Patents by Inventor William A. Drohan

William A. Drohan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20110039302
    Abstract: Provided herein are methods and compositions for producing Factor VIII proteins. Such methods include introducing into a cell a nucleic acid molecule encoding a Factor VIII protein operably linked to a promoter, wherein the promoter is characterized by the ability to produce commercially viable Factor VIII protein; and incubating the cell under conditions for producing commercially viable Factor VIII protein. Also provided are nucleic acid molecules which encode a Factor VIII protein operably linked to a Chinese hamster elongation factor 1-? (CHEF1) promoter, which may be used in the methods provided herein.
    Type: Application
    Filed: April 12, 2010
    Publication date: February 17, 2011
    Inventors: RANDAL J. KAUFMAN, STEVEN W. PIPE, MICHAEL GRIFFITH, WILLIAM DROHAN
  • Publication number: 20090214382
    Abstract: Methods are disclosed for sterilizing biological materials to reduce the level of one or more biological contaminants or pathogens therein, such as viruses, bacterial (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, single or multicellular parasites, and/or prions or similar agents responsible. These methods involve the use of alpha-keto acids in methods of sterilizing biological materials with irradiation.
    Type: Application
    Filed: May 5, 2005
    Publication date: August 27, 2009
    Inventors: Wilson Burgess, David Mann, William Drohan, Shirley Miekka
  • Publication number: 20080080998
    Abstract: Methods are disclosed for sterilizing tissue to reduce the level of one or more active biological contaminants or pathogens therein, such as viruses, bacteria, (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, prions or similar agents responsible, alone or in combination, for TSEs and/or single or multicellular parasites. The methods involve sterilizing one or more tissues with irradiation.
    Type: Application
    Filed: July 16, 2007
    Publication date: April 3, 2008
    Inventors: Wilson Burgess, William Drohan, Martin MacPhee, David Mann
  • Publication number: 20080070251
    Abstract: Provided herein are methods and compositions for producing Factor VIII proteins. Such methods include introducing into a cell a nucleic acid molecule encoding a Factor VIII protein operably linked to a promoter, wherein the promoter is characterized by the ability to produce commercially viable Factor VIII protein; and incubating the cell under conditions for producing commercially viable Factor VIII protein. Also provided are nucleic acid molecules which encode a Factor VIII protein operably linked to a Chinese hamster elongation factor 1-? (CHEF1) promoter, which may be used in the methods provided herein.
    Type: Application
    Filed: June 29, 2007
    Publication date: March 20, 2008
    Inventors: Randal Kaufman, Steven Pipe, Michael Griffith, William Drohan
  • Publication number: 20080045453
    Abstract: The invention relates to commercially viable methods for producing biologically active vitamin K dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15 mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent ?-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
    Type: Application
    Filed: December 21, 2006
    Publication date: February 21, 2008
    Inventors: William Drohan, Michael Griffith, John Taylor, Darrell Stafford
  • Publication number: 20060287228
    Abstract: Recombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention. Transgenic animals of the present invention are produced by introducing into developing embryos DNA that encodes Factor IX, such that the foreign DNA is stably incorporated in the DNA of germ line cells of the mature animal. Particularly efficient expression was accomplished using a chimeric construct comprising a mammary gland specific promoter, Factor IX cDNA that lacked the complete or any portion of the 5?-untranslated and 3?-untranslated region, which is substituted with a 5-? and 3?-end of the mouse whey acidic protein gene. In vitro cell cultures of cells explanted from the transgenic mammal of the invention and methods of producing Factor IX from such said culture and methods of treating hemophilia B are also described.
    Type: Application
    Filed: April 29, 2005
    Publication date: December 21, 2006
    Inventors: William Velander, William Drohan, Henryk Lubon, John Johnson, Mary Johnson
  • Publication number: 20060182652
    Abstract: Methods are disclosed for sterilizing biological materials to reduce the level of active biological contaminants or pathogens such as viruses, bacteria, nanobacteria, yeasts, molds, mycoplasmas, ureaplasmas, prions and parasites. These methods involve the use of dipeptide stabilizers in methods of sterilizing biological materials with irradiation.
    Type: Application
    Filed: April 7, 2006
    Publication date: August 17, 2006
    Inventors: Wilson Burgess, William Drohan, Martin MacPhee, David Mann, Ewa Maddox
  • Publication number: 20060174357
    Abstract: A transgenic, non-human mammalian animal is capable of expressing a heterologous gene for human or other recombinant physiologically functional fibrinogen holoprotein or individual subunit chain polypeptides thereof or a modified or fusion fibrinogen in mammary glands of the animals and secreting the expressed product into a body fluid. Methodology employing such a mammal yields recombinant physiologically functional fibrinogens, subunit chain polypeptides thereof, and modified or fusion fibrinogens.
    Type: Application
    Filed: January 24, 2005
    Publication date: August 3, 2006
    Inventors: William Velander, Susan Lord, William Drohan, Henryk Lubon, John Johnson, Christopher Russell
  • Publication number: 20060115376
    Abstract: Methods are disclosed for sterilizing preparations of digestive enzymes to reduce the level of one or more active biological contaminants or pathogens therein, such as viruses, bacteria (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, prions or similar agents responsible, alone or in combination, for TSEs and/or single or multicellular parasites. These methods involve sterilizing preparations of digestive enzymes, such as trypsin, ?-galactosidase and iduronate-2-sulfatase, with irradiation.
    Type: Application
    Filed: January 9, 2006
    Publication date: June 1, 2006
    Inventors: David Mann, Wilson Burgess, William Drohan, Yuri Griko, Martin MacPhee
  • Publication number: 20050202003
    Abstract: Methods are disclosed for sterilizing preparation of monoclonal immunoglobulins to reduce the level of active biological contaminants such as viruses, bacteria, yeasts, molds, mycoplasmas, prions and parasites.
    Type: Application
    Filed: April 28, 2005
    Publication date: September 15, 2005
    Inventors: Teri Grieb, Wilson Burgess, William Drohan, Ren-Yo Forng, Martin MacPhee, David Mann, Anna McBain
  • Publication number: 20050106728
    Abstract: Methods are disclosed for sterilizing biological materials to reduce the level of one or more biological contaminants or pathogens therein, such as viruses, bacteria (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, single or multicellular parasites, and/or prions or similar agents responsible, alone or in combination, for TSEs. These methods involve the use of stabilizer mixtures in methods of sterilizing biological materials with irradiation.
    Type: Application
    Filed: October 21, 2004
    Publication date: May 19, 2005
    Inventors: Wilson Burgess, William Drohan, Martin MacPhee, David Mann
  • Publication number: 20050069453
    Abstract: Methods are disclosed for sterilizing preparations of urokinase to reduce the level therein of one or more active biological contaminants or pathogens, such as viruses, bacteria (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia and rickettsias), yeasts, molds, fungi, single or multicellular parasites, and prions or similar agents responsible, alone or in combination, for TSEs. These methods involve sterilizing preparations of urokinase with irradiation.
    Type: Application
    Filed: September 29, 2003
    Publication date: March 31, 2005
    Inventors: Ren-Yo Forng, David Mann, Wilson Burgess, William Drohan, Martin MacPhee, Shirley Miekka
  • Publication number: 20050048637
    Abstract: Methods are disclosed for sterilizing preparations of glycosidases to reduce the level therein of one or more active biological contaminants or pathogens, such as viruses, bacteria (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, single or multicellular parasites, prions or similar agents responsible, alone or in combination, for TSEs. These methods involve sterilizing preparations of glycosidases, such as alpha-glucosidase or alpha-galactosidase, with irradiation.
    Type: Application
    Filed: May 7, 2004
    Publication date: March 3, 2005
    Inventors: William Drohan, Wilson Burgess, David Mann, Martin MacPhee
  • Publication number: 20050047958
    Abstract: Methods are disclosed for sterilizing preparations of digestive enzymes to reduce the level of one or more active biological contaminants or pathogens therein, such as viruses, bacteria (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, prions or similar agents responsible, alone or in combination, for TSEs and/or single or multicellular parasites. These methods involve sterilizing preparations of digestive enzymes, such as trypsin, ?-galactosidase and iduronate-2-sulfatase, with irradiation.
    Type: Application
    Filed: May 7, 2004
    Publication date: March 3, 2005
    Inventors: David Mann, Wilson Burgess, William Drohan, Yuri Griko, Martin MacPhee
  • Publication number: 20040013562
    Abstract: Methods are disclosed for sterilizing biological products to reduce the level of active biological contaminants such as viruses, bacteria, yeasts, molds, mycoplasmas and parasites.
    Type: Application
    Filed: July 18, 2002
    Publication date: January 22, 2004
    Inventors: Wilson Burgess, David Mann, Martin MacPhee, Randall Kent, William Drohan
  • Patent number: 6594002
    Abstract: A method to determine the systematic error of an instrument that measures features of a semiconductor wafer includes the following sequential steps. Collecting sensor data from measurement runs on front and back surfaces of a wafer while the wafer is oriented at different angles to the instrument for each run, yielding a front data set and a back data set for each angle. Then organizing the data in each set into a wafer-fixed coordinate frame. Reflecting all back surface data about a diameter of the wafer creates a reflected back data set. Subtracting the reflected back data from the front data for each wafer angle, and dividing the result by two, yields an averaged wafer shape for each load angle. Adding the reflected back data to the front data and dividing the result by two, yields an instrument signature for each load angle. The symmetric corrector is calculated by taking the average over all instrument signatures at each load angle.
    Type: Grant
    Filed: July 31, 2001
    Date of Patent: July 15, 2003
    Assignee: Ade Corporation
    Inventors: William Drohan, William Goldfarb, Peter Harvey, Jaydeep Sinha
  • Publication number: 20020184655
    Abstract: A method of producing a protein that degrades or detoxifies organic material is described. This method involves producing a non-human transgenic animal that produces such protein in its urine, and has stably integrated into its genome an exogenous gene encoding a protein that is detectable in the urine. Thus, a non-human transgenic animal that produced such protein in its urine, and a method of degrading or detoxifying organic materials also is described. Also a facility comprising a non-human transgenic animal that produce in its urine a protein that degrades or detoxifies organic material and a structure containing such animal is described. A method of altering a substance naturally found in urine is described. A DNA construct used in producing the non-human transgenic animal also is described.
    Type: Application
    Filed: December 1, 1997
    Publication date: December 5, 2002
    Inventors: HENRYK LUBON, REKHA PALEYANDA, WILLIAM DROHAN, WILLIAM VELANDER
  • Publication number: 20020017911
    Abstract: A method to determine the systematic error of an instrument that measures features of a semiconductor wafer includes the following sequential steps. Collecting sensor data from measurement runs on front and back surfaces of a wafer while the wafer is oriented at different angles to the instrument for each run, yielding a front data set and a back data set for each angle. Then organizing the data in each set into a wafer-fixed coordinate frame. Reflecting all back surface data about a diameter of the wafer creates a reflected back data set. Subtracting the reflected back data from the front data for each wafer angle, and dividing the result by two, yields an averaged wafer shape for each load angle. Adding the reflected back data to the front data and dividing the result by two, yields an instrument signature for each load angle. The symmetric corrector is calculated by taking the average over all instrument signatures at each load angle.
    Type: Application
    Filed: July 31, 2001
    Publication date: February 14, 2002
    Inventors: William Drohan, William Goldfarb, Peter Harvey, Jaydeep Sinha
  • Patent number: 5750908
    Abstract: A testing system with real time/off line functionality allocation is disclosed. The real time functions are limited to movement of objects to be tested through the testing stations, operation of sensors to gather raw data, and storage of the raw data. The off line functions include removing artifacts in the raw data, analyzing the raw data according to various rules, and controlling the sorting of the tested objects according to the analysis. By limiting the real time processing to a few essential elements necessary for operation of the testing line, the testing line can proceed even if the off line functions are not operating.
    Type: Grant
    Filed: February 25, 1997
    Date of Patent: May 12, 1998
    Assignee: ADE Corproation
    Inventor: William Drohan
  • Patent number: 4868113
    Abstract: Endothelial cell growth factor is achieved through the application of recombinant DNA technology to prepare cloning vehicles encoding the ECGF protein and procedures are disclosed for recovering ECGF protein essentially free of other proteins of human origin. The product is useful for, among other purposes, diagnostic applications and as potential in the treatment of damaged blood vessels or other endothelial cell-line structures.
    Type: Grant
    Filed: March 3, 1986
    Date of Patent: September 19, 1989
    Assignee: Rorer Biotechnology, Inc.
    Inventors: Michael Jaye, Wilson Burgess, Thomas Maciag, William Drohan