Abstract: The present disclosure provides a microbe engineering platform that permits optimization of microbe fitness levels to optimize a microbe's suitability for industrial fermentation. The disclosed platform identifies an association between microbe properties and microbe fitness levels. The association between microbe properties and microbe fitness levels may be used to identify candidate microbes with desired fitness levels. The identified candidate microbes may be used to further optimize the industrial fermentation process.

Abstract: The present disclosure provides a microbe engineering platform that permits optimization of microbe fitness levels to optimize a microbe's suitability for industrial fermentation. The disclosed platform identifies an association between microbe properties and microbe fitness levels. The association between microbe properties and microbe fitness levels may be used to identify candidate microbes with desired fitness levels. The identified candidate microbes may be used to further optimize the industrial fermentation process.

Abstract: Aspects of this disclosure relate to liquid-based workflows for strain engineering. In strain engineering, cells undergo a modification process to acquire a desired change or changes in the cells. Some of the cells accept the change or changes and some do not. Provided herein are liquid-based methods for selecting the population of cells that have accepted the change or changes. Also provided herein are liquid-based methods for removing markers from transformed cells and liquid-based methods for isolating clonally pure populations of cells.

Abstract: Disclosed are apparatuses, systems, and methods for processing materials, including biological or chemical materials. A manufacturing module may include a work station configured to perform a process involving equipment capable of use with biological or chemical material, a first transportation segment that is configured to connect and disconnect with a second transportation segment such that when connected, a carrier of the biological or chemical material can be transported from the first transportation segment to the second transportation segment, a pick and place robot configured to move an element between the first transportation segment and the work station; and a movement mechanism configured to allow the work station, the first transportation segment, and the pick and place robot to be moved as a single unit. A system may include a plurality of manufacturing modules that are reconfigurable to a plurality of configurations.

Abstract: The present disclosure provides a microbe engineering platform that permits optimization of microbe fitness levels to optimize a microbe's suitability for industrial fermentation. The disclosed platform identifies an association between microbe properties and microbe fitness levels. The association between microbe properties and microbe fitness levels may be used to identify candidate microbes with desired fitness levels. The identified candidate microbes may be used to further optimize the industrial fermentation process.

Abstract: Aspects of this disclosure relate to liquid-based workflows for strain engineering. In strain engineering, cells undergo a modification process to acquire a desired change or changes in the cells. Some of the cells accept the change or changes and some do not. Provided herein are liquid-based methods for selecting the population of cells that have accepted the change or changes. Also provided herein are liquid-based methods for removing markers from transformed cells and liquid-based methods for isolating clonally pure populations of cells.