of the electrode assembly in a sleeving mode. The shaping piece is configured to move along the axial direction of the sleeve and extend into the sleeve, to flatten the part of the insulating film that exceeds the end face of the tab onto the end face of the tab.

Abstract: A battery cell includes a housing having an opening, an end cap assembly for closing the opening and including an end cap body and an electrode terminal connected to the end cap body in an insulating manner, and an electrode assembly arranged in the housing and being of a wound structure and including: a wound main body, a first tab and a second tab, the first tab and the second tab having opposite polarities and being connected to the same side of the wound main body along a winding axis of the wound structure. The first tab is electrically connected to the electrode terminal, and the second tab is electrically connected to the end cap body.

Abstract: A necking and flanging apparatus includes: an inner mold; a necking piece, disposed around the inner mold, where the necking piece is movable along a radial direction of the inner mold, and the necking piece is configured to coordinate with the inner mold to form a necked portion on a housing; and a press-fit piece, disposed around the inner mold, where the press-fit piece is movable along an axial direction of the inner mold, and the press-fit piece is configured to fit the necking piece to press and flatten the flanged portion of the housing.

Abstract: A battery cell includes a housing, an electrode component, an end cap, an electrode terminal, and an insulating component. The housing has an opening. The electrode component is accommodated within the housing. The end cap is configured to cover the opening. The electrode terminal is disposed on the end cap and electrically connected to the electrode component. The insulating component is configured to insulate and separate the end cap from the electrode terminal, and at least a part of the insulating component is located between the end cap and the electrode terminal and is attached to the end cap and the electrode terminal.

Abstract: A battery cell includes a housing, an end cap, an electrode assembly, and an insulator. An opening is created on the housing. The end cap is configured to fit and cover the opening. The electrode assembly is accommodated in the housing and includes a body portion, a first tab, and a second tab. The first tab and the second tab are of opposite polarities and located on a same side of the body portion. A least a part of the insulator is disposed between the first tab and the second tab and configured to restrict the first tab from deforming toward the second tab.

Abstract: The present disclosure provides methods, compositions, and kits for high-throughput single-cell analysis, such as nucleic acid analysis. In some embodiments, methods, compositions and kits for indexing a plurality of samples are provided. In some embodiments, methods, compositions and kits for high-throughput single-cell nucleic acid analysis, such as RNA sequencing are provided. The present disclosure allows fast, efficient and convenient cell labeling and sample pooling without being limited by cell types.

Abstract: A battery cell, a battery, an electrical device, and method and equipment for manufacturing the battery cell are provided. The battery cell includes a housing, an electrode assembly, an end cap, and an electrode terminal. An opening is created on the housing. The electrode assembly is accommodated in the housing. The electrode assembly includes a body portion, a first tab, and a second tab. The first tab and the second tab are of opposite polarities and located on a same side of the body portion. The end cap is configured to fit and cover the opening and be electrically connected to the first tab. The electrode terminal is dielectrically connected to the end cap. The electrode terminal includes a first recess. The electrode terminal includes a first connecting portion. In a thickness direction of the end cap, the first connecting portion is located between the first recess and the second tab.

Abstract: A battery cell includes a housing, an end cap, an electrode terminal, an electrode assembly, and an insulating member. The housing has an opening. The end cap is provided for covering the opening. The electrode terminal is in insulated connection with the end cap. The electrode assembly is housed within the housing and includes a main body and first and second tabs. The first and second tab have different polarities and are disposed on a same end of the main body facing the end cap. One of the first and second tabs is provided for electrically connecting the end cap, and the other one is provided for electrically connecting the electrode terminal. The insulating member is at least partially located between the first tab and the second tab, and abuts against the main body along a thickness direction of the end cap.

Abstract: A method of applying a dielectric coating on a structure array of a component of an electrical device includes applying a first layer of a first dielectric material on a structure array with an atomic layer deposition (ALD) process. The structure array is formed on a substrate and has a plurality of features, each of the plurality of features having an aspect ratio of at least 1:1.

Abstract: A battery cell, a manufacturing method and manufacturing device thereof, a battery, and an electric apparatus are described. The battery cell includes a housing assembly, an electrode assembly, and a current collecting member. The housing assembly includes an electrode lead-out portion for inputting or outputting electrical energy. The electrode assembly is provided in the housing assembly, and the electrode assembly has a tab. The current collecting member is provided in the housing assembly, and the current collecting member is configured to electrically connect the tab and the electrode lead-out portion. The current collecting member includes a body and an elastic portion connected to the body, one of the body and the elastic portion is welded to the tab, and the other is electrically connected to the electrode lead-out portion, and the elastic portion is configured to cushion vibrations between the electrode assembly and the housing assembly.

Abstract: Provided are a battery cell, a battery, an electrical device, and a manufacturing method and device for a battery cell, which belong to the field of battery technologies. The battery cell includes a case, an electrode assembly, an end cover, and a current collecting member. The case has an opening. The electrode assembly is accommodated in the case. The end cover covers the opening and is sealed and connected to the case. The current collecting member is accommodated in the case and located at a side of the electrode assembly facing the end cover. The current collecting member is configured to connect the case and the electrode assembly to electrically connect the electrode assembly to the case. The battery cell with such structure realizes an electrical connection between the electrode assembly and the case through the current collecting member. During assembling of the battery cell.

Abstract: A battery cell, a method and system for manufacturing the battery cell, a battery, and a power consuming device are disclosed. The battery cell includes: a shell including an electrode draw-out portion; and an electrode assembly accommodated in the shell. The end of the electrode assembly facing toward the electrode draw-out portion is provided with a first tab, the first tab is wound around a winding axis of the electrode assembly and includes a plurality of turns of tab layers, and at least some of the plurality of turns of tab layers are welded to form a first welded portion. Some of the plurality of turns of tab layers are welded to the electrode draw-out portion to form a second welded portion; and among the tab layers connected to the first welded portion, at least one turn of tab layer is not welded to the electrode draw-out portion.

Abstract: The present disclosure provides methods, reagents, compositions, systems and kits for high-throughput drug screening by using barcode molecules. In some embodiments, the method uses one step RT-PCR to simplify the experimental procedure and avoid RNA contamination in the experimental process.

Abstract: A battery cell, a battery, an electrical device, and a device and method for manufacturing a battery cell are described. The battery cell includes a shell, an electrode assembly, and a current collecting member, where the electrode assembly is accommodated in the shell, and the electrode assembly is provided with a tab; the current collecting member includes a first connecting part and a second connecting part which are connected, the first connecting part is used to be welded to the tab, and the second connecting part is used to be welded to the shell; and the first connecting part and the second connecting part are made of different materials, the first connecting part and the tab are made of the same material, and the second connecting part and the shell are made of the same material.

Abstract: High-performance optical-metasurface-based components configured to at frequencies of UV light and, in particular, in deep UV range and performing multiple optical-wavefront-shaping functions (among which there are high-numerical-aperture lensing, accelerating beam generation, and hologram projection). As a representative material for such components, hafnium oxide demands creation and establishment of a novel process of manufacture that is nevertheless based on general principles of Damascene lithography, to be compatible with existing technology and yet sufficient for producing high-aspect-ratio features that currently-used materials and processes simply do not deliver. The described invention opens a way towards low-form-factor, multifunctional ultraviolet nanophotonic platforms based on flat optical components and enabling diverse applications including lithography, imaging, spectroscopy, and quantum information processing.

Abstract: This application discloses a battery cell, a battery, an electrical device, and a method and equipment for manufacturing a battery cell. The battery cell includes a housing, an end cap, and an electrode terminal. An opening is made on the housing. The end cap covers the opening and is seamed to the housing. The electrode terminal is disposed on the end cap. A reinforcing portion is disposed on the end cap. A projection of the electrode terminal on the end cap does not overlap the reinforcing portion. The technical solution provided herein can solve a battery safety problem caused by an increased internal pressure during the use of the battery, and improve the lifespan of the battery.

Abstract: A chiral light-field optical imaging camera incorporating an array of photonic spin-multiplexed bifocal metalenses. Combined with a novel deep learning-based neural network reconstruction algorithm, the system provides distinct aberration-free photographic capabilities, including the ability to achieve a polarization controllable extremely large depth-of-field optical imaging across a range of distances within a range covering at least five orders of magnitude in absence of moving a part of the camera and based on a single exposure of an optical detector while maintaining high spatial lateral resolution.