Abstract: A holding device for a dried biological fluid spotting membrane includes a plastic top face, a plastic bottom face, and a pocket disposed between the top face and the bottom face. The plastic top face includes a window formed therein. The pocket is configured for receiving a dried biological fluid spotting membrane that is accessible through the window by a user during dried biological fluid spot testing. A method for assembling a holding device includes positioning the membrane within a pocket of a plastic membrane housing, and securing the membrane within the pocket. The holding device may be utilized for forming dried biological fluid spots and extracting samples therefrom for analysis.

Abstract: Solid phase extraction devices for sample preparation are disclosed, comprising a hollow conical tube having one narrower opening and one broader opening, wherein the narrower opening of the tube contains a solid phase extraction material comprising a functionalized monolithic sorbent, and wherein the solid phase extraction device is prepared by a combination of reduced pressure, positive pressure and mechanical compaction. The solid phase extraction devices are adapted for preparation of small sample volumes, and provide excellent recovery of analytes and good flow characteristics. Methods for preparing and using the solid phase extraction devices are disclosed.