Abstract: Provided herein are compositions and methods for the synthesis, amplification, and in vitro transcription of full-length cDNA, or cDNA fragments. Methods are provided for reverse transcription of RNA and amplification for in vitro transcription. Further provided are method for loading of dendritic cells with the RNA and homologous lysate for immune stimulation.

Abstract: Provided herein are compositions and methods for the synthesis, amplification, and in vitro transcription of full-length cDNA, or cDNA fragments. Methods are provided for reverse transcription of RNA and amplification for in vitro transcription. Further provided are method for loading of dendritic cells with the RNA and homologous lysate for immune stimulation.

Abstract: Provided herein are compositions and methods for the synthesis, amplification, and in vitro transcription of full-length cDNA, or cDNA fragments. Methods are provided for reverse transcription of RNA and amplification for in vitro transcription. Further provided are method for loading of dendritic cells with the RNA and homologous lysate for immune stimulation.

Abstract: Provided herein are methods of expanding CD161+ T cells. Also provided are methods and compositions for generating modified CD161+ T cells comprising a chimeric antigen receptor (CAR). In particular aspects, CAR-expressing T cells are produced, expanded, and/or used in disease (e.g, cancer) treatments.

Abstract: Methods of providing a targeted immune response in a subject comprising administration of a dendritic cell population are provided. In some aspects, dendritic cells are administered in conjunction with a Type I interferon (INF), a TLR-7 agonist, a TLR-9 agonist, AIMp1, a TLR-3 agonist, a retinoic acid inducible gene-1 (RIG-1)-like receptor ligand or a cytosolic DNA (CDS) receptor ligand and/or are administered to a tissue site proximal to diseased tissue. Therapeutic dendritic cell compositions are likewise provided.

Abstract: The present disclosure relates to immunogenic formulations and their use for the treatment and prevention of T. cruzi infection, Chagas Disease, and chronic Chagas cardiomyopathy. In certain aspects, methods of producing antigen presenting cells comprising T. cruzi antigens are provided.

Abstract: Provided herein are compositions and methods for the synthesis, amplification, and in vitro transcription of full-length cDNA, or cDNA fragments. Methods are provided for reverse transcription of RNA and amplification for in vitro transcription. Further provided are method for loading of dendritic cells with the RNA and homologous lysate for immune stimulation.

Abstract: Certain embodiments of the invention are directed to methods for inducing an immunologic response to a tumor in a patient using mature dendritic cells transfected with a nucleic acid composition encoding one or more tumor antigens and loaded with a corresponding tumor antigen composition.

Abstract: Certain embodiments of the invention are directed to methods for inducing an immunologic response to a tumor in a patient using mature dendritic cells transfected with a nucleic acid composition encoding one or more tumor antigens and loaded with a corresponding tumor antigen composition.

Abstract: Methods of providing a targeted immune response in a subject comprising administration of a dendritic cell population are provided. In some aspects, dendritic cells are administered in conjunction with a Type I interferon (INF), a TLR-7 agonist, a TLR-9 agonist, AIMp1, a TLR-3 agonist, a retinoic acid inducible gene-1 (RIG-1)-like receptor ligand or a cytosolic DNA (CDS) receptor ligand and/or are administered to a tissue site proximal to diseased tissue. Therapeutic dendritic cell compositions are likewise provided.

Abstract: Methods are provided for enhancing an immune response comprising providing an immunogenic composition in conjunction with a CTLA-4 antagonist. Dendritic cell populations having reduced CTLA-4 expression are likewise provided.

Abstract: Certain embodiments of the invention are directed to methods for inducing an immunologic response to a tumor in a patient using mature dendritic cells transfected with a nucleic acid composition encoding one or more tumor antigens and loaded with a corresponding tumor antigen composition.

Abstract: Certain embodiments of the invention are directed to methods for inducing an immunologic response to a tumor in a patient using mature dendritic cells transfected with a nucleic acid composition encoding one or more tumor antigens and loaded with a corresponding tumor antigen composition.

Abstract: Certain embodiments of the invention are directed to methods for inducing an immunologic response to a tumor in a patient using mature dendritic cells transfected with a nucleic acid composition encoding one or more tumor antigens and loaded with a corresponding tumor antigen composition.

Abstract: Certain embodiments of the invention are directed to methods for inducing an immunologic response to a tumor in a patient using mature dendritic cells transfected with a nucleic acid composition encoding one or more tumor antigens and loaded with a corresponding tumor antigen composition.

Abstract: Certain embodiments of the invention are directed to methods for inducing an immunologic response to a tumor in a patient using mature dendritic cells transfected with a nucleic acid composition encoding one or more tumor antigens and loaded with a corresponding tumor antigen composition.

Abstract: A device for separating and collecting particulates from an air stream is provided. The device comprises a separation chamber housing a plurality of particulate separators. Each particulate separator in the plurality of particulate separators includes an intake port, a mechanical separation unit, a discharge port, and an outlet port. An air stream enters the particulate separator through the intake port. The mechanical separation unit is capable of separating particulates from the air stream and discharging the separated particulates through the discharge port. Clean air is expelled through the outlet ports of the plurality of particulate separators. The device further comprises an air stream entrance port capable of receiving the air stream, an air stream exit port capable of receiving the clean air, and a particulate reservoir capable of collecting discharged particulates.

Abstract: An air precleaner for mechanically removing particulate contaminants from an enclosed environment includes a peripheral air intake manifold that is in communication with the inlet port of a mechanical separation unit, and a central exhaust port that is in communication with the air output port of the mechanical separation unit. The precleaner can be inserted into or onto a vacuum system from which airborne particulate matter is to be removed. The precleaner includes a container which may be a two part container formed of a fitting assembly and a canister for the collection of particulates. The canister itself may be divided into a top and a bottom, compartment by a flange extending outwardly around the outer edge of the mechanical separation unit. Particulate matter collects in the bottom compartment of the canister, which can be periodically removed from the rest of the precleaner to dispose of the accumulated particulates.

Abstract: Intake air cleaning apparatus includes a filter canister with a filter mounted therein to filter air passing from an inlet end of the canister to an outlet end. A hood is mounted to the filter canister at the inlet end and has a discharge port for discharging particulates. A series of vanes are mounted in a gap between the hood and the inlet end of the filter canister to define channels through which air is directed into the air space between the hood and canister inlet end. A rotor mounted within the air space flings particulates toward the hood for expulsion through the discharge port. The filter within the canister may be formed of an outer cylindrical filter and an inner cylindrical filter mounted to the outlet wall of the canister to filter the precleaned air that passes through an inlet opening of the canister into the air space between the interior of the canister and the outer filter.

Abstract: An air precleaner includes a base assembly, a hood mounted to the base assembly and spaced from the base assembly to define a circumferential gap, and a rotor assembly mounted for rotation within the air space between the hood and base assembly. The base assembly has an outlet port that is connectable to an engine intake. Inlet ports may be formed between web supports connecting an outer circumferential wall to an inner circumferential wall of the base assembly. A series of vanes are mounted in the gap between the hood and base assembly at an angle between radial and tangential to define channels between them through which air is directed into the air space between the hood and base assembly with both an inward and tangential component of velocity. The air drawn in through the channels between the vanes provides a strong tangential component of air velocity impacting upon the rotor to maintain high efficiency of particulate removal.