Patents by Inventor William A. Yirava
William A. Yirava has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200063178Abstract: This invention relates to a biological indicator for use with a sterilization process, comprising a carrier on which an inoculum is placed and dehydrated, the inoculum comprising a test organism in the form of bacterial spores and an effective amount of an excipient to enhance recovery of the bacterial spores from the dehydrated inoculum. In an embodiment, the inoculum further comprises a carbohydrate.Type: ApplicationFiled: August 21, 2018Publication date: February 27, 2020Inventors: William A. Yirava, Jodi R. Alt, Stephen M. Norris, Nicholas M. White
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Publication number: 20190382820Abstract: A recovery medium that is particularly suited for following enzymatic reactions where the fluorescent product is excited at a wavelength of 320 to 370 nm and emits at a wavelength of 420 to 460 nm is provided. The recovery medium is clear in color and does not contain the combination of compounds that participate in the Maillard reaction. The recovery medium is suitable for self-contained biological indicator systems.Type: ApplicationFiled: August 23, 2019Publication date: December 19, 2019Inventors: Kelly SOTO, Nicholas WHITE, Jennifer AUFMUTH, Sarah SMITH, Kathleen A. FIX, William A. YIRAVA
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Patent number: 10301631Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: GrantFiled: June 6, 2018Date of Patent: May 28, 2019Assignee: American Sterilizer CompanyInventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Patent number: 10301632Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: GrantFiled: June 6, 2018Date of Patent: May 28, 2019Assignee: American Sterilizer CompanyInventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Publication number: 20190106726Abstract: This invention relates to a biological indicator, comprising: a carrier; and a spore deposit on the carrier, wherein at least about 95% of the area of the spore deposit comprises spores residing in a single spore layer.Type: ApplicationFiled: October 11, 2017Publication date: April 11, 2019Inventors: Tricia Cregger, William Yirava, Phillip Franciskovich, Kathleen A. Fix
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Publication number: 20190106725Abstract: This invention relates to a biological indicator, comprising: a carrier; and a spore deposit on the carrier, wherein from about 70% to about 95% of the area of the spore deposit comprises spores residing in a single spore layer, and the remainder of the area of the spore deposit comprises spores residing in a stacked configuration.Type: ApplicationFiled: October 11, 2017Publication date: April 11, 2019Inventors: Tricia Cregger, William Yirava, Phillip Franciskovich, Kathleen A. Fix
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Publication number: 20180273957Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: ApplicationFiled: June 6, 2018Publication date: September 27, 2018Inventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Publication number: 20180273958Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: ApplicationFiled: June 6, 2018Publication date: September 27, 2018Inventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Publication number: 20180245122Abstract: A recovery medium that is particularly suited for following enzymatic reactions where the fluorescent product is excited at a wavelength of 320 to 370 nm and emits at a wavelength of 420 to 460 nm is provided. The recovery medium is clear in color and does not contain the combination of compounds that participate in the Maillard reaction. The recovery medium is suitable for self-contained biological indicator systems.Type: ApplicationFiled: February 23, 2018Publication date: August 30, 2018Inventors: Kelly SOTO, Nicholas WHITE, Jennifer AUFMUTH, Sarah SMITH, Kathleen A. FIX, William A. YIRAVA
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Patent number: 10017772Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: GrantFiled: June 9, 2017Date of Patent: July 10, 2018Assignee: American Sterilizer CompanyInventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Publication number: 20180187143Abstract: This invention relates to a biological indicator, comprising: a carrier inoculated with a test organism and an effective amount of a carbohydrate to reduce the resistance of the biological indicator to steam sterilization. The invention relates to a steam sterilization process using the biological indicator.Type: ApplicationFiled: March 1, 2018Publication date: July 5, 2018Inventors: William A. Yirava, Nicole M. Beahn, Phillip Franciskovich, Tricia Cregger
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Patent number: 10011843Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: GrantFiled: June 9, 2017Date of Patent: July 3, 2018Assignee: American Sterilizer CompanyInventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Patent number: 10011844Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: GrantFiled: June 9, 2017Date of Patent: July 3, 2018Assignee: American Sterilizer CompanyInventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Publication number: 20170275631Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: ApplicationFiled: June 9, 2017Publication date: September 28, 2017Inventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Publication number: 20170275633Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: ApplicationFiled: June 9, 2017Publication date: September 28, 2017Inventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Publication number: 20170275632Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: ApplicationFiled: June 9, 2017Publication date: September 28, 2017Inventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Publication number: 20170211035Abstract: This invention relates to a biological indicator, comprising: a carrier inoculated with a test organism and an effective amount of a carbohydrate to reduce the resistance of the biological indicator to steam sterilization. The invention relates to a steam sterilization process using the biological indicator.Type: ApplicationFiled: January 25, 2016Publication date: July 27, 2017Inventors: William A. Yirava, Nicole M. Beahn, Phillip Franciskovich, Tricia Cregger
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Patent number: 9701968Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: GrantFiled: July 7, 2016Date of Patent: July 11, 2017Assignee: American Sterilizer CompanyInventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Patent number: 9695428Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: GrantFiled: July 7, 2016Date of Patent: July 4, 2017Assignee: American Sterilizer CompanyInventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke
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Publication number: 20160312232Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.Type: ApplicationFiled: July 7, 2016Publication date: October 27, 2016Inventors: Phillip P. Franciskovich, Tricia A. Cregger, William A. Yirava, Peter A. Burke