Abstract: Provided herein are nucleic acid molecules comprising artificial introns and modified replication coding sequences. Also provided are vectors and cells comprising the nucleic acid molecules comprising artificial introns and modified replication coding sequences.

Abstract: A reservoir assembly includes one or more pressure vessels each having a non-circular cross-sectional shape including a rounded rectangle having four generally flat sides with rounded corners. The pressure vessels may be formed of extruded metal, such as aluminum, and have a generally constant cross-section. The pressure vessels include stiffening ribs and varying wall thicknesses to improve strength and to minimize stresses when pressurized, such as during operation when filled with compressed gas. The stiffening ribs meet in the center of each of the pressure vessels and divide the interior volumes into four equal sections. A cap of stamped aluminum is fitted and fully welded to enclose each end of the pressure vessels. One or both of the caps on each of the pressure vessels has a pressure fitting. Two or more pressure vessels extend parallel to one another and are attached together to form the reservoir assembly.

Abstract: A reservoir assembly includes one or more pressure vessels each having a non-circular cross-sectional shape including a rounded rectangle having four generally flat sides with rounded corners. The pressure vessels may be formed of extruded metal, such as aluminum, and have a generally constant cross-section. The pressure vessels include stiffening ribs and varying wall thicknesses to improve strength and to minimize stresses when pressurized, such as during operation when filled with compressed gas. The stiffening ribs meet in the center of each of the pressure vessels and divide the interior volumes into four equal sections. A cap of stamped aluminum is fitted and fully welded to enclose each end of the pressure vessels. One or both of the caps on each of the pressure vessels has a pressure fitting. Two or more pressure vessels extend parallel to one another and are attached together to form the reservoir assembly.

Abstract: An embodiment is a sample rack system. The sample rack system comprises a rack having a base, a top opposite the base along a vertical direction, a receptacle that extends from the top toward the base along the vertical direction, and a receiver element proximate the top. The receptacle is sized to receive a sample collection unit. The system also includes a retention clip for holding the sample collection unit in the receptacle. The retention clip includes an engagement head for attachment to the receiver element of the rack. The engagement head defines an outer surface. The retention clip also includes a curved tail that extends from the engagement head and contacts the sample collection unit when the sample collection unit is placed in the receptacle. The outer surface of the retention clip does not project outwardly from the rack when the retention clip is attached to the rack.

Abstract: An embodiment is a sample analysis system for analyzing a sample that includes at least one test device for analyzing the sample. The system also includes a rack handler operable to move a sample from a first location to a second location along a travel path. The rack handler has a rotation assembly including a) an engagement element for engagement with the sample rack, and b) a motor coupled to the engagement element and is operable to cause rotation of the engagement element. The rotation assembly rotates the sample rack from a first orientation into a second orientation along the travel path when the engagement element engages the sample rack.

Abstract: Methods of purifying AAV particles using a chromatin-DNA nuclease (e.g., MNase), and compositions that include AAV particles and a chromatin-DNA nuclease (e.g., MNase) are described. Compositions and kits that include a chromatin-DNA nuclease (e.g., MNase) for the purification of AAV particles are also provided.

Abstract: An embodiment of the disclosure is a rack adapted to engage a rack handler. The rack includes a rack body. The rack body has a bottom, a top opposite the bottom, and a receptacle that extends from the top toward the bottom. The receptacle is sized to receive the sample collection unit. The rack body has a first interior surface that extends from the bottom toward the top, and a second interior surface that extends from the bottom toward the top. The second interior surface is opposite to the first interior surface so as to at least partially define a slot along the bottom. The rack body also includes an interference groove in the slot along at least one of the first interior surface and the second interior surface. The slot and the interference groove are sized to engage a portion of the rack handler.

Abstract: Absorbent laminated materials that include two spunbond nonwoven layers with a cellulose layer arranged in between the spunbond nonwoven layers are disclosed. The different layers of the absorbent laminated material are bonded through an ultrasonic treatment, and can further undergo an embossing step. In addition, the absorbent laminated materials may be used as disposable wiping products, among other applications.

Abstract: Absorbent laminated materials that include two spunbond nonwoven layers with a cellulose layer arranged in between the spunbond nonwoven layers are disclosed. The different layers of the absorbent laminated material are bonded through an ultrasonic treatment, and can further undergo an embossing step. In addition, the absorbent laminated materials may be used as disposable wiping products, among other applications.

Abstract: Provided herein are methods of using a nucleic acid construct as a selectable marker. The nucleic acid construct comprises an isolated ?-galactosidase expression cassette comprising a nucleic acid sequence encoding the amino-terminal fragment of ?-galactosidase operably linked to a promoter. Also provided are isolated vectors comprising the ?-galactosidase expression cassette, methods of generating the isolated vector, and kits comprising the isolated vector.

Abstract: A reservoir assembly includes one or more pressure vessels each having a non-circular cross-sectional shape including a rounded rectangle having four generally flat sides with rounded corners. The pressure vessels may be formed of extruded metal, such as aluminum, and have a generally constant cross-section. The pressure vessels include stiffening ribs and varying wall thicknesses to improve strength and to minimize stresses when pressurized, such as during operation when filled with compressed gas. The stiffening ribs meet in the center of each of the pressure vessels and divide the interior volumes into four equal sections. A cap of stamped aluminum is fitted and fully welded to enclose each end of the pressure vessels. One or both of the caps on each of the pressure vessels has a pressure fitting. Two or more pressure vessels extend parallel to one another and are attached together to form the reservoir assembly.

Abstract: Absorbent laminated materials that include two spunbond nonwoven layers with a cellulose layer arranged in between the spunbond nonwoven layers are disclosed. The different layers of the absorbent laminated material are bonded through an ultrasonic treatment, and can further undergo an embossing step. In addition, the absorbent laminated materials may be used as disposable wiping products, among other applications.

Abstract: Absorbent laminated materials that include two spunbond nonwoven layers with a cellulose layer arranged in between the spunbond nonwoven layers are disclosed. The different layers of the absorbent laminated material are bonded through an ultrasonic treatment, and can further undergo an embossing step. In addition, the absorbent laminated materials may be used as disposable wiping products, among other applications.

Abstract: An embodiment is a sample rack system. The sample rack system comprises a rack having a base, a top opposite the base along a vertical direction, a receptacle that extends from the top toward the base along the vertical direction, and a receiver element proximate the top. The receptacle is sized to receive a sample collection unit. The system also includes a retention clip for holding the sample collection unit in the receptacle. The retention clip includes an engagement head for attachment to the receiver element of the rack. The engagement head defines an outer surface. The retention clip also includes a curved tail that extends from the engagement head and contacts the sample collection unit when the sample collection unit is placed in the receptacle. The outer surface of the retention clip does not project outwardly from the rack when the retention clip is attached to the rack.

Abstract: An embodiment is a sample analysis system for analyzing a sample that includes at least one test device for analyzing the sample. The system also includes a rack handler operable to move a sample from a first location to a second location along a travel path. The rack handler has a rotation assembly including a) an engagement element for engagement with the sample rack, and b) a motor coupled to the engagement element and is operable to cause rotation of the engagement element. The rotation assembly rotates the sample rack from a first orientation into a second orientation along the travel path when the engagement element engages the sample rack.

Abstract: An embodiment of the disclosure is a rack adapted to engage a rack handler. The rack includes a rack body. The rack body has a bottom, a top opposite the bottom, and a receptacle that extends from the top toward the bottom. The receptacle is sized to receive the sample collection unit. The rack body has a first interior surface that extends from the bottom toward the top, and a second interior surface that extends from the bottom toward the top. The second interior surface is opposite to the first interior surface so as to at least partially define a slot along the bottom. The rack body also includes an interference groove in the slot along at least one of the first interior surface and the second interior surface. The slot and the interference groove are sized to engage a portion of the rack handler.

Abstract: Absorbent laminated materials that include two spunbond nonwoven layers with a cellulose layer arranged in between the spunbond nonwoven layers are disclosed. The different layers of the absorbent laminated material are bonded through an ultrasonic treatment, and can further undergo an embossing step. In addition, the absorbent laminated materials may be used as disposable wiping products, among other applications.

Abstract: A method for crystallizing insulin or insulin analogs under alkaline conditions and purifying the insulin or insulin analog crystals by filtering through a filter and drying the insulin or insulin analog crystals captured on the filter to produce crystalline insulin or insulin analog crystal compositions is described. In particular aspects, the method may be used to crystalize insulin glargine.

Abstract: A method for crystallizing insulin or insulin analogs under alkaline conditions and purifying the insulin or insulin analog crystals by filtering through a filter and drying the insulin or insulin analog crystals captured on the filter to produce crystalline insulin or insulin analog crystal compositions is described. In particular aspects, the method may be used to crystalize insulin glargine.

Abstract: A system is disclosed for recording continuous streaming data. The system includes a data collection unit, a wireless data transmission unit, a wireless data reception unit and a recorder unit. The data collection unit is for continuously collecting data at a data collection frequency, ƒC and providing collected data. The wireless data transmission unit is for continuously transmitting the collected data at a data transmission frequency, ƒT where ƒC is greater than ƒT. The wireless data reception unit is for continuously receiving collected data at a data reception frequency, ƒR where ƒR is equal to ƒT. The recorder unit is for providing a recorder output of the collected data at a frequency of ƒO where ƒO is equal to ƒT.