Abstract: Provided herein, in certain embodiments, are programmable nucleases, guide nucleic acids, and complexes thereof. Certain programmable nucleases provided herein comprise a RuvC domain. Also provided herein are nucleic acids encoding said programmable nucleases and guide nucleic acids. Also provided herein are methods of genome editing, methods of regulating gene expression, and methods of detecting nucleic acids with said programmable nucleases and guide nucleic acids.

Abstract: The preferred embodiment of present invention is directed to an improved method and system for buffering incoming/unsolicited data received by a host computer that is connected to a network such as a storage area network. Specifically, in a host computer system in which the main memory of the host server maintains a I/O control block command ring, and which a connective port (e.g., a host bus adaptor) is operatively coupled to the main memory for handling I/O commands received by and transmitted from the host server, a host buffer queue (HBQ) is maintained for storing a series of buffer descriptors retrievable by the port for writing incoming/unsolicited data to specific address locations within the main memory.