Abstract: A video distribution method modifies video to incorporate a tag that associates the video with an advertisement and facilitates payment of a share of advertising revenue to an owner of the video when the video and/or its associated advertisement are displayed at a client device.

Abstract: Systems and methods of the present invention may relate to enabling content item generators to present content items for association of advertisements; enabling an advertiser to request association of an advertisement with at least one of the presented content items; and tagging the content items so that upon a subsequent user interaction with a content item that has an associated advertisement, an action may be automatically taken to facilitate payment of a share of revenue for the associated advertisement to an account of the content item generator. Systems and methods of the present invention may relate to providing an advertisement revenue sharing tag for a content item that is generated by a content item generator and that has an associated advertisement, wherein upon a user interaction with a content item, an action may be automatically taken to facility payment of a share of revenue for the associated advertisement to an account of the content item generator.

Abstract: The present invention provides recombinant nucleic acid molecules, expression cassettes, and vectors useful for expression of polypeptides, including heterologous polypeptides, such as antigens, in bacteria. Some of the recombinant nucleic acid molecules, expression cassettes and vectors comprise codon-optimized sequences encoding the polypeptides and/or signal peptides. Some of the recombinant nucleic acid molecules, expression cassettes, and expression vectors comprise sequences encoding non-Listerial and/or non-secA1 signal peptides for secretion of the polypeptides. The invention also provides bacteria comprising the nucleic acid molecules, expression cassettes, and expression vectors, as well as compositions such as vaccines comprising the bacteria. Methods of making and using the bacteria, recombinant nucleic acid molecules, and expression cassettes are also provided.