Abstract: Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

Abstract: A control system for an agricultural tillage implement includes a controller configured to receive a first pressure sensor signal indicative of a first fluid pressure within a rolling basket cylinder and a second pressure sensor signal indicative of a second fluid pressure within a wheel frame actuator. The rolling basket cylinder is configured to couple to a main frame and to a rolling basket frame, and the wheel frame actuator is configured to control a position of a wheel frame relative to a hitch assembly. The controller is also configured to decrease a target fluid pressure in response to the second fluid pressure being below a minimum wheel frame actuator pressure threshold, and the controller is configured to control a tilt force applied to the main frame to maintain the first fluid pressure within a range of the target fluid pressure.

Abstract: A control system for an agricultural tillage implement includes a controller configured to receive a first signal indicative of a target depth of a rear row of disc blades and a second signal indicative of a redirection amount and a redirection direction. The controller is configured to determine a target pitch angle adjustment based on the amount and direction, and the controller is configured to control a tilt actuator to control a tilt force applied to a main frame, a wheel actuator to control a position of a wheel relative to a hitch frame, and a rolling basket cylinder to control a position of a rolling basket relative to the main frame based on the target pitch angle adjustment, the target depth of the rear row of disc blades and one or more inverse kinematic relationships to establish a target penetration depth of a front row of disc blades.

Abstract: Indexed collar clamps allow for fast and easy adjustment of an associated telescoping stand, while preventing various underlying components from becoming dislodged from their respective positions over time. A telescoping stand may include an inner tube and an outer tube partially overlapping the inner tube. An overlapping end of the outer tube includes a compliance cut. A bushing is positioned between the inner tube and the outer tube and adjacent the compliance cut. The bushing includes a protrusion that indexes the bushing to an aperture in the outer tube. An indexed collar clamp is positioned over the outer tube and also adjacent the compliance cut. The protrusion from the bushing further indexes the bushing to a slot in the indexed collar clamp.

Abstract: A finishing tool control system for an agricultural implement may include a controller communicatively coupled to a first and a second sensor. The controller is configured to control a height of a finishing tool above a soil surface. Further, the first sensor is configured to output a signal indicative of a height of a main frame of the agricultural implement above the soil surface. In response to determining that a height increase of the main frame exceeds a threshold height adjustment, the controller increases the height of the finishing tool. The second sensor is configured to output a signal indicative of the height of the finishing tool of the finishing assembly above the soil surface. Moreover, in response to determining that a height of the finishing assembly above the soil surface is less than a threshold value, the controller increases the height of the finishing tool.

Abstract: A finishing tool control system for an agricultural implement may include a controller communicatively coupled to a first and a second sensor. The controller is configured to control a height of a finishing tool above a soil surface. Further, the first sensor is configured to output a signal indicative of a height of a main frame of the agricultural implement above the soil surface. In response to determining that a height increase of the main frame exceeds a threshold height adjustment, the controller increases the height of the finishing tool. The second sensor is configured to output a signal indicative of the height of the finishing tool of the finishing assembly above the soil surface. Moreover, in response to determining that a height of the finishing assembly above the soil surface is less than a threshold value, the controller increases the height of the finishing tool.

Abstract: Indexed collar clamps allow for fast and easy adjustment of an associated telescoping stand, while preventing various underlying components from becoming dislodged from their respective positions over time. A telescoping stand may include an inner tube and an outer tube partially overlapping the inner tube. An overlapping end of the outer tube includes a compliance cut. A bushing is positioned between the inner tube and the outer tube and adjacent the compliance cut. The bushing includes a protrusion that indexes the bushing to an aperture in the outer tube. An indexed collar clamp is positioned over the outer tube and also adjacent the compliance cut. The protrusion from the bushing further indexes the bushing to a slot in the indexed collar clamp.

Abstract: The clamping support heads disclosed herein provide a clampable mouth having jaws that can securely hold and permit movement of a variety of different size and/or shape objects. The objects may be quickly attached and quickly removed from the clamping support heads. A repositioning clutch allows for rotational repositioning of a clamped object. The repositioning clutch may include a clutch body attached to a stem of a stand, a friction ring seated to a clutch body, a bearing plate sharing a bearing interface with the friction ring, a clutch shaft extending through the clutch body, the friction ring, and the bearing plate to a terminal end, and a clutch adjustment handle screwed onto the terminal end of the clutch shaft. Rotation of the clutch adjustment handle adjusts compression force on the bearing interface between the friction ring and the bearing plate to secure or release the object from rotation.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

Abstract: In order for hydraulic disk brakes to function properly over time, associated brake pads are changed periodically. In order to exchange old brake pads for replacement brake pads, a disk brake may require pushing back the brake pistons within a brake caliper body in order to make room for installation of replacement pads. Current tools and methods typically require removal of a wheel from a vehicle, removal of a brake rotor from the wheel, and/or removal of the brake caliper body from a rotor. The presently disclosed caliper piston tool allows brake pads to be replaced without dismantling various components of the vehicle, resulting in significant time and cost savings as well as convenience for do-it-yourself and professional mechanics alike.

Abstract: Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

Abstract: In order for hydraulic disk brakes to function properly over time, associated brake pads are changed periodically. In order to exchange old brake pads for replacement brake pads, a disk brake may require pushing back the brake pistons within a brake caliper body in order to make room for installation of replacement pads. Current tools and methods typically require removal of a wheel from a vehicle, removal of a brake rotor from the wheel, and/or removal of the brake caliper body from a rotor. The presently disclosed caliper piston tool allows brake pads to be replaced without dismantling various components of the vehicle, resulting in significant time and cost savings as well as convenience for do-it-yourself and professional mechanics alike.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: A method for measuring the activity of one or more excitable cells, such as neurons, in a target tissue is provided. The present method may include measuring the activity of individual, selected excitable cells by projecting one or more three dimensional (3D) multi-focal laser light patterns into a target tissue containing excitable cells adapted to emit cellular electrical activity—sensitive fluorescence, to generate a multiplexed 2D diffraction pattern of fluorescence emitted by the neurons, and resolving the multiplexed 2D diffraction pattern. Also provided herein is a system configured to perform the present method, the system including a microscope configured to project one or more 3D multi-focal laser light patterns into a target tissue using a spatial light modulator and a mirror galvanometer, and a microlens array and an image detector to record individual and multiplexed 2D diffraction patterns of light emitted from the target tissue.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: A method for measuring the activity of one or more excitable cells, such as neurons, in a target tissue is provided. The present method may include measuring the activity of individual, selected excitable cells by projecting one or more three dimensional (3D) multi-focal laser light patterns into a target tissue containing excitable cells adapted to emit cellular electrical activity—sensitive fluorescence, to generate a multiplexed 2D diffraction pattern of fluorescence emitted by the neurons, and resolving the multiplexed 2D diffraction pattern. Also provided herein is a system configured to perform the present method, the system including a microscope configured to project one or more 3D multi-focal laser light patterns into a target tissue using a spatial light modulator and a mirror galvanometer, and a microlens array and an image detector to record individual and multiplexed 2D diffraction patterns of light emitted from the target tissue.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: A system and method for enabling a machine to drive through its parking brake when in first gear include detecting that the machine transmission is in its lowest gear in a selected direction, that the machine parking brake is activated, such that the machine is stationary, and that the throttle setting of the machine exceeds a predetermined throttle threshold value. In this state, one or more auxiliary loads of the machine are dropped to provide additional torque to the ground engaging elements of the machine, such that there is sufficient torque to drive the machine through the parking brake.