Patents by Inventor Willem P.C. Stemmer

Willem P.C. Stemmer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Modification of virus tropism and host range by viral genome shuffling
    Publication number: 20030124708
    Abstract: The invention relates to a method and compositions for modifying a phenotype of a virus, such as viral tropism and host range, by iterative sequence recombination of variant viruses and selection of improved variants.
    Type: Application
    Filed: December 4, 2002
    Publication date: July 3, 2003
    Applicant: Maxygen, Inc.
    Inventors: Willem P.C. Stemmer, Phillip Patten, Nay Wei Soong
  • Methods and compositions for polypeptide engineering
    Patent number: 6586182
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: October 20, 2000
    Date of Patent: July 1, 2003
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • Methods and compositions for polypeptide engineering
    Patent number: 6579678
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: October 20, 2000
    Date of Patent: June 17, 2003
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • Methods for producing recombined antibodies
    Patent number: 6576467
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: November 21, 2000
    Date of Patent: June 10, 2003
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Polynucleotides encoding flavivirus and alphavirus multivalent antigenic polypeptides
    Patent number: 6576757
    Abstract: This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: June 10, 2003
    Assignee: Maxygen, Inc.
    Inventors: Juha Punnonen, Steven H. Bass, Robert Gerald Whalen, Russell Howard, Willem P. C. Stemmer
  • Nucleic acid libraries
    Patent number: 6573098
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: January 29, 1999
    Date of Patent: June 3, 2003
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Flavivirus and alphavirus recombinant antigen libraries
    Patent number: 6569435
    Abstract: This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: May 27, 2003
    Assignee: Maxygen, Inc.
    Inventors: Juha Punnonen, Steven H. Bass, Robert Gerald Whalen, Russell Howard, Willem P. C. Stemmer
  • Methods and compositions for cellular and metabolic engineering
    Publication number: 20030082611
    Abstract: The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.
    Type: Application
    Filed: September 17, 2002
    Publication date: May 1, 2003
    Applicant: Affymax Technologies N.V.
    Inventors: Jeremy Minshull, Willem P.C. Stemmer
  • Combinatorial libraries of monomer domains
    Publication number: 20030082630
    Abstract: Methods for identifying discrete monomer domains and immuno-domains with a desired property are provided. Methods for generating multimers from two or more selected discrete monomer domains are also provided, along with methods for identifying multimers possessing a desired property. Presentation systems are also provided which present the discrete monomer and/or immuno-domains, selected monomer and/or immuno-domains, multimers and/or selected multimers to allow their selection. Compositions, libraries and cells that express one or more library member, along with kits and integrated systems, are also included in the present invention.
    Type: Application
    Filed: April 26, 2002
    Publication date: May 1, 2003
    Applicant: Maxygen, Inc.
    Inventors: Joost A. Kolkman, Willem P.C. Stemmer
  • Integrated systems and methods for diversity generation and screening
    Publication number: 20030064393
    Abstract: Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.
    Type: Application
    Filed: May 23, 2002
    Publication date: April 3, 2003
    Applicant: Maxygen, Inc.
    Inventors: Steven H. Bass, S. Christopher Davis, Phillip A. Patten, Matthew Tobin, Jeremy Minshull, Mark Welch, Claes Gustafsson, Brian Carr, Stephane Jenne, Sun Ai Raillard, Andreas Crameri, Willem P.C. Stemmer, Robin Emig, Pascal Longchamp, Stanley Goldman, Lorraine J. Giver, Joseph A. Affholter
  • Antigen library immunization
    Patent number: 6541011
    Abstract: This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.
    Type: Grant
    Filed: February 10, 1999
    Date of Patent: April 1, 2003
    Assignee: Maxygen, Inc.
    Inventors: Juha Punnonen, Steven H. Bass, Robert Gerald Whalen, Russell Howard, Willem P. C. Stemmer
  • Integrated systems and methods for diversity generation and screening
    Publication number: 20030054384
    Abstract: Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.
    Type: Application
    Filed: May 23, 2002
    Publication date: March 20, 2003
    Applicant: Maxygen, Inc.
    Inventors: Steven H. Bass, S. Christopher Davis, Phillip A. Patten, Matthew Tobin, Jeremy Minshull, Mark Welch, Claus Gustafsson, Brian Carr, Stephane Jenne, Sun Ai Raillard, Andreas Crameri, Willem P.C. Stemmer, Robin Emig, Pascal Longschamp, Stanley Goldman, Lorraine J. Giver, Joseph A. Affholter
  • Integrated systems and methods for diversity generation and screening
    Publication number: 20030054383
    Abstract: Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.
    Type: Application
    Filed: May 23, 2002
    Publication date: March 20, 2003
    Applicant: Maxygen, Inc.
    Inventors: Steven H. Bass, S. Christopher Davis, Phillip A. Patten, Matthew Tobin, Jeremy Minshull, Mark Welch, Claes Gustafsson, Brian Carr, Stephane Jenne, Sun Ai Raillard, Andreas Crameri, Willem P.C. Stemmer, Robin Emig, Pascal Longschamp, Stanley Goldman, Lorraine J. Giver, Joseph A. Affholter
  • Oligonucleotide mediated nucleic acid recombination
    Publication number: 20030054390
    Abstract: Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
    Type: Application
    Filed: July 15, 2002
    Publication date: March 20, 2003
    Applicant: Maxygen, Inc.
    Inventors: Andreas Crameri, Willem P.C. Stemmer, Jeremy Minshull, Steven H. Bass, Mark Welch, Jon E. Ness, Claes Gustafsson, Phillip A. Patten
  • Subtilisin variants
    Publication number: 20030049827
    Abstract: New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.
    Type: Application
    Filed: April 2, 2001
    Publication date: March 13, 2003
    Inventors: Jon E. Ness, Mark Welch, Lorraine J. Giver, Joel R. Cherry, Torben V. Borchert, Willem P.C. Stemmer, Jeremy Minshull
  • Oligonucloetide mediated nucleic acid recombination
    Patent number: 6521453
    Abstract: Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
    Type: Grant
    Filed: October 23, 2000
    Date of Patent: February 18, 2003
    Assignee: Maxygen, Inc.
    Inventors: Andreas Crameri, Willem P. C. Stemmer, Jeremy Minshull, Steven H. Bass, Mark Welch, Jon E. Ness, Claes Gustafsson, Phillip A. Patten
  • Methods of populating data structures for use in evolutionary simulations
    Publication number: 20030032010
    Abstract: In particular, this invention provides novel methods of populating data structures for use in evolutionary modeling. In particular, this invention provides methods of populating a data structure with a plurality of character strings. The methods involve encoding two or more a biological molecules into character strings to provide a collection of two or more different initial character strings; selecting at least two substrings from the pool of character strings; concatenating the substrings to form one or more product strings about the same length as one or more of the initial character strings; adding the product strings to a collection of strings; and optionally repeating this process using one or more of the product strings as an initial string in the collection of initial character strings.
    Type: Application
    Filed: February 1, 2000
    Publication date: February 13, 2003
    Inventors: Sergey A. Selifonov, Willem P.C. Stemmer
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6518065
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: October 25, 2000
    Date of Patent: February 11, 2003
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Methods and compositions for polypeptide engineering
    Publication number: 20030027156
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Application
    Filed: September 12, 2001
    Publication date: February 6, 2003
    Applicant: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6506602
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: October 25, 2000
    Date of Patent: January 14, 2003
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer