Abstract: After separation and during centrifuging a separated blood component is transferred from a source reservoir (2) into a tube (4) oriented against a centrifugal gradient. A cap (34) converts the separated layer from the reservoir into a columnar body and the centrifugal gradient prevents mixing of the columnar body in the tube (4) during further centrifuging. In one embodiment the tube has a volume sufficient to hold a desired fractional component from the source reservoir, and a diameter small enough to prevent mixing of the component when the gradient is removed. A support (69) holds the conduit wound about a spool transverse to the iso-g-lines of the centrifuge. The support (69) may be removably affixed to the cap (34). In a preferred embodiment the source reservoir (2) has a flexible wall, and pressure of the wall against an elevation (29) maintains pressure in the fluid system.

Abstract: Apparatus for the centrifugal fractionation and component-isolation of liquid material has a nonflexing radially-movable pressure panel disposed between a first liquid container stacked radially in front of a second liquid container. The two containers are at the same interval pressure during centrifugation, and the pressure panel avoids radial distortion of the back wall of the first container. The liquid container structure can incorporate the pressure panel. Alternatively, the panel can be part of the centrifugal separating instrument.