Abstract: The disclosed invention relates to a method for identifying biomarkers for the stratification of hypertensive patients among different hypertension diseases: endocrine forms of hypertension and primary hypertension. The method is a machine-learning based method using one trained classifier on a predefined input dataset to rank several combinations of omics biomarkers (miRNA, steroids, metanephrines, small metabolites) on the basis of the computation of at least one evaluation parameter. A combination of biomarkers is selected to stratify the hypertensive patient among said plurality of hypertension diseases. Also, the disclosed invention relates to a method for stratifying hypertensive patients, such as hypertensive patients with endocrine forms of hypertension (EHT).

Abstract: The invention provides a method of characterising a disease state comprising: (i) collecting metabolic data from a plurality of subjects; (ii) presenting the data as vectors with dimensions corresponding to different biomarkers: and (iii) weighting the importance of either individual dimensions, or the interplay among multiple dimensions when calculating angles of the vectors, such that there is a minimum variation of angle within a disease class and/or a maximum variation of angle compared to a different disease class. The invention also describes a method of identifying a disease state or following progression of a disease state in a subject comprising: (i) collecting metabolic data from the subject; (ii) presenting the data as vectors with dimensions corresponding to different biomarkers: and (iii) comparing two or more angles of vectors with a prototype vector and optionally at least one relevance matrix, to identify the presence of, or progression of, a disease state.

Abstract: The invention relates to methods, assays and kits for detecting or monitoring adrenal tumours by detecting one or more adrenal steroid metabolites. The metabolites are selected from THS (tetrahydro-11-desoxycortisol), 5-PT (5-pregnenetriol), 5-PD (pregnenediol), PD (pregnanediol), PT (pregnanetriol), Et (etiolanolone), TH-DOC (tetrahydro-11-corticosterone), (5?-THA) 5?-tetra-11-hydrocorticosterone, and 5?-THF (5?-tetra hydrocortisol. An (Androsterone) may also be measured. 6?-OH-cortisol may be measured to monitor mitotane therapy.