Abstract: A method for isolating plasmids from suspended bacterial or yeast cells using a filter matrix, at least one lysing substance being added to the suspension and predamaging or completely lysing the predominant portion of the suspended cells, at least one conformation-altering substance being added to the suspension to alter the conformation of the plasmids to be isolated so as to promote retention of the cell components in or at the filter matrix. The suspension is thereupon moved through the filter matrix and the cells if not yet lysed then being lysed to completion upon contact with the filter matrix and the released plasmids being retained in or at the filter matrix.

Abstract: Application of a functionalized porous membrane to purify nucleic acids, the binding properties of the membrane with respect to nucleic acids being adjustable by controlling the conditions of an ambient medium. The membrane is functionalized by deprotonated groups.

Abstract: A configuration of mini-volume reaction receptacles (1, 16, 41) of which the receptacle housings (2, 17) each enclose an elongated chamber (3, 18, 42) of which the ends are connected to apertures (6, 7, 20, 22) formed in the receptacle housing. The receptacle housings have identical base surfaces and have a small height relative to the base surface, and are stacked on one another while their base surfaces are mutually aligned. At least one aperture of a receptacle housing communicates with at least one aperture of a vertically adjacent receptacle housing, as seen in the direction of stacking. The receptacles (1, 16, 41) are mechanically interlocked in a direction transverse to the direction of stacking and can be plugged one into another. Each receptacle housing defines at least one aperture (6, 7, 22) at its top side that is accessible to a pipette.

Abstract: The invention relates to a method for purifying nucleic acids contained in a raw material using a porous membrane, the raw material possibly being prepurified and then mixed with a binding buffer containing a precipitant at a concentration such that the final concentration of the mixture of raw material and binding buffer it shall exceed the concentration required to precipitate nucleic acids, the raw material containing the nucleic acids being allowed to permeate the membrane in a manner that the nucleic acids shall be selectively retained at the membrane's surface or in its pores, the membrane being washed if called for and optionally the bound nucleic acids being eluted again from the membrane in a further step.

Abstract: A configuration of mini-volume reaction receptacles (1, 16, 41) of which the housings (2, 17) each enclose an elongated chamber (3, 18, 42) of which the ends are connected to apertures (6, 7, 20, 22) of the particular housing, said housings exhibiting the same base surfaces and being of low height compared to the base surface and are stacked one on another while their base surfaces are mutually aligned, at least one aperture of a receptacle communicating with at least one aperture of a consecutive receptacle as seen in the direction of stacking, said configuration being characterized in that the receptacles (1, 16, 41) are mechanically interlocked in the direction transverse to the direction of stacking and can be plugged one into another, and each receptacle comprises at least one aperture (6, 7, 22) accessible to a pipette at its top side.

Abstract: The invention relates to a method for isolating plasmids from suspended bacterial or yeast cells using a filter matrix, at least one lysing substance being added to the suspension and pre-damaging or completely lysing the predominant portion of the suspended cells, at least one conformation-altering substance being added to said suspension to alter the conformation of the plasmids to be isolated in such manner as to promote retention of the cell components in or at the filter matrix, the suspension thereupon being moved through a filter matrix and the cells if not yet lysed then being lysed to completion upon contact with the filter matrix and the released plasmids being retained in or at the said filter matrix.

Abstract: A method for the isolation of nucleic acids from a liquid sample, which contains nucleic acids, using a filtering device, which has pores, through which the sample is allowed to flow in the course of the isolation, the nucleic acids, and contained in the sample, being retained selectively in or at the pores, wherein the sample is mixed with a concentration of the precipitating agent for nucleic acids, which is sufficient to condense the nucleic acids and the sample is then allowed to flow through the filtering device, the pores of the filtering device used having inner wall regions with variable surface structures, which are constructed differently depending on the medium passed through the pores and by which the permeability of the pores can be adjusted between a state, in which the condensed nucleic acid molecules are retained, and a state, in which the dissolved nucleic acid molecules can pass through.

Abstract: A method for the purification and subsequent determination of double-strand DNA in which the DNA is immobilized on a solid phase, wherein during purification the DNA is immobilized on a probe using a residue having an affinity for DNA, whereby the probe itself is bound to a solid phase or is coupled in a later step to a solid phase and the solid phase is the surface of a reaction vessel, a chip or a tip and subsequent further purification for purposes of determination of the DNA is initiated directly using the nucleic acids immobilized on the solid phase.

Abstract: A process for the production of surface-functionalized supports that serve as starting products for the production of microarrays for immobilizing biomolecules, in which process the surface of a support is coated with an initiator and the coated surface is then put in contact with a solution containing at least one first group of polymerizable monomers, whereby the monomers contain binding sites onto which the biomolecules (probe molecules) can bond, and whereby the conditions under which the monomers are put in contact with the activated support are selected in such a way that the monomers, mediated by the initiator, bind to the support and, on that basis, polymerize to form functional polymer chains in such a manner that a fixed structure consisting of adjacent functional polymer chains is formed on the surface of the support.

Abstract: A method for the isolation of nucleic acids from a liquid sample, which contains nucleic acids, using a filtering device, which has pores, through which the sample is allowed to flow in the course of the isolation, the nucleic acids, and contained in the sample, being retained selectively in or at the pores, wherein the sample is mixed with a concentration of the precipitating agent for nucleic acids, which is sufficient to condense the nucleic acids and the sample is then allowed to flow through the filtering device, the pores of the filtering device used having inner wall regions with variable surface structures, which are constructed differently depending on the medium passed through the pores and by means of which the permeability of the pores can be adjusted between a state, in which the condensed nucleic acid molecules are retained, and a state, in which the dissolved nucleic acid molecules can pass through.