Abstract: A method of forming an imaging calibration device for a biological material imaging system is provided. A first set of one or more discrete regions is provided upon or within a retaining member. Each said region of the first set comprises a selected tissue stain material, wherein each of the selected tissue stain materials exhibits a predetermined optical response inside a colour-stable region of a chromaticity diagram defined by the ellipse x2/a2+y2/b2=1, where a=0.21, b=0.135, the rotation is ?0.07 rad and the translation is (0.38,0.333). A second set of one or more discrete regions is also provided upon or within the retaining member. The second set is formed of one or more coloured filters having a predetermined optical response outside the colour-stable region.

Abstract: A method is provided of forming an imaging reference device for a biological material imaging system. A first region is provided in a substrate formed from an optically transmissive material which is an analogue of biological tissue. This first region is stained using a first biological tissue stain. A second region is provided in second substrate which is optically transmissive and an analogue of biological tissue. The second region is stained with a second biological tissue stain. The first and second regions are overlapped as a pair, such that light incident upon the pair is modulated by the respective first and second biological tissue stains of the respective regions. Multiple regions of the first and second type may be provided, stained to different degrees.

Abstract: A method is provided of forming a stain assessment target for a biological material staining system. At least one region of a substrate is provided upon a support such as a microscope slide. The substrate is formed from an optically transmissive material which is an analogue of biological tissue. A biological tissue sample for inspection is also provided upon the support. The support, including the at least one region, together with the biological tissue sample and then stained by applying at least one biological tissue stain under similar staining conditions.

Abstract: A method is provided of forming an imaging reference device for a biological material imaging system. A first region is provided in a substrate formed from an optically transmissive material which is an analogue of biological tissue. This first region is stained using a first biological tissue stain. A second region is provided in second substrate which is optically transmissive and an analogue of biological tissue. The second region is stained with a second biological tissue stain. The first and second regions are overlapped as a pair, such that light incident upon the pair is modulated by the respective first and second biological tissue stains of the respective regions. Multiple regions of the first and second type may be provided, stained to different degrees.

Abstract: A system for processing images includes an image file subsystem providing a source signal representing an input image; a color transformation subsystem coupled to the image file subsystem and accepting as input the source signal and producing a target signal therefrom, and an image forming subsystem coupled to the color transformation subsystem and forming a physical manifestation of the input image in response to the target signal, the color transformation subsystem being configured to establish a memory “cube” area representative of possible source signals, to define a sub-cube portion of the memory area as representative of the source signal; to determine possible target signals corresponding to the sub-cube portion; and to determine the target signal responsive to the possible target signals. If interpolation is found to be accurate for a mini-cube portion of the sub-cube, truncation or interpolation are used to derive the target signal; otherwise, the target signal is determined directly.

Abstract: A system for processing images includes an image file subsystem providing a source signal representing an input image; a color transformation subsystem coupled to the image file subsystem and accepting as input the source signal and producing a target signal therefrom, and an image forming subsystem coupled to the color transformation subsystem and forming a physical manifestation of the input image in response to the target signal, the color transformation subsystem being configured to establish a memory "cube" area representative of possible source signals, to define a sub-cube portion of the memory area as representative of the source signal; to determine possible target signals corresponding to the sub-cube portion; and to determine the target signal responsive to the possible target signals. If interpolation is found to be accurate for a mini-cube portion of the sub-cube, truncation or interpolation are used to derive the target signal; otherwise, the target signal is determined directly.