Patents by Inventor Whei-Kuo Wu

Whei-Kuo Wu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Sample processing to release nucleic acids for direct detection
    Patent number: 6448047
    Abstract: The present invention describes compositions and methods for releasing nucleic acids from cells in a form that is suitable for labeling/capture, amplification, or detection in a single reagent addition step. The compositions include a lipid, membrane fluidizing compound, enzyme for degrading cell structure, metal chelators, or one or more nucleic acid probes or primers complementary to the nucleic acid to be detected. The compositions are non-denaturing and non-inhibitory of enzymes or proteins that are used in nucleic acid release, amplification, labeling or detection. The invention also provides kits for performing the above methods.
    Type: Grant
    Filed: April 30, 2001
    Date of Patent: September 10, 2002
    Assignee: Applied Gene Technologies, Inc.
    Inventors: Nanibhushan Dattagupta, C. Nagaraja Sridhar, Whei-Kuo Wu
  • Method of labeling a nucleic acid amplicon with simultaneous contamination prevention
    Patent number: 6423497
    Abstract: This invention relates to methods of amplifying nucleic acids to minimize contamination by products of earlier amplification reactions. More particularly, it relates to methods of using nucleic acid labels that inhibit further amplification of the amplicon, and compositions that are useful to accomplish this task. In particular, the present invention relates to photoreactive complexes of a binding ligand, a binding enhancer and a label.
    Type: Grant
    Filed: August 9, 2000
    Date of Patent: July 23, 2002
    Assignee: Applied Gene Technologies, Inc.
    Inventors: Nanibhushan Dattagupta, C. Nagaraja Sridhar, Whei-Kuo Wu
  • Stabilization of nucleic acid amplification cocktails
    Publication number: 20020061537
    Abstract: The present invention provides a cocktail of reagents for nucleic acid amplification that are stabilized by inclusion of a reversible inhibitor of undesirable reactions. Such cocktail of reagents eliminates the requirement for separate preparation and quality control of each reagent used in a reaction. Methods to prepare stabilized cocktails and to use stabilized cocktails also are included. The stabilized cocktail compositions also can include reagents to release nucleic acid from cells and to label the nucleic acid, allowing detection of nucleic acid in a sample with a single reagent addition step. The invention also provides kits for performing the above methods.
    Type: Application
    Filed: January 14, 2002
    Publication date: May 23, 2002
    Inventors: Nanibhushan Dattagupta, C. Nagaraja Sridhar, Whei-Kuo Wu
  • Stabilization of nucleic acid amplification cocktails
    Patent number: 6379930
    Abstract: The present invention provides a cocktail of reagents for nucleic acid amplification that are stabilized by inclusion of a reversible inhibitor of undesirable reactions. Such cocktail of reagents eliminates the requirement for separate preparation and quality control of each reagent used in a reaction. Methods to prepare stabilized cocktails and to use stabilized cocktails also are included. The stabilized cocktail compositions also can include reagents to release nucleic acid from cells and to label the nucleic acid, allowing detection of nucleic acid in a sample with a single reagent addition step. The invention also provides kits for performing the above methods.
    Type: Grant
    Filed: August 26, 1999
    Date of Patent: April 30, 2002
    Assignee: Applied Gene Technologies, Inc.
    Inventors: Nanibhushan Dattagupta, C. Nagaraja Sridhar, Whei-Kuo Wu
  • Sample processing to release nucleic acids for direct detection
    Publication number: 20010031473
    Abstract: The present invention describes compositions and methods for releasing nucleic acids from cells in a form that is suitable for labeling/capture, amplification, or detection in a single reagent addition step. The compositions include a lipid, membrane fluidizing compound, enzyme for degrading cell structure, metal chelators, or one or more nucleic acid probes or primers complementary to the nucleic acid to be detected. The compositions are non-denaturing and non-inhibitory of enzymes or proteins that are used in nucleic acid release, amplification, labeling or detection. The invention also provides kits for performing the above methods.
    Type: Application
    Filed: April 30, 2001
    Publication date: October 18, 2001
    Inventors: Nanibhushan Dattagupta, C. Nagaraja Sridhar, Whei-Kuo Wu
  • Sample processing to release nucleic acids for direct detection
    Patent number: 6242188
    Abstract: The present invention describes compositions and methods for releasing nucleic acids from cells in a form that is suitable for labeling/capture, amplification, or detection in a single reagent addition step. The compositions include a lipid, membrane fluidizing compound, enzyme for degrading cell structure, metal chelators, or one or more nucleic acid probes or primers complementary to the nucleic acid to be detected. The compositions are non-denaturing and non-inhibitory of enzymes or proteins that are used in nucleic acid release, amplification, labeling or detection. The invention also provides kits for performing the above methods.
    Type: Grant
    Filed: August 26, 1999
    Date of Patent: June 5, 2001
    Assignee: Applied Gene Technologies, Inc.
    Inventors: Nanibhushan Dattagupta, C. Nagaraja Sridhar, Whei-Kuo Wu
  • Method of labeling a nucleic acid amplicon with simultaneous contamination prevention
    Patent number: 6187566
    Abstract: This invention relates to methods of amplifying nucleic acids to minimize contamination by products of earlier amplification reactions. More particularly, it relates to methods of using nucleic acid labels that inhibit further amplification of the amplicon, and compositions that are useful to accomplish this task. In particular, the present invention relates to photoreactive complexes of a binding ligand, a binding enhancer and a label.
    Type: Grant
    Filed: March 9, 1999
    Date of Patent: February 13, 2001
    Assignee: Applied Gene Technologies, Inc.
    Inventors: Nanibhushan Dattagupta, C. Nagaraja Sridhar, Whei-Kuo Wu