Abstract: The disclosure provides a kit for detecting microsatellite instability and a method therefor. The kit includes a negative control, a plurality of qPCR reaction solutions, a qPCR premix and a sterile enzyme-free water; the plurality of qPCR reaction solutions includes 6 pairs of upstream primers and downstream primers of which the MSI mutation site is amplified, and a reference probe for the internal reference and a detection probe for the mutation site. The difference between the amplification of the gene and the gene at the mutation site of the samples and the negative control is used to detect the microsatellite instability. The method and kit as provided is easy and simple without the need of normal tissues being a control, and the need to open the cap. By doing so, aerosol pollution is avoided and sample supplies are conserved.

Abstract: A feeder automation system and a method for operating the feeder automation system are disclosed. The feeder automation system comprises a first domain comprising a first slave station; a second domain comprising a second slave station; and a tie-switch provided between the first domain and the second domain. The first slave station is adapted to operate the tie-switch directly, and the second slave station is adapted to operate the tie-switch through the first slave station. When a fault occurs in the system, the first slave station and the second slave station cooperate with each other to perform fault detection, fault isolation, and fault restoration etc. without interacting with a master station.