Patents by Inventor William E. Werner
William E. Werner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8030096Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.Type: GrantFiled: August 12, 2010Date of Patent: October 4, 2011Assignee: Applied Biosystems LLCInventors: Linda G. Lee, Ronald Graham, Lily Lu, Elana E. Swartzman, William E. Werner
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Publication number: 20110081688Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.Type: ApplicationFiled: August 12, 2010Publication date: April 7, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Linda G. Lee, Ronald J. Graham, William E. Werner, Elana Swartzman, Lily Lu
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Patent number: 7795042Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.Type: GrantFiled: March 12, 2009Date of Patent: September 14, 2010Assignee: Applied Biosystems, LLCInventors: Linda G. Lee, Ronald J. Graham, William E. Werner, Elana Swartzman, Lily Lu
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Publication number: 20090305433Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.Type: ApplicationFiled: March 12, 2009Publication date: December 10, 2009Applicant: APPLIED BIOSYSTEMS INC.Inventors: LINDA G. LEE, RONALD J. GRAHAM, WILLIAM E. WERNER, ELANA SWARTZMAN, LILY LU
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Patent number: 7504496Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.Type: GrantFiled: February 14, 2005Date of Patent: March 17, 2009Inventors: Linda G. Lee, Ronald J. Graham, William E. Werner, Elana Swartzman, Lily Lu
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Patent number: 6787030Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also-be monitored to detect the presence of separated components in the equate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: GrantFiled: June 23, 2003Date of Patent: September 7, 2004Assignee: Applera CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan
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Publication number: 20040060872Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also-be monitored to detect the presence of separated components in the equate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: ApplicationFiled: June 23, 2003Publication date: April 1, 2004Applicant: Applera CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan
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Patent number: 6582597Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also be monitored to detect the presence of separated components in the eluate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: GrantFiled: June 19, 2001Date of Patent: June 24, 2003Assignee: Applera CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan
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Publication number: 20030055257Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.Type: ApplicationFiled: October 24, 2001Publication date: March 20, 2003Applicant: Applera CorporationInventors: Linda G. Lee, Ronald J. Graham, William E. Werner, Elana Swartzman, Lily Lu
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Patent number: 6372907Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.Type: GrantFiled: September 14, 2000Date of Patent: April 16, 2002Assignee: Apptera CorporationInventors: Linda G. Lee, Ronald J. Graham, William E. Werner, Elana Swartzman, Lily Lu
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Publication number: 20020011444Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also be monitored to detect the presence of separated components in the equate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: ApplicationFiled: June 19, 2001Publication date: January 31, 2002Applicant: The Perkin-Elmer CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan
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Patent number: 6331618Abstract: The invention provides compositions and methods suitable for dissolving nucleic acid analogs with uncharged, neutral backbones at high concentrations at approximately neutral pH. By using the compositions of the invention, which include a polar, aprotic solvent, concentrations of nucleic acid analogs such as PNA can be achieved in the range of approximately 1 &mgr;M to 10 mM.Type: GrantFiled: May 13, 1999Date of Patent: December 18, 2001Assignee: PE Corporation (NY)Inventors: William Bloch, William E. Werner, Michael Egholm, Rene L. Myers
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Patent number: 6248239Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also be monitored to detect the presence of separated components in the eluate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: GrantFiled: July 2, 1997Date of Patent: June 19, 2001Assignee: The Perkin-Elmer CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan
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Patent number: 6191278Abstract: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.Type: GrantFiled: November 3, 1999Date of Patent: February 20, 2001Assignee: PE CorporationInventors: Linda G. Lee, Ronald J. Graham, William E. Werner, Elana Swartzman, Lily Lu
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Patent number: 5264101Abstract: A capillary tube with a charged inner surface for use in capillary electrophoresis and methods. The tube is filled with an electrolyte solution containing 0.05-30% weight/weight of a hydrophilic polymer which is characterized by (A) a molecular weight of 20-5,000 kilodaltons, and (B) a charge between 0.01 and 1.0% as measured by the molar percent of charged monomer subunits to the total polymer subunits, where said charged monomer subunits have the charge opposite to the wall charge at a selected electrophoresis pH.Type: GrantFiled: May 1, 1992Date of Patent: November 23, 1993Assignee: Applied Biosystems, Inc.Inventors: David M. Demorest, William E. Werner, John E. Wiktorowicz