Abstract: An online liquid autosampler may include a pipette, a depyrogenation system, a sample source, and a movement system. The depyrogenation system may be configured to selectively depyrogenate the pipette. The sample source may be configured to provide a liquid to be sampled to the pipette. The movement system may be coupled to one or more of the pipette, the depyrogenation system, or the sample source. The movement system may be configured to: position the pipette within the depyrogenation system for depyrogenation; position a tip of the pipette within the sample source to aspirate a sample of the liquid from the sample source; and position the pipette to dispense an aliquot of the sample of the liquid into an aliquot sample target.

Abstract: A method to analyze a sample includes performing sample interrogation cycles on the sample to generate replicates. Each of the sample interrogation cycles is performed by: illuminating the sample with two or more fluorescence excitation signals at different wavelengths; and detecting both a fluorescence emission spectral profile and a fluorescence lifetime profile of the sample for each of the two or more fluorescence excitation signals to generate two or more fluorescence emission spectral profiles and two or more fluorescence lifetime profiles of the sample. Each replicate includes the two or more fluorescence emission spectral profiles and the two or more fluorescence lifetime profiles generated for a corresponding one of the sample interrogation cycles. The method includes performing a comparison of the replicates to predetermined spectroscopic relationships.