Abstract: The present invention relates to a method for releasing a ligand from a complex of the ligand with an endogenous protein using a releasing agent.

Abstract: One aspect of the present invention relates to a method for releasing a ligand from a complex thereof. The method comprises contacting a medium suspected of containing such complex with an effective amount of a compound effective in releasing the ligand. Another aspect of the present invention is an improvement in a method for the determination of an analyte that is a member of a specific binding pair in a sample suspected of containing such analyte. The method comprises the steps of (a) providing in an assay medium the sample and a binding partner for the analyte and (b) detecting the binding of the binding partner to the analyte. The improvement comprises including in the assay medium a compound of the invention in an amount sufficient to enhance the accuracy of the determination. The invention has particular application to a method for releasing mycophenolic acid from a complex thereof.

Abstract: Proliferative skin diseases such as psoriasis are treated by topically administering cAMP analogs of the following formula to the afflicted skin site at a dose that preferentially and significantly activates type II protein kinase: ##STR1## where: R.sub.6 is:(i) --NR.sub.1 R.sub.2 where R.sub.1 and R.sub.2 are independently selected from the group consisting of hydrogen, alkyl of 1 to 5 carbon atoms, and cyclopentyl or are joined together with the nitrogen atom to form a hetrocyclic group having 4 to 5, inclusive, annular carbon atoms with the proviso that the total number of carbon atoms in the --NR.sub.1 R.sub.2 group is 4 or 5, or(ii) --XR where X is a chalcogen atom of atomic number 8 or 16 and R is alkyl of 4 or 5 carbon atoms,R.sub.8 is:(i) --X--(CH.sub.2).sub.n R.sub.3 where X is defined previously, n is an integer in the range of 1 to 3, inclusive, R.sub.