Abstract: The present invention relates to a method of producing a polypeptide having ?-hexosaminidase activity, comprising the steps of a) providing a yeast cell comprising a polynucleotide encoding a polypeptide having ?-hexosaminidase activity and having an amino acid sequence being at least 95% identical to the amino acid sequence shown in SEQ ID NO: 1, b) cultivating said yeast cell under conditions which allow for the production of the polypeptide, and c) obtaining the polypeptide produced in step b). The present invention further concerns a polynucleotide encoding a polypeptide having ?-hexosaminidase activity and having an amino acid sequence being at least 95% identical to the amino acid sequence shown in SEQ ID NO: 1, as well as polypeptide encoded by said polynucleotide. Moreover, the present invention concerns a yeast cell comprising the polynucleotide of the present invention.

Abstract: A coated substrate is described including a substrate material, which is coated at least in part with an oxidation-resistant coating, wherein the coating consists of a wear-resistant abrasive coating layer, which contains or consists of coated abrasive particles embedded in an oxidation-resistant matrix material, wherein at least some of the abrasive particles consist of ?-Al2O3 and the abrasive particles are coated with a first particle coating layer disposed on the abrasive particles and an optional second particle coating layer disposed on the first particle coating layer, wherein the matrix material contains or consists of the compound MCrAlY, wherein M is at least one element selected from the group consisting of Ni, Co and Fe. A method for manufacturing such a coated substrate is also disclosed.

Abstract: The invention relates to a method for welding rotors for power generation having a plurality of rotor discs arranged along a rotor axis. The method includes providing forged and NDT tested rotor discs and machining the discs for weld seam preparation. The weld seam preparation has an inner narrow TIG welding gap and an adjoining outer SAW welding gap. The method further includes stapling the discs on top of each other; and checking the run-out of the stapled discs relative to each other and as a whole and, if necessary, adjusting the staple. In addition the method includes melting the root of the weld without weld filler using TIG welding; increasing the weld height by narrow gap TIG welding with parent metal weld filler to allow tilting of the rotor in horizontal position; tilting the rotor in horizontal position; finalizing the welding by filling the outer SAW welding gap using SAW welding; and checking the welds of the rotor by NDT using ultrasonic testing.

Abstract: The invention relates to a method for welding rotors for power generation such as gas turbines, steam turbines, and generators having a plurality of rotor discs arranged along a rotor axis. The method includes providing said discs with a weld seam preparation. The weld seam preparation includes a root, a first, gap-like welding section adjoining the root in radial direction, and a second, gap-like welding section adjoining the first welding section in radial direction. The method further includes melting the root by means of a first welding process; making a weld in the first welding section by means of a second welding process; and filling up the second welding section by means of a third welding process.

Abstract: A coated substrate is described including a substrate material, which is coated at least in part with an oxidation-resistant coating, wherein the coating consists of a wear-resistant abrasive coating layer, which contains or consists of coated abrasive particles embedded in an oxidation-resistant matrix material, wherein at least some of the abrasive particles consist of ?-Al2O3 and the abrasive particles are coated with a first particle coating layer disposed on the abrasive particles and an optional second particle coating layer disposed on the first particle coating layer, wherein the matrix material contains or consists of the compound MCrAlY, wherein M is at least one element selected from the group consisting of Ni, Co and Fe. A method for manufacturing such a coated substrate is also disclosed.

Abstract: The invention relates to a method for welding rotors for power generation such as gas turbines, steam turbines, and generators having a plurality of rotor discs arranged along a rotor axis. The method includes providing said discs with a weld seam preparation. The weld seam preparation includes a root, a first, gap-like welding section adjoining the root in radial direction, and a second, gap-like welding section adjoining the first welding section in radial direction. The method further includes melting the root by means of a first welding process; making a weld in the first welding section by means of a second welding process; and filling up the second welding section by means of a third welding process.

Abstract: The invention relates to a method for welding rotors for power generation having a plurality of rotor discs arranged along a rotor axis. The method includes providing forged and NDT tested rotor discs and machining the discs for weld seam preparation. The weld seam preparation has an inner narrow TIG welding gap and an adjoining outer SAW welding gap. The method further includes stapling the discs on top of each other; and checking the run-out of the stapled discs relative to each other and as a whole and, if necessary, adjusting the staple. In addition the method includes melting the root of the weld without weld filler using TIG welding; increasing the weld height by narrow gap TIG welding with parent metal weld filler to allow tilting of the rotor in horizontal position; tilting the rotor in horizontal position; finalizing the welding by filling the outer SAW welding gap using SAW welding; and checking the welds of the rotor by NDT using ultrasonic testing.

Abstract: A method for producing oil and/or gas from an underground formation comprising locating a suitable reservoir in a subsurface formation; creating a model of the reservoir; populating the model with laboratory data; modeling the reservoir to determine fluid displacements based on fluids injected and fluids produced; determining an optimum fluid mixture for the fluids to be injected based on a series of sensitivity analyses performed with the model; drilling a first well in the formation; injecting the optimum fluid mixture into the first well; drilling a second well in the formation; and producing oil and/or gas from the second well.

Abstract: Packaging is disclosed for electrical installation equipment, in particular, made from a folded cardboard box, with two parallel first cardboard portions which form a front and a rear wall of the packaging and with two second cardboard faces extending perpendicular thereto, of which the cardboard face forms the bottom wall and the cardboard face forms the upper wall, with first flaps formed on the longitudinal edges of the front and rear wall and the bottom wall, which are attached to the longitudinal edges and able to be bent towards one another, so that they are in a single plane, with cover flaps attached to the edges of the upper wall extending in line with the longitudinal edges and which cover the first flaps and are bonded thereto, and with a third flap arranged on the upper wall which terminates on the edge of the upper wall opposing the front wall and is bonded from inside against the inner face of the front wall.

Abstract: Packaging is disclosed for electrical installation equipment, in particular, made from a folded cardboard box, with two parallel first cardboard portions which form a front and a rear wall of the packaging and with two second cardboard faces extending perpendicular thereto, of which the cardboard face forms the bottom wall and the cardboard face forms the upper wall, with first flaps formed on the longitudinal edges of the front and rear wall and the bottom wall, which are attached to the longitudinal edges and able to be bent towards one another, so that they are in a single plane, with cover flaps attached to the edges of the upper wall extending in line with the longitudinal edges and which cover the first flaps and are bonded thereto, and with a third flap arranged on the upper wall which terminates on the edge of the upper wall opposing the front wall and is bonded from inside against the inner face of the front wall.

Abstract: A volume-adjusting insert for sample vessels comprises an elongated body with a septum seal on one end and a seal surface on the outside of the body. A through-chamber in the body provides a receiving chamber for a hypodermic needle inserted into the septum. The seal surface of the insert defines a reduced-volume sample chamber comprising the through-chamber and the vessel chamber portion of the sample vessel below the insert, or alternatively, below the seal surface. Sample fluid injected into, or withdrawn from, the insert communicates with the through-chamber and the lower vessel chamber. The device is particularly useful in converting multi-well microplates to low sample-volume vessels for automated sampling and testing, and allows the hydraulic pressure generated by a hypodermic needle to transport the sample fluid through the sample vessel and out of a bottom-extraction element.

Abstract: A telescopic boom (1) for a vehicle (25) or a hoist with a storage rack (6) for at least two box girders (2, 3) guided displaceably in one another in the direction of their longitudinal axes, which are mounted to pivot about a horizontal pivot axle (5) in the storage rack (6) and which can be displaced reciprocally by means of a servo-drive (19) is described. In order to create advantageous structural conditions it is proposed that the longitudinal axles of the box girders (2, 3) form an upwards arched arc of a circle (4) which runs concentrically to a common axis parallel to the pivot axis (5).

Abstract: A container formed from an integral blank with a configuration having a top section and a bottom section attached to each other by a first line of weakness such that upon the application of sufficient pressure, the first line of weakness breaks the attachment of the top section from the bottom section. Upon breaking the attachment, a cover is formed from the top section that is hingedly attached to the bottom section at the rear wall panel. A releasable locking device having a first locking member on the cover is attached by a second line of weakness to a second locking member on the front wall portion. The first locking member and second locking member is in an aligned relationship such that when the second line of weakness is broken, the cover pivotally moves to the open position and a recess is formed between the front panel and side panels and the inner body portion.

Abstract: Self-washing injection apparatus [101] for liquid chromatography injection valves utilizes an interior chamber [105] with a penetrable seal [109] at the top end of the chamber and a needle sleeve seal [113] at the bottom end of the interior chamber. A wash port [145] in the body [103] of the apparatus connects one or more sources of wash fluid to the interior chamber between the penetrable seal and the sleeve seal. The apparatus provides a simple and effective means to aspirate or pump wash fluids from one or more wash fluid reservoirs through the apparatus, the injection port of the injection valve, and associated components.

Abstract: Self-washing injection apparatus [101] for liquid chromatography injection valves utilizes an interior chamber [105] with a penetrable seal [109] at the top end of the chamber and a needle sleeve seal [113] at the bottom end of the interior chamber. A wash port [145] in the body [103] of the apparatus connects one or more sources of wash fluid to the interior chamber between the penetrable seal and the sleeve seal. The apparatus provides a simple and effective means to aspirate or pump wash fluids from one or more wash fluid reservoirs through the apparatus, the injection port of the injection valve, and associated components.

Abstract: A loading device for containers (C) comprises a pair of loading units (2), each having a telescopic column (4) supported on a bracket (3) so as to be swivelled about a common swivel axis (I). The telescopic column consists of a lower portion (9), an upper portion (11) and a column cylinder (17) moving the upper portion (11) with respect to the lower portion (9). Actuating cylinders (5) are connected to the lower portion (9) on both sides, and the upper portion (11) accommodates an extension arm (14) which can be swivelled about a swivel axis (III) parallel to the swivel axis (I) of the telescopic column. To expand the loading possibilities, the upper portion (11) carries a column head (12) which forms a slot-shaped swivel guide (18) with cam tracks (19) limiting the swivelling of the extension arm (14).

Abstract: A pneumatic hammer comprises a rigid unit of construction, which comprises a housing, a handle, an operating cylinder resiliently supported in the housing, and a tool holder for receiving a tool movable in a striking direction by the operating cylinder. The operating cylinder is axially movable in the housing relative to the tool holder. Compressed air is supplied to the operating cylinder, and a damping spring acts upon the tool holder in a direction opposite to the striking direction.

Abstract: A loading device for containers comprises a pair of like loading units to be mounted on a vehicle frame having a longitudinally extending center axis.

Abstract: A surgical instrument capable of being used to guide a thread inside a body and to cut the thread comprises a forceps jaw having first and second opposed surfaces each provided with a serrated portion and a cutting portion.

Abstract: An instrument for holding and chipping a bond has a pair of elongated curved handles spaced apart at one end, and pivotally connected at other ends to ends of flat elongated jaws. Central portions of the jaws are pivotally connected together. Outer ends of the jaws and handles normally flare outwardly and are held by outwardly biased leaf springs secured to the handles. The handles pivot on a free floating disk disposed in slots formed in facting edges of the handles. The outer ends of the jaws are formed with interfitting teeth and recessed having side walls formed with sharp cutting edges.