Patents by Inventor Wilhelm Winckler
Wilhelm Winckler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 4746731Abstract: A protein PP.sub.18 is isolated having the following characteristics:(a) an electrophoretic mobility in the region of that of .beta..sub.1 -globulins;(b) an isoelectric point between 5.6 and 6.2;(c) a sedimentation coefficient s.sub.20,w of 5.0.+-.0.2 S;(d) a molecular weight determined in an ultracentrifuge of 82,300.+-.5,600;(e) a carbohydrate fraction of 2.3.+-.1.3 g/100 g (mannose 0.15.+-.0.1, xylose 0.5.+-.0.5, galactose 0.5.+-.0.2, glucose 0.2.+-.0.1, N-acetylglucosamine 0.7.+-.0.2, and N-acetylneuraminic acid 0.25.+-.0.2, each g/100 g).Also described is the amino acid composition of the protein and a process for its isolation. Antiserum is prepared and used in an immunoassay to detect the protein.Type: GrantFiled: May 17, 1985Date of Patent: May 24, 1988Assignee: Behringwerke AktiengesellschaftInventors: Hans Bohn, Wilhelm Winckler
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Patent number: 4599318Abstract: The protein PP.sub.19 which has(a) an electrophoretic mobility in the region between that of .alpha..sub.1 and of .beta..sub.1 globulins;(b) an isoelectric point between 4.6 and 5.4;(c) a sedimentation coefficient s.sub.20,w of 3.25.+-.0.25 S;(d) a molecular weight determined in the ultracentrifuge of 36,500.+-.4,000;(e) a carbohydrate fraction of 3.9.+-.1.5 g/100 g (mannose 0.3.+-.0.2, fucose 0.2.+-.0.1, galactose 1.0.+-.0.3, glucose 0.4.+-.0.2, N-acetylglucosamine 1.2.+-.0.3, N-acetylgalactosamine 0.1.+-.0.1, and N-acetylneuraminic acid 0.7.+-.0.3, each g/100 g); and(f) a particular amino acid composition,is described, as is a process for obtaining it.Type: GrantFiled: February 7, 1985Date of Patent: July 8, 1986Assignee: Behringwerke AktiengesellschaftInventors: Hans Bohn, Wilhelm Winckler
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Patent number: 4594328Abstract: The protein PP.sub.21 which has the following characteristics:(a) an electrophoretic mobility in the region of that of .beta..sub.1 -globulins,(b) an isoelectric point of 4.6.+-.0.3;(c) a sedimentation coefficient s.sub.20,w of 3.2.+-.0.2 S;(d) a molecular weight determined in an ultracentrifuge of 52,900.+-.6,200;(e) an extinction coefficient E.sub.1 cm.sup.1% (280 nm) of 10.5.+-.1.0; and(f) a carbohydrate fraction of 19.2.+-.5.2 g/100 g (mannose 1.8.+-.0.4, galactose 4.3.+-.1.0, fucose 1.3+0.3, N-acetylglucosamine 6.5.+-.2.0 and N-acetylneuraminic acid 5.3.+-.1.5, each g/100 g),and a specified amino acid composition, a process for obtaining it and its use are described.Type: GrantFiled: March 21, 1985Date of Patent: June 10, 1986Assignee: Hoechst AktiengesellschaftInventors: Hans Bohn, Wilhelm Winckler
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Patent number: 4592863Abstract: The protein PP.sub.20 is described, this having the following characteristics:(a) an electrophoretic mobility which is somewhat larger than that of albumin,(b) an isoelectric point of 4.65.+-.0.1,(c) a sedimentation coefficient s.sub.20,w of 4.1.+-.0.1 S,(d) a molecular weight determined by electrophoresis in sodium dodecyl sulfate (SDS)-containing polyacrylamide gel of 50,000.+-.10,000, the molecules of PP.sub.20 being composed of apparently identical subunits which have a molecular weight of 27,000.+-.3,000 and are held together non-covalently,(e) an extinction coefficient E.sub.1cm.sup.1% (280 nm) of 9.5.+-.0.6,(f) a carbohydrate content of 3.0.+-.1.3%, including mannose 0.14.+-.0.05%, fucose 0.13.+-.0.05%, galactose 0.61.+-.0.2%, glucose 0.39.+-.0.2%, N-acetylglucosamine 0.95.+-.0.3%, N-acetylgalactosamine 0.18.+-.0.1%, and N-acetylneuraminic acid 0.6.+-.0.4%.(g) a specified aminoacid composition, and a process for obtaining it is described.Type: GrantFiled: October 19, 1984Date of Patent: June 3, 1986Assignee: Behringwerke AktiengesellschaftInventors: Hans Bohn, Wilhelm Winckler
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Patent number: 4468345Abstract: The protein PP.sub.17, which has (a) an electrophoretic mobility in the range between .beta..sub.1 - and .alpha..sub.2 -globulins, (b) an isoelectric point of 5.25.+-.0.20, (c) a sedimentation coefficient S.sub.20.sup.o, w of 2.7.+-.0.1 S, (d) a molecular weight, determined in polyacrylamide gel containing sodium dodecyl sulfate (SDS), of 38,000.+-.2,000, (e) an extinction coefficient E.sub.1cm.sup.1% (280 nm) of 8.5.+-.0.4 and (f) a carbohydrate content of 2.1.+-.0.9% containing 0.3.+-.0.2% of mannose, 0.4.+-.0.2% of galactose, 0.2.+-.0.1% of xylose, 1.0.+-.0.3% of N-acetyl-glucosamine and 0.2.+-.0.1% of N-acetyl-neuramin acid, is described.A process for concentrating and isolating it and its use are also described.Type: GrantFiled: July 29, 1983Date of Patent: August 28, 1984Assignee: Behringwerke AktiengesellschaftInventors: Hans Bohn, Wilhelm Winckler
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Patent number: 4269825Abstract: A new glycoprotein is characterized by a protein consisting essentially of 75.+-.6% of .alpha.-amino-acids, a carbohydrate portion of 24.6.+-.5.2%, which contains 8.9.+-.2% of hexoses, 7.1.+-.1.5 of N-acetylated hexosamine, 0.2.+-.0.2% of fucose, 8.4.+-.1.5% of, N-acetylated neuraminic acid; a sedimentation coefficient S.sub.20w of 2.5.+-.0.3 S; a molecular weight of 35,000.+-.5,000, determined in the ultracentrifuge, and a molecular weight of 65,000.+-.10,000, determined in polyacrylamide gel containing sodium dodecyl sulfate; an iso-electric point at pH 3.4.+-.0.4; an extinction coefficient E.sub.1.sup.1%.sub.cm (280 nm) of 1.9.+-.0.3; an electrophoretic mobility in the range between that of .alpha..sub.1 and .alpha..sub.2 -globulins; and a specific immunologic reaction with an antibody specifically directed against the glycoprotein.Type: GrantFiled: December 19, 1979Date of Patent: May 26, 1981Assignee: Behringwerke AGInventors: Hans Bohn, Wilhelm Winckler
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Patent number: 4254021Abstract: The invention relates to a tissue specific protein, i.e. a substance which is a constituent of parenchymatous tissues, and to a process for preparing the same. The tissue protein is characterized by a protein proportion of 94.+-.3%, essentially consisting of .alpha.-amino acids,an amino acid analysis of______________________________________ Variation Mol % coefficient (%) ______________________________________ lysine 5,64 12.7 histidine 1.05 15.0 arginine 3.83 9.2 aspartic acid 9.84 6.8 threonine 4.33 15.7 serine 4.78 8.2 glutamic acid 11.29 3.5 proline 6.41 9.2 glycine 8.65 9.8 alanine 7.51 6.4 cystine/2 2.11 12.6 valine 6.68 7.9 methionine 0.98 14.5 isoleucine 3.32 7.4 leucine 14.43 4.2 tyrosine 5.10 14.9 phenylalanine 3.08 13.5 and tryptophan 1.14 11.4 ______________________________________a carbohydrate proportion of 5.4.+-.2.2% consisting of 3.0.+-.1% of hexoses, 1.2.+-.0.5% of hexosamine, 0.2.+-.0.2% of fucose, and 1.0.+-.0.5% of neuraminic acid,a sedimentation coefficient S.sub.20,w.sup.c=0.1 of 3.5 S.Type: GrantFiled: August 21, 1979Date of Patent: March 3, 1981Assignee: Behringwerke AktiengesellschaftInventors: Hans Bohn, Wilhelm Winckler
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Patent number: 4217339Abstract: What is disclosed is a glycoprotein having proteolytic, fibrinolytic, and thrombolytic properties and which can be used to prepare anti-sera useful as diagnostic agents, said glycoprotein being found in human blood serum and extracts of human placentas and being capable of isolation therefrom and which has the following properties:a protein proportion essentially consisting of 89.+-.4% of .alpha.-amino-acids,a carbohydrate proportion of 11.1.+-.2.2%, among it 5.3.+-.1.1% of hexoses, 2.8.+-.0.5 N of N-acetylated hexosamine, 2.9.+-.0.6% of N-acetylated neuraminic acid;a sedimentation coefficient S.sub.20x of 3.2.+-.0.3 S;a molecular weight of 32 000.+-.6 000;an iso-electric point at pH 4.3.+-.0.3;an extinction coefficient E.sub.1 cm.sup.1% (280 nm) of 13.8.+-.1.0;an electrophoretic mobility in the range between that of albumin and .alpha..sub.1 -globulins;a specific immunologic reaction with an antibody specifically directed against the protein, anda proteolytic activity, as well as a process for isolating it.Type: GrantFiled: May 4, 1978Date of Patent: August 12, 1980Assignee: Behringwerke AktiengesellschaftInventors: Hans Bohn, Wilhelm Winckler
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Patent number: 4018885Abstract: A derivative of the steroid-binding .beta.-globulin which can be obtained therefrom by treatment with neuraminidase.Type: GrantFiled: October 24, 1974Date of Patent: April 19, 1977Assignee: Behringwerke AktiengesellschaftInventors: Hans Bohn, Wilhelm Winckler