Abstract: The present disclosure provides antibodies and antigen-binding fragments thereof that bind specifically to a coronavirus spike protein and methods of using such antibodies and fragments for treating or preventing viral infections (e.g., coronavirus infections).

Abstract: The present disclosure relates to use of an acid for developing or treating a radiation-sensitive film including two or more elements having a high patterning radiation-absorption cross-section. The acid can be employed to form a pattern by a negative tone development process or to treat a developed pattern by further removing residual resist components.

Abstract: The present disclosure relates to use of aqueous acid for developing or treating a radiation-sensitive film. The aqueous acid can be employed to form a pattern by a positive tone wet development process or to treat a developed pattern by further removing residual resist components.

Abstract: The present disclosure relates to a film formed with an organometallic precursor and an organic co-reactant, as well as methods for forming and employing such films. In particular embodiments, the films can be incubated after exposure to radiation, which can provide enhanced material differences between the exposed and unexposed regions. In non-limiting embodiments, the radiation can include extreme ultraviolet (EUV) or deep ultraviolet (DUV) radiation.

Abstract: Provided herein are compounds having aqueous solubility, stability, and fluorescence, as well as methods of detecting the presence or absence of an analyte in a test sample using said compounds.

Abstract: Standard surfactants are known to negatively impact the detection of nucleic acids in amplification and other detection techniques. Provided herein are novel surfactants that are useful in applications such as nucleic acid amplification, and that are stable under the conditions of such applications.

Abstract: A method of controlling an agricultural facility for housing a plurality of animals includes the step of regulating the environment within the agricultural facility with an automated building operational system. An automated thermal event controller is operationally coupled with the automated building operational system and generates a thermal event signal in response to a thermal event in progress, whereby the automated thermal event controller, upon receipt of the thermal event signal indicating that the thermal event is in progress, activates an alarm state. In the alarm state, the automated thermal event controller: sends an alarm signal to a system administrator; places the automated building operational system in a safety-mode condition for a first predetermined time interval; and places the automated building operational system in a normal-mode operational condition after the first predetermined time interval and if a confirmatory signal is not yet then received from the system administrator.

Abstract: The disclosure relates, in part, to a method for detecting an analyte in a sample, comprising: contacting a substrate construct with a sample that may comprise the analyte, and using evanescent wave imaging to detect the analyte.

Abstract: The disclosure is directed to a compound of formula III: wherein: X is a heteroatom; Base is a nucleobase; R1 and R2 are each independently selected from the group consisting of H, CF3, CN, a C1-C12 straight chain or branched alkyl, a C2-C12 straight chain or branched alkenyl or polyenyl, a C2-C12 straight chain or branched alkynyl or polyalkynyl, a C1-C12 ether, and an aromatic group (e.g., a phenyl, a naphthyl, a pyridine), with the proviso that at least one of R1 and R2 is CF3, CN, a C1-C12 straight chain or branched alkyl, a C2-C12 straight chain or branched alkenyl or polyenyl, a C2-C12 straight chain or branched alkynyl or polyalkynyl, a C1-C12 ether, or an aromatic group (e.g., a phenyl, a naphthyl, a pyridine); R3 is NO2; R4 is H; R5 comprises a C1-C12 alkyne, an amide, and/or an amine; R6 is OMe or S—C6H6; and R7 is H or NO2.

Abstract: The disclosure is directed to a method for nucleic acid sequencing, comprising: contacting a substrate polynucleotide immobilized to a substrate with a protected nucleotide and a sequencing primer in a presence of a polymerase such that the polymerase incorporates the protected nucleotide into the sequencing primer, wherein the protected nucleotide comprises a detectable moiety and a photocleavable terminating moiety; using evanescent wave imaging to identify the protected nucleotide incorporated into the sequencing primer; and using evanescent wave imaging to cleave the photocleavable terminating moiety of the protected nucleotide.

Abstract: The disclosure is directed to a method for preparing a sample for evanescent wave imaging, comprising: isothermally amplifying a target nucleic acid present in the sample in a reservoir to produce one or more amplicons, wherein the one or more amplicons are immobilized to a bottom surface of the reservoir; and contacting the one or more amplicons with an aqueous solution comprising sequencing reagents comprising a pool of 3?-unblocked protected nucleotides.

Abstract: The disclosure is directed to an imaging device comprising: a reservoir having an interior lower surface; a substrate arranged below the reservoir, wherein at least a portion of an upper surface of the substrate forms the interior lower surface of the reservoir; a first light source arranged proximate to the substrate and configured to direct light into the substrate; an image sensor arranged below the substrate and configured to receive emission light produced within the reservoir.

Abstract: The disclosure is directed to a method for nucleic acid sequencing, comprising: using evanescent wave imaging to identify a 3?-unblocked protected nucleotide incorporated into a sequencing primer.

Abstract: The disclosure is directed to (i) controlling a first light source to emit a first light into a substrate on which substrate polynucleotides are immobilized, wherein a plurality of substrate polynucleotides are each annealed to a sequencing primer and bound with a polymerase, wherein the substrate polynucleotides are in a presence of a pool of protected nucleotides, and wherein each protected nucleotide comprises a detectable moiety and a photocleavable terminating moiety; (ii) processing a fluorescence signal to identify protected nucleotides incorporated in the sequencing primers; (iii) controlling a second light source to emit a second light into the substrate to cleave the detectable moieties from the incorporated protected nucleotides; (iv) determining one of both of: a percentage of the sequencing primers that incorporated a protected nucleotide and a percentage of the detectable moieties cleaved from the incorporated protected nucleotides; and (v) modifying one or more parameters of a sequencing primer

Abstract: Aspects of the disclosure relate to compositions and methods for amplifying and/or detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens) in a biological sample obtained from a subject. In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. In some embodiments, the methods comprise isothermal amplification of a target nucleic acid and subsequent detection of the amplification products.

Abstract: Methods include capturing intravascular ultrasound images. A drive motor is used to actively drive an ultrasound transducer at a set rotation speed. A temporary sensing window is created in which the ultrasound transducer is driven with a fixed drive signal. A plurality of signals from are received the ultrasound transducer during the temporary sensing window.

Abstract: Aspects of the disclosure relate to compositions and methods for amplifying and/or detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens) in a biological sample obtained from a subject. In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. In some embodiments, the methods comprise isothermal amplification of a target nucleic acid and subsequent detection of the amplification products.

Abstract: A method of controlling an agricultural facility for housing a plurality of animals includes the step of regulating the environment within the agricultural facility with an automated building operational system. An automated thermal event controller is operationally coupled with the automated building operational system and generates a thermal event signal in response to a thermal event in progress, whereby the automated thermal event controller, upon receipt of the thermal event signal indicating that the thermal event is in progress, activates an alarm state. In the alarm state, the automated thermal event controller: sends an alarm signal to a system administrator; places the automated building operational system in a safety-mode condition for a first predetermined time interval; and places the automated building operational system in a normal-mode operational condition after the first predetermined time interval and if a confirmatory signal is not yet then received from the system administrator.

Abstract: Aspects of the disclosure relate to devices and methods for amplifying and/or detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens) in a biological sample obtained from a subject, wherein the biological sample is combined with a diluent and/or matrix resolving agent.

Abstract: A method for operating a combined-cycle electrical power grid by: accepting, from combined cycle power generation participants, combined cycle offers for valid combined cycle configurations, each including identification of power generator type(s) for the configuration and the offers including at least three levels of parameter information pertaining for the valid combined cycle configurations, the three levels including, (a) resource level parameters that describe transition information between the valid combined cycle configurations, (b) configuration level parameters that describe operating parameters for each valid configuration; and (c) component level parameters that describe operating parameters for specific power generator components included in a valid configuration; selecting commitments from the combined cycle offers based upon the resource level parameters, the configuration level parameters and the component level parameters; and controlling dispatch of electricity based upon the commitments.