Abstract: A hybridoma cell line secreting an anti-polystyrene monoclonal antibody and use thereof are provided. The hybridoma cell line includes hybridoma cell line PS-8 and hybridoma cell line PS-17 which are deposited with the China Center for Type Culture Collection under the Accession numbers of CCTCC NO: C2024144 and CCTCC NO: C2024154. The passage cell line can stably secrete the anti-polystyrene monoclonal antibody. The secreted antibody is high in potency, good in specificity and high in sensitivity, can be applied to kit detection, and has potential application values.

Abstract: A hybridoma cell line secreting an anti-micro/nano plastic monoclonal antibody and use thereof are provided. The hybridoma cell line includes hybridoma cell line MP-4 deposited under the Accession number of CCTCC NO: C2024262 and hybridoma cell line MP-9 under the Accession number of CCTCC NO: C2024261, which are screened by the inventor. The passage cell lines of the hybridoma cells can stably secrete the anti-broad-spectrum micro/nano plastic protein monoclonal antibody. The secreted antibody is high in potency and good in sensitivity, and can simultaneously recognize polystyrene (PS), polyvinyl chloride (PVC), polypropylene (PP), polyethylene (PE), and polyethylene terephthalate (PET), with good broad-spectrum recognition. Therefore, the hybridoma cell line provides a core raw material for establishing broad immune detection methods of microplastics and has wide application prospects.

Abstract: The present disclosure belongs to the technical field of biosensors and particularly provides a construction method for a photocathode indirect competition sensor and an evaluation method. The construction method includes: using Z-type Bi2O3/CuBi2O4 as a sensing platform; calculating a photoinduced electron Z-type transfer path and an energy band structure of Bi2O3 and CuBi2O4 using a density functional theory (DFT); and constructing a Bi2O3/CuBi2O4-based biosensor. A photoelectrochemical (PEC) photocathode biosensor based on a Bi2O3/CuBi2O4 heterojunction prepared through the solution has good repeatability, reproducibility, stability, and specificity for detecting a target. The PEC biosensor constructed in the solution of the present disclosure has a broad application prospect in the fields of healthcare, environment, and food.

Abstract: The present disclosure belongs to the technical field of biosensors and particularly provides a construction method for a photocathode indirect competition sensor and an evaluation method. The construction method includes: using Z-type Bi2O3/CuBi2O4 as a sensing platform; calculating a photoinduced electron Z-type transfer path and an energy band structure of Bi2O3 and CuBi2O4 using a density functional theory (DFT); and constructing a Bi2O3/CuBi2O4-based biosensor. A photoelectrochemical (PEC) photocathode biosensor based on a Bi2O3/CuBi2O4 heterojunction prepared through the solution has good repeatability, reproducibility, stability, and specificity for detecting a target. The PEC biosensor constructed in the solution of the present disclosure has a broad application prospect in the fields of healthcare, environment, and food.

Abstract: A method and system for implementing verification within data transfer are provided. The method includes obtaining a user card identifier and a data transfer password of a selected user card through a data transfer request interface. A data transfer verification request to a data transfer server is initiated, the data transfer verification request being generated according to the user card identifier, the data transfer password and a data transfer user identifier. A current time valid identifier that is returned by the data transfer server is received when the data transfer verification is passed, the current time valid identifier being issued when the data transfer server determines that the current time does not exceed a valid period of card-binding time of the selected user card. A secondary verification interface is skipped according to the current time valid identifier. A data transfer confirmation interface is entered.

Abstract: A method and system for implementing verification within data transfer are provided. The method includes obtaining a user card identifier and a data transfer password of a selected user card through a data transfer request interface. A data transfer verification request to a data transfer server is initiated, the data transfer verification request being generated according to the user card identifier, the data transfer password and a data transfer user identifier. A current time valid identifier that is returned by the data transfer server is received when the data transfer verification is passed, the current time valid identifier being issued when the data transfer server determines that the current time does not exceed a valid period of card-binding time of the selected user card. A secondary verification interface is skipped according to the current time valid identifier. A data transfer confirmation interface is entered.