Abstract: A thermal cutting element of an electrosurgical instrument includes a base substrate defining a first side and a second side, at least one insulating layer disposed on at least the first side of the base substrate, and a heater circuit trace disposed on the at least one insulating layer. The thermal cutting element has an operating temperature of at least about 350° C. Temperature Coefficient of Resistance (TCR) of at least about 50 ppm/° C. An electrosurgical instrument and electrosurgical system including the thermal cutting element are also provided.

Abstract: Methods for treating coronavirus disease 2019 (COVID-19), the disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, are described. The methods can be used to reduce the severity of outcomes related to COVID-19, such as hospitalization and ventilation. For example, the methods can involve treatment of a subject with a therapeutic agent that degrades hyaluronan and/or an agent that neutralizes a hyaluronan receptor, e.g., CD44, such as an anti-CD44 antibody.

Abstract: A finishing tool control system for an agricultural implement may include a controller communicatively coupled to a first and a second sensor. The controller is configured to control a height of a finishing tool above a soil surface. Further, the first sensor is configured to output a signal indicative of a height of a main frame of the agricultural implement above the soil surface. In response to determining that a height increase of the main frame exceeds a threshold height adjustment, the controller increases the height of the finishing tool. The second sensor is configured to output a signal indicative of the height of the finishing tool of the finishing assembly above the soil surface. Moreover, in response to determining that a height of the finishing assembly above the soil surface is less than a threshold value, the controller increases the height of the finishing tool.

Abstract: The clamping support heads disclosed herein provide a clampable mouth having jaws that can securely hold and permit movement of a variety of different size and/or shape objects. The objects may be quickly attached and quickly removed from the clamping support heads. A repositioning clutch allows for rotational repositioning of a clamped object. The repositioning clutch may include a clutch body attached to a stem of a stand, a friction ring seated to a clutch body, a bearing plate sharing a bearing interface with the friction ring, a clutch shaft extending through the clutch body, the friction ring, and the bearing plate to a terminal end, and a clutch adjustment handle screwed onto the terminal end of the clutch shaft. Rotation of the clutch adjustment handle adjusts compression force on the bearing interface between the friction ring and the bearing plate to secure or release the object from rotation.

Abstract: Indexed collar clamps allow for fast and easy adjustment of an associated telescoping stand, while preventing various underlying components from becoming dislodged from their respective positions over time. A telescoping stand may include an inner tube and an outer tube partially overlapping the inner tube. An overlapping end of the outer tube includes a compliance cut. A bushing is positioned between the inner tube and the outer tube and adjacent the compliance cut. The bushing includes a protrusion that indexes the bushing to an aperture in the outer tube. An indexed collar clamp is positioned over the outer tube and also adjacent the compliance cut. The protrusion from the bushing further indexes the bushing to a slot in the indexed collar clamp.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

Abstract: In order for hydraulic disk brakes to function properly over time, associated brake pads are changed periodically. In order to exchange old brake pads for replacement brake pads, a disk brake may require pushing back the brake pistons within a brake caliper body in order to make room for installation of replacement pads. Current tools and methods typically require removal of a wheel from a vehicle, removal of a brake rotor from the wheel, and/or removal of the brake caliper body from a rotor. The presently disclosed caliper piston tool allows brake pads to be replaced without dismantling various components of the vehicle, resulting in significant time and cost savings as well as convenience for do-it-yourself and professional mechanics alike.

Abstract: Provided herein are devices, methods, and systems for in situ gene sequencing of a target nucleic acid in a cell in an intact tissue. Methods of screening a candidate agent to determine whether the candidate agent modulates gene expression of a nucleic acid in a cell in an intact tissue are also provided herein.

Abstract: In order for hydraulic disk brakes to function properly over time, associated brake pads are changed periodically. In order to exchange old brake pads for replacement brake pads, a disk brake may require pushing back the brake pistons within a brake caliper body in order to make room for installation of replacement pads. Current tools and methods typically require removal of a wheel from a vehicle, removal of a brake rotor from the wheel, and/or removal of the brake caliper body from a rotor. The presently disclosed caliper piston tool allows brake pads to be replaced without dismantling various components of the vehicle, resulting in significant time and cost savings as well as convenience for do-it-yourself and professional mechanics alike.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: A method for measuring the activity of one or more excitable cells, such as neurons, in a target tissue is provided. The present method may include measuring the activity of individual, selected excitable cells by projecting one or more three dimensional (3D) multi-focal laser light patterns into a target tissue containing excitable cells adapted to emit cellular electrical activity—sensitive fluorescence, to generate a multiplexed 2D diffraction pattern of fluorescence emitted by the neurons, and resolving the multiplexed 2D diffraction pattern. Also provided herein is a system configured to perform the present method, the system including a microscope configured to project one or more 3D multi-focal laser light patterns into a target tissue using a spatial light modulator and a mirror galvanometer, and a microlens array and an image detector to record individual and multiplexed 2D diffraction patterns of light emitted from the target tissue.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: A method for measuring the activity of one or more excitable cells, such as neurons, in a target tissue is provided. The present method may include measuring the activity of individual, selected excitable cells by projecting one or more three dimensional (3D) multi-focal laser light patterns into a target tissue containing excitable cells adapted to emit cellular electrical activity—sensitive fluorescence, to generate a multiplexed 2D diffraction pattern of fluorescence emitted by the neurons, and resolving the multiplexed 2D diffraction pattern. Also provided herein is a system configured to perform the present method, the system including a microscope configured to project one or more 3D multi-focal laser light patterns into a target tissue using a spatial light modulator and a mirror galvanometer, and a microlens array and an image detector to record individual and multiplexed 2D diffraction patterns of light emitted from the target tissue.

Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.

Abstract: A system and method for enabling a machine to drive through its parking brake when in first gear include detecting that the machine transmission is in its lowest gear in a selected direction, that the machine parking brake is activated, such that the machine is stationary, and that the throttle setting of the machine exceeds a predetermined throttle threshold value. In this state, one or more auxiliary loads of the machine are dropped to provide additional torque to the ground engaging elements of the machine, such that there is sufficient torque to drive the machine through the parking brake.

Abstract: A hydraulic fluid sampler and a method for obtaining a hydraulic fluid sample includes a sample container holder configured to support a sample container mounted thereto in an inverted position and a non-inverted position. In the inverted position, the sample container holder is configured to flush the sample container with hydraulic fluid so as to flush away any contaminates that may be present. In the non-inverted position, the sample container holder is configured to fill the sample container with hydraulic fluid.

Abstract: An overhung external spur gear positive displacement pump for hot oil or deep fryer applications is disclosed. The external gear pump includes a casing that includes a through bore for accommodating a drive shaft that leads to a pump chamber that accommodates a drive gear and a driven gear. The casing further includes a recess connected to the pump chamber for accommodating a stationary pin. The casing also includes a proximal end coupled to a prime mover and a distal end coupled to a head. The gears are enclosed in the pump chamber by the head. The stationary pin is accommodated within the driven gear and the drive shaft is coupled to the drive gear. The head is provided in the form of a flat plate with a plurality of holes for accommodating fasteners that couple the head to the distal end of the casing.

Abstract: A pump for use in a filtration process for a deep fryer is disclosed. The pump includes a drive shaft linked to a motor or motor drive shaft. The drive shaft passes through a seal assembly before passing into the pump chamber. The seal includes an outer seal housing that accommodates a lip seal. The lip seal is fabricated from PTFE. A combination oil filtration and supply system for a deep fryer is also disclosed that includes a motor linked to the drive shaft. The drive shaft passes through a supply pump and a filter/return pump. The supply pump includes an inlet in communication with a fresh oil reservoir and an outlet in communication with the filter/return pump. The filter/return pump includes an inlet in each communication with an oil filtration system and an outlet in communication with the outlet of the supply pump and an inlet to an oil distribution network. The oil distribution network includes a plurality of outlets, each in communication with an inlet of a vat.