Patents by Inventor William Nielsen
William Nielsen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11891661Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: GrantFiled: December 20, 2022Date of Patent: February 6, 2024Assignee: Roche Sequencing Solutions, Inc.Inventors: Kevin Aliado, Roger J. A. Chen, Jing Luo, William Nielsen, Kyle Umeda, Ashraf Wahba
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Publication number: 20240035083Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: ApplicationFiled: October 6, 2023Publication date: February 1, 2024Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, J. William Maney, JR., William Nielsen, Kyle Umeda, Ashraf Wahba
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Patent number: 11814676Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: GrantFiled: June 21, 2021Date of Patent: November 14, 2023Assignee: Roche Sequencing Solutions, Inc.Inventors: Kevin Aliado, Roger J. A. Chen, Jing Luo, J. William Maney, Jr., William Nielsen, Kyle Umeda, Ashraf Wahba
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Publication number: 20230185954Abstract: A system for generation and distribution of data handling requirements associated with sensitive data among a plurality of computing networks is described. Specifically, data generated at a first computing network may be tagged with associated data handling requirements. A reference library entry may be generated to include an indicator of the data and the associated data handling requirements. The reference library entry may be distributed to the plurality of computing networks, wherein each of the plurality of computing networks may add the reference library entry to corresponding reference libraries. Updates to the reference library entry, made at any one of the plurality of computing networks, may be propagated to other computing networks such that an updated listing of data handling requirements is accessible to users associated with the plurality of computing networks.Type: ApplicationFiled: December 15, 2021Publication date: June 15, 2023Inventors: Min Cao, Ryan William Nielsen, Eric Ryan Camarata
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Publication number: 20230120047Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: ApplicationFiled: December 20, 2022Publication date: April 20, 2023Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, William Nielsen, Kyle Umeda, Ashraf Wahba
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Patent number: 11530443Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: GrantFiled: June 3, 2020Date of Patent: December 20, 2022Assignee: Roche Sequencing Solutions, Inc.Inventors: Kevin Aliado, Roger J. A. Chen, Jing Luo, William Nielsen, Kyle Umeda, Ashraf Wahba
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Publication number: 20220291192Abstract: A consumable device used in a nanopore based sequencing system can include a nanopore chip, a flow cell with one or more flow channels, and a flow cell cover. A fluidic interface can be used to deliver fluid to the flow cell. The fluid interface can include a flow cell boss and the flow cell cover can include a receptacle for receiving the flow cell boss. A dispense tip can be used to introduce fluid into the flow cell through the flow cell boss.Type: ApplicationFiled: September 22, 2020Publication date: September 15, 2022Inventors: Matthew DiPietro, Damion Engelbart, Andrew Liu, William Nielsen, Janusz B. Wojtowicz, Robert A. Yuan
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Publication number: 20210310064Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: ApplicationFiled: June 21, 2021Publication date: October 7, 2021Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, J. William Maney, JR., William Nielsen, Kyle Umeda, Ashraf Wahba
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Patent number: 11041198Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: GrantFiled: September 20, 2019Date of Patent: June 22, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Kevin Aliado, Roger J. A. Chen, Jing Luo, J. William Maney, Jr., William Nielsen, Kyle Umeda, Ashraf Wahba
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Publication number: 20200291468Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: ApplicationFiled: June 3, 2020Publication date: September 17, 2020Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, William Nielsen, Kyle Umeda, Ashraf Wahba
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Patent number: 10683543Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: GrantFiled: November 12, 2018Date of Patent: June 16, 2020Assignee: Roche Molecular Systems, Inc.Inventors: Ashraf Wahba, William Nielsen, Jing Luo, Kevin Aliado, Kyle Umeda, Roger J.A. Chen
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Publication number: 20200032333Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: ApplicationFiled: September 20, 2019Publication date: January 30, 2020Inventors: Kevin Aliado, Roger J.A. Chen, Jing Luo, J. William Maney, JR., William Nielsen, Kyle Umeda, Ashraf Wahba
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Patent number: 10465240Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: GrantFiled: March 30, 2016Date of Patent: November 5, 2019Assignee: Roche Sequencing Solutions, Inc.Inventors: Ashraf Wahba, William Nielsen, J. William Maney, Jr., Kevin Aliado, Kyle Umeda, Roger J. A. Chen, Jing Luo
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Publication number: 20190144934Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: ApplicationFiled: November 12, 2018Publication date: May 16, 2019Inventors: Ashraf Wahba, William Nielsen, Jing Luo, Kevin Aliado, Kyle Umeda, Roger J. A. Chen
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Patent number: 10155979Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: GrantFiled: March 30, 2016Date of Patent: December 18, 2018Assignee: Roche Molecular Systems, Inc.Inventors: Ashraf Wahba, William Nielsen, Jing Luo, Kevin Aliado, Kyle Umeda, Roger J. A. Chen
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Publication number: 20170283866Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.Type: ApplicationFiled: March 30, 2016Publication date: October 5, 2017Inventors: Ashraf Wahba, William Nielsen, Jing Luo, Kevin Aliado, Kyle Umeda, Roger J.A. Chen
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Publication number: 20170283867Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.Type: ApplicationFiled: March 30, 2016Publication date: October 5, 2017Inventors: Ashraf Wahba, William Nielsen, J. William Maney, JR., Kevin Aliado, Kyle Umeda, Roger J.A. Chen, Jing Luo
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Publication number: 20140064946Abstract: A gas turbine engine compressor spacer (230) includes a body (229) with a hollow cylinder shape. The body (229) includes an outer surface (232) with an axially forward end. The body (229) also includes a forward face (233) extending radially inward from the axially forward end. A forward lip (237) extends axially from the body (229). The forward lip (237) includes a forward lip surface (239) located radially inward from the forward face (233). The forward lip surface (239) is the radially outer circumferential surface of the forward lip (237). The spacer (230) also includes a forward undercut (235). The forward undercut (235) is located between the forward face (233) and the forward lip surface (239).Type: ApplicationFiled: September 6, 2012Publication date: March 6, 2014Applicant: SOLAR TURBINES INCORPORATEDInventors: Engward William Nielsen, Kenneth Blair Hurst, Victor Manuel Siordia, II
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Patent number: 8584703Abstract: This invention provides composite plastic articles and methods of making them. The articles can be fluidic or microfluidic devices having fluidic conduits and, optionally, pneumatic conduits that regulate flow in the fluidic conduits. The articles comprise a first substrate coated with a layer of a material that comprises, or onto which have been introduced, reactive groups. For example, the substrate can be a plastic coated with an oxide or a siloxane onto which hydroxyl groups have been introduced. These articles are covalently bonded with other articles comprising reactive groups on their surfaces, for example, polysiloxanes treated to have silanol groups. Certain articles have specified locations on their surfaces that are not bonded to the other piece. For example, the coating can be removed from these locations before bonding. Such locations can be useful as functional elements of various devices, such as valve seats in valves of microfluidic devices.Type: GrantFiled: November 18, 2010Date of Patent: November 19, 2013Assignee: IntegenX Inc.Inventors: Boris Kobrin, Iuliu I. Blaga, William Nielsen, Shize D. Qi, Ezra Van Gelder
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Publication number: 20130214492Abstract: A system for forming a barrier between a lubrication oil region and an air region of a gas turbine engine comprising a lubrication oil return path, a centrifugal oil slinger on a rotating member fixed to a spool of the gas turbine engine, a spiral groove configured to directs lubrication oil toward an oil return path, a labyrinth seal, and a cavity configured to form a non-contacting seal with the spiral groove and the labyrinth seal.Type: ApplicationFiled: February 16, 2012Publication date: August 22, 2013Applicant: Solar Turbines IncorporatedInventors: Engward William Nielsen, Michael D. Fox