Abstract: The present invention provides nucleotide and amino sequences of the lysosomal targeting pathway enzyme GlcNAc-phosphotransferase, methods of producing and methods of purifying this enzyme.

Abstract: The present invention provides nucleotide and amino sequences of the lysosomal targeting pathway enzyme GlcNAc-phosphotransferase, methods of producing and methods of purifying this enzyme.

Abstract: The present invention provides nucleotide and amino sequences of the lysosomal targeting pathway enzyme GlcNAc-phosphotransferase, methods of producing and methods of purifying this enzyme.

Abstract: The present invention provides highly phosphorylated lysosomal hydrolases, methods of modifying lysosomal hydrolases with the lysosomal targeting pathway enzymes GlcNAc-phosphotransferase and/or phosphodiester ?-GlcNAcase.

Abstract: The present invention provides nucleotide and amino sequences of the lysosomal targeting pathway enzyme phosphodiester &agr;-GlcNAcase, methods of producing and methods of purifying this enzyme.

Abstract: The present invention provides nucleotide and amino sequences of the lysosomal targeting pathway enzyme phosphodiester &agr;-GlcNAcase, methods of producing and methods of purifying this enzyme.

Abstract: The present invention provides highly phosphorylated lysosomal hydrolases, methods of modifying lysosomal hydrolases with the lysosomal targeting pathway enzymes GlcNAc-phosphotransferase and/or phosphodiester &agr;-GlcNAcase.

Abstract: Core 1 &bgr;3-galactosyl transferases and nucleic acids encoding the core 1 &bgr;3-galactosyl transferases or proteins having core 1 &bgr;3-galactosyl transferase activity or proteins having core 1 &bgr;3 galactosyl transferase activity are described. The enzymes and the nucleic acids encoding said enzymes have been identified in human, rat, mouse D. melanogaster and C. elegans. The polypeptides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational core 1 glycosylation of proteins or peptides produced within the expression system. The enzymes can be used to galactosylate, via a &bgr;3-linkage, an N-acetylgalactosamine linked to a serine, threonine or other O-linking amino acid on peptides or proteins requiring O-, N-, or S-linked glycosylation.

Abstract: The present invention provides nucleotide and amino sequences of the lysosomal targeting pathway enzyme GlcNAc-phosphotransferase, methods of producing and methods of purifying this enzyme.

Abstract: The present invention provides nucleotide and amino sequences of the lysosomal targeting pathway enzyme phosphodiester &agr;-GlcNAcase, methods of producing and methods of purifying this enzyme.

Abstract: The present invention provides a method of producing glycoproteins having reduced complex carbohydrates in a mammalian cell, glycoproteins produced by the method and cells that produce the glycoproteins.

Abstract: The present invention provides a method for producing high mannose glycoproteins in complex carbohydrate deficient cells and the glycoproteins obtained therein.

Abstract: Core 1 &bgr;3-galactosyl transferases and nucleic acids encoding the core 1 &bgr;3-galactosyl transferases are described. The enzymes and the nucleic acids encoding said enzymes have been identified in human, rat, mouse D. melanogaster and C. elegans. The polypeptides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational core 1 O-linked glycosylation of proteins or peptides produced within the expression system. The enzymes can be used to galactosylate, via a &bgr;3-linkage, an N-acetylgalactosamine linked to a serine, threonine or other 0-linking amino acid on peptides or proteins requiring O-linked glycosylation.

Abstract: The present invention provides methods of treating lysosomal storage disease with highly phosphorylated lysosomal hyrdrolases.

Abstract: The present invention provides highly phosphorylated lysosomal hydrolases, methods of modifying lysosomal hydrolases with the lysosomal targeting pathway enzymes GlcNAc-phosphotransferase and/or phosphodiester &agr;-GlcNAcase.

Abstract: Core 1 &bgr;3-galactosyl transferases and nucleic acids encoding the core 1 &bgr;3-galactosyl transferases are described. The enzymes and the nucleic acids encoding said enzymes have been identified in human, rat, mouse D. melanogaster and C. elegans. The polypeptides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational core 1 O-linked glycosylation of proteins or peptides produced within the expression system. The enzymes can be used to galactosylate, via a &bgr;3-linkage, an N-acetylgalactosamine linked to a serine, threonine or other O-linking amino acid on peptides or proteins requiring O-linked glycosylation.

Abstract: The present invention provides highly phosphorylated lysosomal hydrolases, methods of modifying lysosomal hydrolases with the lysosomal targeting pathway enzymes GlcNAc-phosphotransferase and/or phosphodiester &agr;-GlcNAcase.

Abstract: The present invention provides highly phosphorylated lysosomal hydrolases, methods of modifying lysosomal hydrolases with the lysosomal targeting pathway enzymes GlcNAc-phosphotransferase and/or phosphodiester 60 -GlcNAcase.

Abstract: The present invention provides a system and method for verifying an integrated circuit model. The model includes a plurality of net variables. The system and method comprises generating a plurality of tests for simulating the integrated circuit, precalculating a reduced model based upon the generated tests, and evaluating the reduced model. In a preferred embodiment, the present invention includes restricting the test that are generated. Then net invariants for the integrated circuit are generated by translating the restricted plurality of tests to a smaller set of possible values for the net variables. Thereafter, a minimization algorithm or procedure is utilized to minimize the logic used in the particular system based upon the latch constraints. This system produces a reduced model which reduces the amount of the integrated circuit that must be simulated thereby increasing the simulation speed thereof.