Abstract: An insert for securing a threaded object in a recipient material. The insert is formed from a cylinder-shaped body. The body has a threaded cavity at its first end for receiving a threaded object. A portion of the cylinder's exterior surface adjacent to the first end is threaded for placing the insert in a hole in the recipient material and then stabilizing the insert therein. Additional stability is provided by the interface of a flange on the cylinder's second end with an adhesive which is placed in the hole with the insert. The flange is formed by a circumferential recess near the cylinder's second end. The adhesive occupies the recess and, when it hardens and bonds to the recipient material adjacent to the recess, serves as an circular barrier abutting the inner boundary of the flange. The barrier prevents the insert from being pulled from the hole.

Abstract: A dye binding reagent for protein assay comprises Coomassie Brilliant Blue G-250 and an acid having a pKa of from 1 to 2. Upon addition of the reagent to a protein-containing solution, the attendant color change can be spectrophotometrically measured to quantitate even micrograms of protein. Furthermore, the reagent is applicable to a wide range of proteins and requires only about 2 minutes for measurement. Addition of an alcohol such as ethanol to the reagent further improves sensitivity.