Abstract: The invention provided herein relates to methods for protein synthesis, characterisation and purification on a digital microfluidic device.

Abstract: The invention relates to improved methods of enzymatic solid-supported nucleic acid synthesis that make use of terminal deoxynucleotidyl transferase (TdT) enzymes or modified terminal deoxynucleotidyl transferase (TdT) enzymes on polyacrylamide type supports. The invention further relates to the use of kits comprising said enzymes in a method of solid-supported nucleic acid synthesis.

Abstract: The disclosed method and system relate to generating recommendations for a user. In some embodiments, the method includes capturing organization data with respect to organizations and user data with respect to the user; determining news feed data and social feed data from online platforms, based on the organization data and the user data; analysing the news feed data and the social feed data to determine stand of the each organization and stand of the user on one or more socio-ethical causes; determining match data associated with the user for a predefined time period, based on the user data, the organization data, and the analysis of the news feed data and the social feed data; and controlling user equipment associated with the user during an online browsing session involving at least one of a product and a service offered by at least one of the organizations.

Abstract: A method and system for producing a dynamic digital twin includes a plurality of energy-harvesting sensors that are interrogated by a reader device to acquire real time data of a parameter. The real time data is transmitted to a sensor hub in the platform of internet of things (IoT), and is processed by a fusion processor which can associate the real time sensor data with geometric data defining a physical space or object to provide a digital twin.

Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Abstract: An animal trap includes: a bait, a support, and an elastic band, wherein in a set state the support supports the elastic band in an expanded state, and wherein removal, consumption or destruction of the bait by an animal: causes the elastic band to contract around the animal to kill the animal; or causes the elastic band to drive a kill mechanism to kill the animal. Animal traps may be formed substantially or entirely of biodegradable materials. Animal traps may be adapted for aerial distribution. A trap dispenser may dispense traps as they are triggered.

Abstract: An animal trap includes: a bait, a support, and an elastic band, wherein in a set state the support supports the elastic band in an expanded state, and wherein removal, consumption or destruction of the bait by an animal: causes the elastic band to contract around the animal to kill the animal; or causes the elastic band to drive a kill mechanism to kill the animal. Animal traps may be formed substantially or entirely of biodegradable materials. Animal traps may be adapted for aerial distribution. A trap dispenser may dispense traps as they are triggered.

Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Abstract: A system for authenticating a sender of an Electronic message transmitted over a telephony network assigns a public key and a private key to each of a plurality of mobile telephony numbers and thereby stores a plurality of public key over a blockchain. The public key and the private key are assigned upon registration of a mobile telephony number with a telephony service provider The Electronic message is sent to a recipient over the telephony network by creating a header, in the message, storing content that needs to be sent to a recipient. The system further analyzes the header to determine whether the mobile telephony number is present on the blockchain. If so the system further retrieves a public key corresponding to the mobile telephony number from the blockchain. The sender of the mobile telephony number is authenticated upon verifying the signature by using the public key from the blockchain.

Abstract: Disclosed is a system for authenticating a sender of an E-mail to be sent over a network. The system assigns a public key and a private key to each of a plurality of E-mail addresses and thereby stores a plurality of public key over a blockchain. The system further sends an E-mail to a recipient over a network by creating a header, in the E-mail, storing content that needs to be sent to a recipient. The system further analyzes the header to determine whether the E-mail address is present on the blockchain. If the E-mail address is present, the system further retrieves a public key, of the plurality of public keys, corresponding to the E-mail address from the blockchain. The system further authenticates the sender of the E-mail upon verifying the signature, used to sign the content, by using the public key retrieved for the E-mail address from the blockchain.

Abstract: Disclosed is a system for authenticating a caller. The system assigns a public key and a private key to each Caller Identifications (CLID), of a plurality of CLIDs pertaining to a plurality of mobile telephony numbers. The system stores a plurality of CLIDs and a plurality of public keys, associated to the plurality of CLIDs, over a blockchain formed by the plurality of telephony service providers. The system sends a Multi Data Message Format (MDMF) message to a telephony service provider of a callee. The system analyzes the MDMF message to determine whether the CLID is present over the blockchain. The system enables the telephony service provider of the callee to retrieve a public key, of the plurality of public keys, corresponding to the CLID from the blockchain, when the CLID is present on the blockchain. The system authenticates the caller upon verifying the signature by using the public key.

Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.

Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Abstract: Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.

Abstract: This invention relates to the preparation of nucleic acid samples for analysis. The invention may be particularly useful for single stranded samples. Embodiments of the invention involve the attachment of double stranded or hairpin oligonucleotides using template independent polymerase enzymes in the preparation of nucleic acid sequencing libraries.

Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Abstract: This invention relates to the preparation of nucleic acid samples for analysis. The invention may be particularly useful for single stranded samples. Embodiments of the invention involve the attachment of double stranded or hairpin oligonucleotides using template independent polymerase enzymes in the preparation of nucleic acid sequencing libraries.

Abstract: This invention relates to improved methods and kits for identification of 5-formylcytosine (5fC) to be distinguished from cytosine (C) in a sample nucleotide sequence. Methods comprise reducing a first portion of polynucleotides which comprise the sample nucleotide sequence; treating the reduced first portion and a second portion of polynucleotides with bisulfite; sequencing the polynucleotides in the first and second portions of the population to produce first and second nucleotide sequences respectively and; identifying the residues in the first and second nucleotide sequences which correspond to a cytosine residue in the sample nucleotide sequence. These methods may be useful, for example in the analysis of genomic DNA and/or of RNA.