Abstract: A method for catalytically converting a dihydrotetrazine 1 into a tetrazine 2, wherein the dihydrotetrazine 1 comprises a first R group and a second R group, wherein the first R group is a substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, or heteroatom-containing group, and the second R group is selected from the group consisting of H and substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, and heteroatom-containing groups; wherein the method comprises (a) providing the dihydrotetrazine 1 in a reaction medium, and (b) adding an enzyme as a catalyst and an oxidant to the reaction medium, whereby the dihydrotetrazine 1 is converted to the tetrazine 2.

Abstract: A method for catalytically converting a dihydrotetrazine 1 into a tetrazine 2, wherein the dihydrotetrazine 1 comprises a first R group and a second R group, wherein the first R group is a substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, or heteroatom-containing group, and the second R group is selected from the group consisting of H and substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, and heteroatom-containing groups; wherein the method comprises (a) providing the dihydrotetrazine 1 in a reaction medium, and (b) adding an enzyme as a catalyst and an oxidant to the reaction medium, whereby the dihydrotetrazine 1 is converted to the tetrazine 2.

Abstract: A method of managing a spreadtree hierarchy comprises storing at least one spreadsheet in a network environment accessible to a plurality of users, and storing at least one data object in the network responsive to an edit of the at least one spreadsheet, the at least one data object having a unique ID and data associated with the edit. A spreadtree system comprises an application server configured to operate within a network (Internet or Intranet), and further configured to communicate with a plurality of clients and manage a spreadtree hierarchy. The spreadtree hierarchy comprises a plurality of linked spreadsheets having reference files stored within the network, and a plurality of data objects associated with the plurality of linked spreadsheets. Each data object of the plurality includes a change to at least one cell in at least one linked spreadsheet of the plurality. A method for operating a spreadtree hierarchy system is also disclosed.