Abstract: The invention relates to a recombinant Escherichia coli expressing a fusion protein of formamidase and phosphite dehydrogenase, a construction method and use thereof. The invention includes adopting engineered E. coli DH5? as a host, amplifying a cloned formamidase gene and a cloned phosphite dehydrogenase gene into a fusion gene, ligating the fusion gene to a multiple cloning site of a vector, transforming the obtained recombinant plasmid into the E. coli DH5?, extracting the plasmid and transforming into an expression strain, and performing induction culture to obtain a recombinant E. coli. The recombinant E. coli can express a fusion protein of formamidase and phosphite dehydrogenase.

Abstract: The present invention provides a Dendrobium officinale (D. officinale) endophytic fungus strain and an extracellular polysaccharide produced thereby as well as an extraction method and application of the extracellular polysaccharide. The extraction process of the extracellular polysaccharide includes: activating a strain; culturing seed liquid; fermenting and culturing; filtering the fermented liquid under vacuum; concentrated the filtrate under vacuum; conducting alcohol precipitation on the concentrated liquid; precipitating and centrifuging; dialyzing after dissolving the precipitates in water; freezing and drying the dialysate under vacuum; and obtaining the extracellular polysaccharide. The D. officinale endophytic fungus strain belongs to Fusarium solani, and the extracellular polysaccharide produced thereby has inhibitory effects on Escherichia coli, Staphylococcus aureus, Salmonella ssp., and Bacillus subtilis.

Abstract: Disclosed is a method for producing special oil OPO by way of microbial fermentation. The method comprises the following steps: inoculating microorganisms to an activating culture medium for shake cultivation treatment to obtain an activated culture; adding an organic matter into a minimal medium to obtain an optimized culture medium; inoculating the culture into the optimized culture medium for shake cultivation treatment to obtain a culture solution; carrying out centrifugal treatment on the culture solution, taking a precipitate, washing the precipitate and freeze-drying the precipitate, adding a hydrochloric acid solution to obtain a mixture; and adding an organic solvent into the mixture for extraction, and removing the solvent to obtain the OPO, wherein the microorganisms are Rhodococcus opacus PD630.

Abstract: The invention relates to a recombinant Escherichia coli expressing a fusion protein of formamidase and phosphite dehydrogenase, a construction method and use thereof. The invention includes adopting engineered E. coli DH5? as a host, amplifying a cloned formamidase gene and a cloned phosphite dehydrogenase gene into a fusion gene, ligating the fusion gene to a multiple cloning site of a vector, transforming the obtained recombinant plasmid into the E. coli DH5?, extracting the plasmid and transforming into an expression strain, and performing induction culture to obtain a recombinant E. coli. The recombinant E. coli can express a fusion protein of formamidase and phosphite dehydrogenase.

Abstract: The present invention provides a Dendrobium officinale (D. officinale) endophytic fungus strain and an extracellular polysaccharide produced thereby as well as an extraction method and application of the extracellular polysaccharide. The extraction process of the extracellular polysaccharide includes: activating a strain; culturing seed liquid; fermenting and culturing; filtering the fermented liquid under vacuum; concentrated the filtrate under vacuum; conducting alcohol precipitation on the concentrated liquid; precipitating and centrifuging; dialyzing after dissolving the precipitates in water; freezing and drying the dialysate under vacuum; and obtaining the extracellular polysaccharide. The D. officinale endophytic fungus strain belongs to Fusarium solani, and the extracellular polysaccharide produced thereby has inhibitory effects on Escherichia coli, Staphylococcus aureus, Salmonella ssp., and Bacillus subtilis.