Abstract: The invention relates to a method for characterizing a cell or tissue culture sample based on multiple cellular signaling pathway activities. The cellular signaling pathway activities are preferably determined based on expression levels of target genes of the respective pathways. The invention further describes products useful for the method having primers for the detection of pathway target genes, and uses of the product.

Abstract: The invention relates to methods based on gene expression levels to predict treatment response to an anti-TNFalpha compound in inflammatory bowel disease patients based on a pre-treatment biopsy, and a method of determining the treatment effectiveness of such treatment based on a biopsy obtained after initiation of the treatment with an anti-TNFalpha compound. The prediction is based on the determination of cellular signaling pathway activity, in particular a pathway selected from TGFbeta, NFkB, MAPK-AP1, STAT3 and WNT, preferably wherein the cellular signaling pathway is selected from TGFbeta and NFkB. When determining the treatment effectiveness, the cellular signaling pathway is AR and/or MAPK-AP1 if the IBD is UC and wherein if the IBD is CD, the cellular signaling pathway is MAPK-AP1 and/or NFkB.

Abstract: The invention relates to a method for determining whether a subject with an infection has a viral infection. The invention further relates to method for determining the cellular immune response to a viral infection or a vaccine. The methods may be performed on a blood sample obtained from a subject, and is based on the finding that specific cellular signaling pathways are active. The invention further relates to components for performing the methods and use of those components in a method of diagnosis.

Abstract: The present invention relates to means and methods that can be used—based on a blood sample of a subject having sepsis, a subject suspected to have sepsis or a subject at risk to develop sepsis, to diagnose the subject with sepsis. The methods can further be used for making a prediction, e.g. whether the subject is likely to develop sepsis, or whether the subject has a high mortality risk as a result of sepsis. The invention further provides for compounds for use in the treatment or prevention of sepsis.

Abstract: A simplified method of obtaining purified nucleic acids uses a single buffer solution to both wash and elute nucleic acids bound to a binding phase. Use of a single buffer solution avoids the time-consuming aspect of using different wash and elution buffer solutions during multiple nucleic acid purification steps. The method for purifying nucleic acids using a single buffer solution includes steps of: exposing a sample comprising nucleic acids to a nucleic acid binding phase, where the nucleic acid binding phase may include magnetic particles, silica particles, or a mixture thereof; and allowing the nucleic acids to bind to the nucleic acid binding phase; washing the nucleic acids bound to the nucleic acid binding phase at least once with the single buffer solution at room temperature; and eluting the nucleic acids from the nucleic acid binding phase with ?50 ?l of the single buffer solution, at a temperature of ?40° Celsius.

Abstract: In order to provide a system allowing an improved and facilitated preparation of a sample for an appropriate analysis, a device for preparing a patient's sample before analysis is provided. The device comprises a housing (10, 30) and an actuator (50). The housing includes a receiving chamber (32). The receiving chamber is configured for receiving a liquid. The actuator is movable relative to the housing. The actuator is configured for extracting a predetermined amount of the liquid received in the receiving chamber and for supplying the extracted liquid to a reagent chamber, so that the reagent is suspended in the liquid. The actuator comprises a metering chamber (52) configured for receiving the predetermined amount of the liquid and for supplying the predetermined amount of the liquid to the reagent chamber.

Abstract: In order to provide a system allowing an improved and facilitated preparation of a sample for an appropriate analysis, a device for preparing a patient's sample before analysis is provided. The device comprises a housing (10, 30) and an actuator (50). The housing includes a receiving chamber (32). The receiving chamber is configured for receiving a liquid. The actuator is movable relative to the housing. The actuator is configured for extracting a predetermined amount of the liquid received in the receiving chamber and for supplying the extracted liquid to a reagent chamber, so that the reagent is suspended in the liquid. The actuator comprises a metering chamber (52) configured for receiving the predetermined amount of the liquid and for supplying the predetermined amount of the liquid to the reagent chamber.

Abstract: The present invention relates to the purification of nucleic acids by an efficient and cost-effective method. In particular, the present invention addresses the laborious and time-consuming aspect of using different wash and elution buffer solutions during multiple, purification steps by using a single wash and elution buffer for nucleic acid purification. The use of a single wash and elution buffer solution, particularly in miniaturized environments, 10 represents a simplified way of obtaining purified nucleic acids in high amounts.