Patents by Inventor William Grady

William Grady has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS AND COMPOSITIONS FOR DETECTING CANCERS ASSOCIATED WITH METHYLATION OF HMLH1 PROMOTER DNA
    Publication number: 20140206855
    Abstract: Methods are provided for detection of cancers associated with methylation of hMLH1 promoter DNA in a subject. The method comprise assaying for the presence of methylated hMLH1 promoter DNA in a bodily fluid from a subject. In one embodiment, the method comprises reacting DNA from the sample with a chemical compound that converts non-methylated cytosine bases but not methylated cytosine bases, to a different nucleotide base. The compound-converted DNA is then amplified using a methylation-sensitive polymerase chain reaction (MSP) employing primers that amplify the compound-converted DNA template. The present invention also provides nucleotide primer sequences for use in the methylation-sensitive PCR assay.
    Type: Application
    Filed: January 16, 2014
    Publication date: July 24, 2014
    Applicant: CASE WESTERN RESERVE UNIVERSITY
    Inventors: Sanford D. Markowitz, William Grady
  • Methods and compositions for detecting cancers associated with methylation of promoter DNA
    Patent number: 8669060
    Abstract: Methods are provided for detection of cancers associated with methylation of hMLH1 promoter DNA in a subject. The method comprise assaying for the presence of methylated hMLH1 promoter DNA in a bodily fluid from a subject. In one embodiment, the method comprises reacting DNA from the sample with a chemical compound that converts non-methylated cytosine bases but not methylated cytosine bases, to a different nucleotide base. The compound-converted DNA is then amplified using a methylation-sensitive polymerase chain reaction (MSP) employing primers that amplify the compound-converted DNA template. The present invention also provides nucleotide primer sequences for use in the methylation-sensitive PCR assay.
    Type: Grant
    Filed: January 5, 2005
    Date of Patent: March 11, 2014
    Assignee: Case Western Reserve University
    Inventors: Sanford Markowitz, William Grady
  • CONTROLLED RELEASE STRUCTURE FOR ATTACHING MEDICAL DEVICES
    Publication number: 20070185461
    Abstract: A medical device for use with a fluid transfer device includes a hub having an open proximal end with a frusto-conically-shaped cavity therein, a distal end and a passageway therethrough. The cavity is part of the passageway. A release element in the passageway of the hub is positioned to block fluid-tight engagement of the frusto-conically-shaped tip with the cavity of the hub. Structure is provided to release at least part of the release element, upon application of a proximally directed force on the hub, to allow the abrupt fluid-tight engagement of the tip and the cavity in the hub.
    Type: Application
    Filed: January 4, 2007
    Publication date: August 9, 2007
    Applicant: BECTON, DICKINSON AND COMPANY
    Inventors: Richard Caizza, William Grady
  • CONTROLLED RELEASE STRUCTURE FOR ATTACHING MEDICAL DEVICES
    Publication number: 20070173776
    Abstract: A medical device for use with a fluid transfer device includes a hub having an open proximal end with a frusto-conically-shaped cavity therein, a distal end and a passageway therethrough. The cavity is part of the passageway. A release element in the passageway of the hub is positioned to block fluid-tight engagement of the frusto-conically-shaped tip with the cavity of the hub. Structure is provided to release at least part of the release element, upon application of a proximally directed force on the hub, to allow the abrupt fluid-tight engagement of the tip and the cavity in the hub.
    Type: Application
    Filed: January 4, 2007
    Publication date: July 26, 2007
    Applicant: BECTON, DICKINSON AND COMPANY
    Inventors: RICHARD CAIZZA, William Grady
  • Methods and compositions for detecting cancers associated with methylation of hMLH1 promoter DNA
    Publication number: 20050153351
    Abstract: Methods are provided for detection of cancers associated with methylation of hMLH1 promoter DNA in a subject. The method comprise assaying for the presence of methylated hMLH1 promoter DNA in a bodily fluid from a subject. In one embodiment, the method comprises reacting DNA from the sample with a chemical compound that converts non-methylated cytosine bases but not methylated cytosine bases, to a different nucleotide base. The compound-converted DNA is then amplified using a methylation-sensitive polymerase chain reaction (MSP) employing primers that amplify the compound-converted DNA template. The present invention also provides nucleotide primer sequences for use in the methylation-sensitive PCR assay.
    Type: Application
    Filed: January 5, 2005
    Publication date: July 14, 2005
    Inventors: Sanford Markowitz, William Grady
  • Methods and compositions for detecting cancers associated with methylation of hMLH1 promoter DNA
    Patent number: 6858388
    Abstract: Methods are provided for detection of cancers associated with methylation of hMLH1 promoter DNA in a subject. The method comprise assaying for the presence of methylated hMLH1 promoter DNA in a bodily fluid from a subject. In one embodiment, the method comprises reacting DNA from the sample with a chemical compound that converts non-methylated cytosine bases but not methylated cytosine bases, to a different nucleotide base. The compound-converted DNA is then amplified using a methylation-sensitive polymerase chain reaction (MSP) employing primers that amplify the compound-converted DNA template. The present invention also provides nucleotide primer sequences for use in the methylation-sensitive PCR assay.
    Type: Grant
    Filed: September 20, 2001
    Date of Patent: February 22, 2005
    Assignee: Case Western Reserve University
    Inventors: Sanford Markowitz, William Grady
  • Methods and compositions for detecting cancers associated with methylation of hMLH1 promoter DNA
    Publication number: 20030068620
    Abstract: Methods are provided for detection of cancers associated with methylation of hMLH1 promoter DNA in a subject. The method comprise assaying for the presence of methylated hMLH1 promoter DNA in a bodily fluid from a subject. In one embodiment, the method comprises reacting DNA from the sample with a chemical compound that converts non-methylated cytosine bases but not methylated cytosine bases, to a different nucleotide base. The compound-converted DNA is then amplified using a methylation-sensitive polymerase chain reaction (MSP) employing primers that amplify the compound-converted DNA template. The present invention also provides nucleotide primer sequences for use in the methylation-sensitive PCR assay.
    Type: Application
    Filed: September 20, 2001
    Publication date: April 10, 2003
    Inventors: Sanford Markowitz, William Grady