Abstract: A method of restoring an image of a computer, including identifying, by a backup application executing on the computer, artifacts of the computer to back up, creating a mirror image of a hard drive of the computer, wherein the mirror image comprises the artifacts, establishing a local environment within the computer, communicating with a backup server, by the backup application, using the local environment in the computer, backing up the mirror image, by the backup application, to the backup server using the local environment, updating the mirror image, by the backup application, in the backup server in response to a change in the computer, constructing the local environment on the computer in response to an event, loading a program from the local environment, and restoring the computer, by the backup application, from the mirror image in the backup server based on the artifacts, the program, and the restore process.

Abstract: A collection device is disclosed having a tubular housing detachably receiving a valved bulb reservoir at a first end thereof, the housing having a second end for dispensing fluids. The valved bulb reservoir has a sample capillary tube attached at one end thereof. The bore of the capillary is fluidically coupled to the valved bulb reservoir. The capillary is removed from the tubular housing to collect a sample in its bore, and is then replaced into the tubular housing. The contents of the valved tubular reservoir, which can be a buffer solution, a reagent, or other analytic fluid, or a gas, are then passed through the capillary bore to expel the sample and mix therewith in the housing. The sample product can then be dispensed from the tubular housing.

Abstract: A collection device is disclosed having a tubular housing detachably receiving a valved bulb reservoir at a first end thereof, the housing having a second end for dispensing fluids. The valved bulb reservoir has a sample capillary tube attached at one end thereof. The bore of the capillary is fluidically coupled to the valved bulb reservoir. The capillary is removed from the tubular housing to collect a sample in its bore, and is then replaced into the tubular housing. The contents of the valved tubular reservoir, which can be a buffer solution, a reagent, or other analytic fluid, or a gas, are then passed through the capillary bore to expel the sample and mix therewith in the housing. The sample product can then be dispensed from the tubular housing.

Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles.

Abstract: A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body.

Abstract: Method of repairing a seat cushion; and, in particular, method of repairing a flattened seat cushion having a fill which has been compressed over time, for example. Method of repairing a seat cushion include providing a cushion having a flattened fill, opening the cushion, removing the flattened fill, providing an inflatable insert, and inflating the inflatable insert to fill the cushion.

Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles.

Abstract: A magnetic mixing device designed to mix fluid in a reaction chamber and remove air bubbles if present. The device comprises a holder with embedded magnets, which causes movement of a stir bar within the reaction chamber. The holder may be moved by an electric linear actuator configured to generate linear motion or an electric motor configured to generate a circular motion. When orientated so the stir bar moves vertically within the reaction chamber, the stir bar disrupts any air bubbles trapped within or below the fluid.

Abstract: A magnetic mixing device designed to mix fluid in a reaction chamber and remove air bubbles if present. The device comprises a holder with embedded magnets, which causes movement of a stir bar within the reaction chamber. The holder may be moved by an electric linear actuator configured to generate linear motion or an electric motor configured to generate a circular motion. When orientated so the stir bar moves vertically within the reaction chamber, the stir bar disrupts any air bubbles trapped within or below the fluid.

Abstract: A magnetic mixing device designed to mix fluid in a reaction chamber and remove air bubbles if present. The device comprises a holder with embedded magnets, which causes movement of a stir bar within the reaction chamber. The holder may be moved by an electric linear actuator configured to generate linear motion or an electric motor configured to generate a circular motion. When orientated so the stir bar moves vertically within the reaction chamber, the stir bar disrupts any air bubbles trapped within or below the fluid.

Abstract: A magnetic mixing device designed to mix fluid in a reaction chamber and remove air bubbles if present. The device comprises a holder with embedded magnets, which causes movement of a stir bar within the reaction chamber. The holder may be moved by an electric linear actuator configured to generate linear motion or an electric motor configured to generate a circular motion. When orientated so the stir bar moves vertically within the reaction chamber, the stir bar disrupts any air bubbles trapped within or below the fluid.

Abstract: A self-metering reaction device has a sample reservoir, configured to accept a varying amount of fluid; a metering reservoir, configured to be a subportion of the sample reservoir and to hold a reaction amount of the fluid; a reaction chamber fluidly connected to the metering reservoir; and a plunger comprising a tip configured to make a seal with the metering reservoir so that the reaction amount of the fluid is sealed within the metering reservoir when the plunger is in contact with the metering reservoir. The plunger can be configured to plunge the sealed reaction amount of the fluid from the metering reservoir into the reaction chamber.

Abstract: Embodiments of present disclosure are directed to methods for amplifying nucleic acid, comprising two steps: a first step of preparing a reaction mixture comprising the target nucleic acid and a second step of processing the reaction mixture in a thermocycler. During a first phase of the processing step, the thermocycler may be configured to heat the reaction mixture to a first temperature and cool the reaction mixture to a second temperature repeatedly for a first plurality of cycles. During the first phase, fluorescence probes do not anneal to template strands and do not emit fluorescence signals. During a second phase of the processing step, the thermocycler may heat the reaction mixture to a third temperature and cool the reaction mixture to a fourth temperature repeatedly for a second plurality of cycles.

Abstract: A self-metering reaction device has a sample reservoir, configured to accept a varying amount of fluid; a metering reservoir, configured to be a subportion of the sample reservoir and to hold a reaction amount of the fluid; a reaction chamber fluidly connected to the metering reservoir; and a plunger comprising a tip configured to make a seal with the metering reservoir so that the reaction amount of the fluid is sealed within the metering reservoir when the plunger is in contact with the metering reservoir. The plunger can be configured to plunge the sealed reaction amount of the fluid from the metering reservoir into the reaction chamber.

Abstract: A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body.

Abstract: A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body.

Abstract: A collection device is disclosed having a tubular housing detachably receiving a valved bulb reservoir at a first end thereof, the housing having a second end for dispensing fluids. The valved bulb reservoir has a sample capillary tube attached at one end thereof. The bore of the capillary is fluidically coupled to the valved bulb reservoir. The capillary is removed from the tubular housing to collect a sample in its bore, and is then replaced into the tubular housing. The contents of the valved tubular reservoir, which can be a buffer solution, a reagent, or other analytic fluid, or a gas, are then passed through the capillary bore to expel the sample and mix therewith in the housing. The sample product can then be dispensed from the tubular housing.

Abstract: A magnetic mixing device designed to mix fluid in a reaction chamber and remove air bubbles if present. The device comprises a holder with embedded magnets, which causes movement of a stir bar within the reaction chamber. The holder may be moved by an electric linear actuator configured to generate linear motion or an electric motor configured to generate a circular motion. When orientated so the stir bar moves vertically within the reaction chamber, the stir bar disrupts any air bubbles trapped within or below the fluid.

Abstract: A self-metering reaction device has a sample reservoir, configured to accept a varying amount of fluid; a metering reservoir, configured to be a subportion of the sample reservoir and to hold a reaction amount of the fluid; a reaction chamber fluidly connected to the metering reservoir; and a plunger comprising a tip configured to make a seal with the metering reservoir so that the reaction amount of the fluid is sealed within the metering reservoir when the plunger is in contact with the metering reservoir. The plunger can be configured to plunge the sealed reaction amount of the fluid from the metering reservoir into the reaction chamber.

Abstract: A method and system for solid phase extraction of a compound of interest from a sample matrix using a syringe having a barrel and a plunger, a sorbent for use with the syringe, and a desalting purification column having an end configured to receive liquid from the syringe body.