Abstract: A system and method for performing an assay for target analytes includes a sampling-device holder interface and a quantifier. The sampling device has a sampling portion and a reading portion containing a chemiluminescent reagent. The interface has a sampling-device holder and a light detector. The holder has a housing having an enclosure with a channel and a base plate with an opening. The base plate and the enclosure houses a slidable tray. The tray has a compartment adapted to seat the sampling device and an opening extending through the compartment. The reading portion is aligned with the tray opening. When the tray is closed, the tray opening is also aligned with the base plate opening, exposing the reading portion. Once the sampling portion is exposed to a sample suspected of containing the target analyte, at least one carrier liquid is introduced, if needed, to the sampling portion to transport any target analyte to the reading portion by dispersion.

Abstract: A method which utilize chemiluminescence for analyte detection and the detection of bacteria on surfaces. The method uses a device comprising a sampling portion made of a first adsorbent material, a container, and a second adsorbent material located within the container. The sampling portion collects analytes from a test sample which includes a surface or volume of a liquid. The second adsorbent material holds one or more chemiluminescent components including luciferase enzyme and cofactors in a dry state. In a preferred embodiment, the sampling portion is swabbed over a suspected contaminated surface. A bacteriolytic solution is then added to the adsorbent and releases ATP from any bacteria present. The ATP diffuses into the second adsorbent and mixes with the reagents therein to produce detectable light.