Patents by Inventor William A. Cuevas

William A. Cuevas has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Alpha-amylase combinatorial variants
    Patent number: 11920170
    Abstract: Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: March 5, 2024
    Assignee: DANISCO US INC.
    Inventors: William Cuevas, Vivek Sharma, David E. Wildes, Sang-Kyu Lee, Dina Finan
  • ALPHA-AMYLASE COMBINATORIAL VARIANTS
    Publication number: 20190144842
    Abstract: Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
    Type: Application
    Filed: December 9, 2016
    Publication date: May 16, 2019
    Inventors: William Cuevas, Vivek Sharma, David E. Wildes, Sang-Kyu Lee, Dina Finan
  • Alpha-Amylase Combinatorial Variants
    Publication number: 20180163191
    Abstract: Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
    Type: Application
    Filed: February 2, 2017
    Publication date: June 14, 2018
    Applicant: Danisco US Inc.
    Inventors: William Cuevas, Vivek Sharma, David E. Wildes, Sang-kyu Lee, Dina Finan
  • VARIANT ALPHA-AMYLASES FROM BACILLUS SUBTILIS AND METHODS OF USE, THEREOF
    Publication number: 20160010129
    Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
    Type: Application
    Filed: June 17, 2015
    Publication date: January 14, 2016
    Inventors: William A. Cuevas, Sang-Kyu Lee, Sandra W. Ramer, Andrew Shaw, Amr R. Toppozada, David A. Estell, Louise Wallace, Regina Chin, Carol A. Requadt, Scott D. Power, Michael J. Pepsin
  • Variant alpha-amylases from and methods of use, thereof
    Patent number: 9090887
    Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
    Type: Grant
    Filed: June 5, 2009
    Date of Patent: July 28, 2015
    Assignee: DANISCO US INC.
    Inventors: William A Cuevas, Sang-Kyu Lee, Sandra W Ramer, Andrew Shaw, Amr R Toppozada, David E Estell, Louise Wallace, Regina Chin, Carol A Requadt, Scott D Power, Michael J Pepsin
  • Production of glucose from starch using alpha-amylases from
    Patent number: 9040278
    Abstract: An ?-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
    Type: Grant
    Filed: June 4, 2009
    Date of Patent: May 26, 2015
    Assignee: DANISCO US INC.
    Inventors: Luis G. Cascao-Pereira, Regina Chin, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Michael J. Pepsin, Scott D. Power, Sandra W. Ramer, Carol A. Requadt, Andrew Shaw, Amr R. Toppozada, Louise Wallace
  • Variant alpha-amylases from and methods of uses, thereof
    Patent number: 8975056
    Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: March 10, 2015
    Assignee: Danisco US Inc.
    Inventors: Luis G. Cascao-Pereira, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Scott D. Power, Sandra W. Ramer, Amr Toppozada, Louise Wallace
  • Production of isoprenoids under neutral pH conditions
    Patent number: 8951764
    Abstract: Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
    Type: Grant
    Filed: August 3, 2012
    Date of Patent: February 10, 2015
    Assignee: Danisco US Inc.
    Inventors: Martien H. Bergsma, Anthony R. Calabria, Gopal K. Chotani, William A. Cuevas, Gang Duan, Sung Ho Lee, Ying Qian, Vivek Sharma, Jayarama K. Shetty, Bruce A. Strohm, Paula Johanna Maria Teunissen, Hongxian Xu
  • PRODUCTION OF ISOPRENE UNDER NEUTRAL pH CONDITIONS
    Publication number: 20140273145
    Abstract: Embodiments of the present disclosure relate to a process for producing isoprene from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
    Type: Application
    Filed: March 18, 2014
    Publication date: September 18, 2014
    Applicants: THE GOODYEAR TIRE & RUBBER COMPANY, Danisco US Inc.
    Inventors: Martien H. BERGSMA, Anthony R. CALABRIA, Gopal K. CHOTANI, William A. CUEVAS, Gang DUAN, Sung Ho LEE, Ying QIAN, Vivek SHARMA, Jayarama K. SHETTY, Bruce A. STROHM, Paula Johanna Maria TEUNISSEN, Hongxian XU
  • PRODUCTION OF ISOPRENOIDS UNDER NEUTRAL pH CONDITIONS
    Publication number: 20130203140
    Abstract: Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
    Type: Application
    Filed: August 3, 2012
    Publication date: August 8, 2013
    Applicant: Danisco US Inc.
    Inventors: Martien H. BERGSMA, Anthony R. Calabria, Gopal K. Chotani, William A. Cuevas, Gang Duan, Sung Ho Lee, Ying Qian, Vivek Sharma, Jayarama K. Shetty, Bruce A. Strohm, Paula Johanna Maria Teunissen, Hongxian Xu
  • Variant alpha-amylases from and methods of uses, thereof
    Patent number: 8323945
    Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
    Type: Grant
    Filed: June 5, 2009
    Date of Patent: December 4, 2012
    Assignee: Danisco US Inc.
    Inventors: Luis G. Cascao-Pereira, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Scott D. Power, Sandra W. Ramer, Amr Toppozada, Louise Wallace
  • USE OF REPEAT SEQUENCE PROTEIN POLYMERS IN PERSONAL CARE COMPOSITIONS
    Publication number: 20120276021
    Abstract: A personal care composition is provided and includes an effective amount of a repeat sequence protein polymer. The personal care composition may be a hair care composition, a skin care composition, a nail care composition, a cosmetic composition, or an over-the-counter pharmaceutical composition.
    Type: Application
    Filed: September 22, 2011
    Publication date: November 1, 2012
    Applicant: DANISCO US INC.
    Inventors: Manoj Kumar, William A. Cuevas
  • GEOBACILLUS STEAROTHERMOPHILUS ALPHA AMYLASE (AMYS) VARIANTS WITH IMPROVED PROPERTIES
    Publication number: 20120156733
    Abstract: Described are variants of a parent ?-amylase that exhibits an alteration in at least one of the following properties relative to said parent ?-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.
    Type: Application
    Filed: November 8, 2011
    Publication date: June 21, 2012
    Applicant: DANISCO US INC.
    Inventors: William A. Cuevas, Sang-Kyu Lee, Sandra W. Ramer, Andrew Shaw, Amr R. Toppozada, David A. Estell, Sura Hadi
  • PRODUCTION OF ISOPRENE UNDER NEUTRAL pH CONDITIONS
    Publication number: 20120045812
    Abstract: Embodiments of the present disclosure relate to a process for producing isoprene from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
    Type: Application
    Filed: August 5, 2011
    Publication date: February 23, 2012
    Applicant: Danisco US Inc.
    Inventors: Martien H. Bergsma, Anthony R. Calabria, Gopal K. Chotani, William A. Cuevas, Gang Duan, Sung H. Lee, Ying Qian, Vivek Sharma, Jayarama K. Shetty, Bruce A. Strohm, Paula Johanna Maria Teunissen, Hongxian Xu
  • NEUTRAL pH SACCHARIFICATION AND FERMENTATION
    Publication number: 20120034659
    Abstract: Embodiments of the present disclosure relate to a process for producing downstream products, such as fermentable sugars and end products, from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
    Type: Application
    Filed: August 5, 2011
    Publication date: February 9, 2012
    Applicant: Danisco US Inc.
    Inventors: Martien H. Bergsma, Gopal K. Chotani, William A. Cuevas, Gang Duan, Sung Ho Lee, Ying Qian, Vivek Sharma, Jayarama K. Shetty, Bruce A. Strohm, Paula Johanna Maria Teunissen, Hongxian Xu
  • ?-amylase (AmyS) variants with improved properties
    Patent number: 8084240
    Abstract: Described are variants of a parent ?-amylase that exhibits an alteration in at least one of the following properties relative to said parent ?-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.
    Type: Grant
    Filed: June 2, 2009
    Date of Patent: December 27, 2011
    Assignee: Danisco US Inc.
    Inventors: William A Cuevas, Sang-Kyu Lee, Sandra W Ramer, Andrew Shaw, Amr R Toppozada, David E Estell, Sura H Hadi
  • Use of repeat sequence protein polymers in personal care compositions
    Patent number: 8048859
    Abstract: A personal care composition is provided and includes an effective amount of a repeat sequence protein polymer. The personal care composition may be a hair care composition, a skin care composition, a nail care composition, a cosmetic composition, or an over-the-counter pharmaceutical composition.
    Type: Grant
    Filed: September 25, 2007
    Date of Patent: November 1, 2011
    Assignee: Danisco US Inc.
    Inventors: Manoj Kumar, William A. Cuevas
  • Repeat sequence protein polymer active agent congjugates, methods and uses
    Patent number: 7691806
    Abstract: Biomolecular conjugates are provided which comprise the conjugation product of a repeat sequence protein polymer and at least one active agent. Additional aspects provide methods for their manufacture and various industrial and consumer applications.
    Type: Grant
    Filed: February 10, 2006
    Date of Patent: April 6, 2010
    Assignee: Genencor International, Inc.
    Inventors: Katherine D. Collier, William A. Cuevas, Manoj Kumar
  • Variant Alpha-Amylases from Bacillus Subtilis and Methods of Uses, Thereof
    Publication number: 20100015686
    Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
    Type: Application
    Filed: June 5, 2009
    Publication date: January 21, 2010
    Applicant: Danisco US Inc., Genencor Division
    Inventors: Luis G. Cascao-Pereira, William A. Cuevas, David A. Estell, Sang-Kyu Lee, Scott D. Power, Sandra W. Ramer, Amr Toppozada, Louise Wallace
  • Variant Alpha-Amylases from Bacillus Subtilis and Methods of Use, Thereof
    Publication number: 20100003366
    Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
    Type: Application
    Filed: June 5, 2009
    Publication date: January 7, 2010
    Applicant: Danisco US Inc., Genencor Division
    Inventors: William A. Cuevas, Sang-Kyu Lee, Sandra W. Ramer, Andrew Shaw, Amr R. Toppozada, David E. Estell, Louise Wallace, Regina Chin, Carol A. Requadt, Scott D. Power, Michael J. Pepsin