Abstract: Methods and applications of a lyophilized tissue matrix derived powder (TH Powder) are disclosed. The methods include improved approaches to viral and bacterial control, improved multi-stage grinding techniques and use of improved grinding systems. The TH powder produced using the disclosed methods includes properties, such as small powder particle sizes, nearly instant rehydration time, high solubility, flowability and injectability, and optionally short (˜3-5 minute) polymerization time duration, that enable or improve important clinical applications. A variety of clinical applications are disclosed, including, for example, some that take advantage of the TH Powder's effectiveness in optimizing wound healing, providing a 3D scaffold onto the wound site, improving cells regeneration and proliferation, providing a temporary coverage to avoid irritations, anti-fibrosis, anti-adhesion and anti-inflammation, The TH powder is optionally sterilized while maintaining biological components.